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Ccr10 apc

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The CCR10-APC is a flow cytometry reagent that is used to detect and quantify the expression of the CCR10 receptor on the surface of cells. CCR10 is a chemokine receptor that is involved in the homing of T cells and B cells to the skin and other mucosal tissues. The CCR10-APC reagent consists of an anti-CCR10 antibody conjugated to the fluorescent dye APC (allophycocyanin), which allows for the detection and quantification of CCR10-positive cells by flow cytometry.

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Lab products found in correlation

Cd4 pe cy7, by BioLegend (4 mentions) Cd45ra pe, by BioLegend (4 mentions) Cd99 fitc, by BioLegend (4 mentions) Cd45ro apc, by BioLegend (2 mentions) Ccr10 apc, by R&D Systems (2 mentions) Hla dr apc, by BioLegend (2 mentions) Cd25 apc, by BioLegend (2 mentions) Fluorescein isothiocyanate (fitc), by BioLegend (2 mentions) Fixable viability dye efluor 780, by Thermo Fisher Scientific (2 mentions) Cd69 apc, by BioLegend (2 mentions) Cd69 bv421, by BioLegend (2 mentions) Cd3 af700, by BioLegend (2 mentions) Mouse fitc cd4 and efluor 605 cd44, by BD (2 mentions) Brilliant violet 570 cd4, by BioLegend (2 mentions) Alexa fluor 700 cd44, by BioLegend (2 mentions) Fitc klotho, by Bioss Antibodies (2 mentions) Pe cy7 cd99, by Bioss Antibodies (2 mentions) Pe cy5 vdr, by Bioss Antibodies (2 mentions) Pe cy7 cnr2, by Bioss Antibodies (2 mentions) Apc itga3, by R&D Systems (2 mentions)

5 protocols using ccr10 apc

1

Isolation and Sorting of Resting CD4 T Cells

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Peripheral blood mononuclear cells (PBMCs) from HIV- and HCV- healthy donors were isolated via Ficoll-paque density centrifugation. CD4 T cells were then isolated by negative selection (Stem Cell, Easy Sep) and resting CD4 T cells were obtained from this population by depleting cells expressing HLA-DR, CD25 and CD69 (Miltenyi). Resting CD4 T cells were stained in bulk for 1) CD99-FITC (Biolegend), CCR10-APC (BD Biosciences), and Propridium Iodide to distinguish viable cells; 2) CD4-PeCy7 only (Biolegend); or 3) CD45RA-PE (Biolegend) and CD45RO-APC (Biolegend). These three populations of cells were sorted independently on the MoFlo.
Song N., Sengupta S., Khoruzhenko S., Welsh R.A., Kim A., Kumar M.R., Sønder S.U., Sidhom J.W., Zhang H., Jie C., Siliciano R.F, & Sadegh-Nasseri S. (2020). Multiple genetic programs contribute to CD4 T cell memory differentiation and longevity by maintaining T cell quiescence. Cellular immunology, 357, 104210.
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2

Multiparametric Flow Cytometry Immunophenotyping

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Mouse FITC-CD4 and eFluor 605-CD44 [BD-Pharmingen]; KJ1.26 anti-TCR (Caltag Laboratories), Brilliant Violet 570-CD4 and Alexa Fluor 700-CD44 were from [Biolegend], FITC-Klotho,PE-Cy7-CD99, PE-Cy5-VDR, and PE-Cy7-CNR2 were from [Bioss]; APC-CCR10 and APC-ITGA3 were from [R&D Systems]; Fixable viability dye eFluor 780 was from [eBioscience]. Antibodies for human PBMC staining were as follows: from Biolegend, CD99-FITC,CD69-BV421 and CD69-APC , CD45R0-APC and CD45R0-BV421, CD3-AF700, CD4-Pe/Cy7, CD45RA-PE, Itga3 (CD49c)-PE, IL-7R-BV510, CD25-APC, HLA-DR-APC. From BD Biosciences, CCR10-APC and CCR10-PerCP Cy5.5. and viability dye eFluor 780 as above. Relevant mouse anti human isotype antibodies to IgG1k or IgG2ak were utilized for the following fluorophores: BV421, BV510, FITC, PE, and APC for both isotypes (Biolegend).
Song N., Sengupta S., Khoruzhenko S., Welsh R.A., Kim A., Kumar M.R., Sønder S.U., Sidhom J.W., Zhang H., Jie C., Siliciano R.F, & Sadegh-Nasseri S. (2020). Multiple genetic programs contribute to CD4 T cell memory differentiation and longevity by maintaining T cell quiescence. Cellular immunology, 357, 104210.
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3

