Example 4
Next Applicants evaluated 37-cycle PCR for all targets to reduce or eliminate contamination from blood matrix bottles. Three types of bottles were tested (Bactec Pediatric Plus/F, Bactec Aerobic Plus/F, Bactec Anaerobic Lytic/10) with and without blood.
Surprisingly, when PCR cycles are reduced from 40 to 37, most blood matrix contamination is eliminated.