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Anti ar fl antibody c19

Manufactured by Santa Cruz Biotechnology
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The Anti-AR-FL antibody (C19) is a lab equipment product produced by Santa Cruz Biotechnology. It is a polyclonal antibody that recognizes the full-length androgen receptor (AR-FL) protein. This antibody can be used for the detection and analysis of the AR-FL protein in various experimental applications.

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Lab products found in correlation

Anti flag, by Merck Group (2 mentions) Duolink detection kit, by Merck Group (2 mentions)

Spelling variants of this product

Anti-AR (49 citations) Anti-AR antibody (21 citations) AR antibody (10 citations) Anti-AR (C19) (3 citations)

2 protocols using anti ar fl antibody c19

1

In Situ Proximity Ligation for AR

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Cells grown on coverslips were fixed with 10% formalin for 20 min and permeabilized with 0.5% Triton X-100 for 15 min at room temperature. AR-V7 and AR-FL were detected using an anti-FLAG (Sigma-Aldrich, F3165) and anti-AR-FL antibody (C19, Santa Cruz; against AR C-terminus), respectively. In situ proximity ligation was performed using a Duolink Detection Kit (Sigma-Aldrich). Nuclear foci were imaged using a Lionheart FX microscope (×40 magnification). The number of nuclear foci/cell was quantified using ImageJ with >50 cells analyzed per experiment per condition.
Roggero C.M., Jin L., Cao S., Sonavane R., Kopplin N.G., Ta H., Ekoue D.N., Witwer M., Ma S., Liu H., Ma T., Gioeli D., Raj G.V, & Dong Y. (2020). A detailed characterization of stepwise activation of the androgen receptor variant 7 in prostate cancer cells. Oncogene, 40(6), 1106-1117.
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2

In Situ Proximity Ligation for AR

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells grown on coverslips were fixed with 10% formalin for 20 min and permeabilized with 0.5% Triton X-100 for 15 min at room temperature. AR-V7 and AR-FL were detected using an anti-FLAG (Sigma-Aldrich, F3165) and anti-AR-FL antibody (C19, Santa Cruz; against AR C-terminus), respectively. In situ proximity ligation was performed using a Duolink Detection Kit (Sigma-Aldrich). Nuclear foci were imaged using a Lionheart FX microscope (×40 magnification). The number of nuclear foci/cell was quantified using ImageJ with >50 cells analyzed per experiment per condition.
Roggero C.M., Jin L., Cao S., Sonavane R., Kopplin N.G., Ta H., Ekoue D.N., Witwer M., Ma S., Liu H., Ma T., Gioeli D., Raj G.V, & Dong Y. (2020). A detailed characterization of stepwise activation of the androgen receptor variant 7 in prostate cancer cells. Oncogene, 40(6), 1106-1117.
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