Isolation and Sorting of Resting CD4 T Cells

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Peripheral blood mononuclear cells (PBMCs) from HIV- and HCV- healthy donors were isolated via Ficoll-paque density centrifugation. CD4 T cells were then isolated by negative selection (Stem Cell, Easy Sep) and resting CD4 T cells were obtained from this population by depleting cells expressing HLA-DR, CD25 and CD69 (Miltenyi). Resting CD4 T cells were stained in bulk for 1) CD99-FITC (Biolegend), CCR10-APC (BD Biosciences), and Propridium Iodide to distinguish viable cells; 2) CD4-PeCy7 only (Biolegend); or 3) CD45RA-PE (Biolegend) and CD45RO-APC (Biolegend). These three populations of cells were sorted independently on the MoFlo.
Song N., Sengupta S., Khoruzhenko S., Welsh R.A., Kim A., Kumar M.R., Sønder S.U., Sidhom J.W., Zhang H., Jie C., Siliciano R.F, & Sadegh-Nasseri S. (2020). Multiple genetic programs contribute to CD4 T cell memory differentiation and longevity by maintaining T cell quiescence. Cellular immunology, 357, 104210.
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4

Multiparametric Flow Cytometry for Immune Profiling

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Surface marker expression on DCs was assessed via flow cytometry by staining the cells with α-HLA-DR APC, Fixable Viability Dye eFluor506, α-CD1a BV421, α-CD86 PE (eBioscience), α-CD14 PerCP-Cy5.5 (BD Bioscience) and α-ILT3 PerCP-Cy5.5 (Biolegend). Samples were subsequently acquired using a FACS Canto II flow cytometer (BD Biosciences). The shown MFI data were collected after exclusion of doublets and dead cells.
Surface marker expression on PBMCs was assessed via flow cytometry using a 13-color flow-cytometry panel generated by using the Optimized Multicolor Immunofluorescence Panel-030 (OMIP-030)34 as a reference: CD4 BV510, CD127 AlexaFluor 700, CD8a PE-Cy5, CD3 PerCP-Cy5.5, CD45RA BV605, CD25 PE, CD20 APC-Cy7, CD194 PE-Cy7, CD197 PE/Dazzle594 (Biolegend), CCR10 APC (BD Biosciences), Ki-67 BV650, CD196 BB151, CD183 BV421 (BD Horizon) Fixable Viability Dye APC-Cy7 (eBioscience). Samples were acquired using a Cytoflex S flow cytometer (Beckman Coulter) and then analyzed using the gating strategy summarized in ESI Fig. 5.†
Michelini S., Barbero F., Prinelli A., Steiner P., Weiss R., Verwanger T., Andosch A., Lütz-Meindl U., Puntes V.F., Drobne D., Duschl A, & Horejs-Hoeck J. (2021). Gold nanoparticles (AuNPs) impair LPS-driven immune responses by promoting a tolerogenic-like dendritic cell phenotype with altered endosomal structures. Nanoscale, 13(16), 7648-7666.
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5

Multiparametric Flow Cytometry Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse FITC-CD4 and eFluor 605-CD44 [BD-Pharmingen]; KJ1.26 anti-TCR (Caltag Laboratories), Brilliant Violet 570-CD4 and Alexa Fluor 700-CD44 were from [Biolegend], FITC-Klotho,PE-Cy7-CD99, PE-Cy5-VDR, and PE-Cy7-CNR2 were from [Bioss]; APC-CCR10 and APC-ITGA3 were from [R&D Systems]; Fixable viability dye eFluor 780 was from [eBioscience]. Antibodies for human PBMC staining were as follows: from Biolegend, CD99-FITC,CD69-BV421 and CD69-APC , CD45R0-APC and CD45R0-BV421, CD3-AF700, CD4-Pe/Cy7, CD45RA-PE, Itga3 (CD49c)-PE, IL-7R-BV510, CD25-APC, HLA-DR-APC. From BD Biosciences, CCR10-APC and CCR10-PerCP Cy5.5. and viability dye eFluor 780 as above. Relevant mouse anti human isotype antibodies to IgG1k or IgG2ak were utilized for the following fluorophores: BV421, BV510, FITC, PE, and APC for both isotypes (Biolegend).
Song N., Sengupta S., Khoruzhenko S., Welsh R.A., Kim A., Kumar M.R., Sønder S.U., Sidhom J.W., Zhang H., Jie C., Siliciano R.F, & Sadegh-Nasseri S. (2020). Multiple genetic programs contribute to CD4 T cell memory differentiation and longevity by maintaining T cell quiescence. Cellular immunology, 357, 104210.
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