To evaluate fatty acid composition of oils obtained from A. vulgare, C. nucifera, and E. guineensis, alkaline trans-methylation of fatty acids was carried out as described by Raes et al.100 (link). Analysis of methyl esters was performed using gas chromatography (GC) with the HP 6890 chromatograph (Agilent Technologies, Inc., Santa Clara, CA, USA), equipped with a 60 m DB-23 capillary column (60 m × 0.25 mm × 0.25 μm) and a flame-ionization detector (FID); split injections were performed using an Agilent autosampler. A total of 1 µL of standards in hexane were injected in split mode (1:40 ratio) into the injector, heated to 230 °C. The column temperature was initially set at 120 °C for 6 min then programmed to 170 °C at a rate of 15 °C/min. The temperate gradient was further configured to 210 °C at the rate of 3 °C/min and held for 13.5 min. Finally, the temperature was programmed to 230 °C at the rate of 40 °C/min and held for 7 min. Nitrogen was used as the carrier gas, at a flow rate of 0.8 mL/min. Supelco 37 Component FAME Mix, PUFA 1, PUFA 2, PUFA 3, trans-vaccenic acid, and a mixture of conjugated isomers of linoleic acid (Sigma-Aldrich, Prague, CZ) were used as standards. Fatty acids were identified based on retention times with respect to standards.
60 m db 23 capillary column
Manufactured by Agilent Technologies
Sourced in United States
The 60 m DB-23 capillary column is a gas chromatography column designed for the separation and analysis of fatty acid methyl esters (FAMEs). It features a length of 60 meters and a stationary phase of cyanopropyl polysiloxane. This column is intended for use in gas chromatography applications where the separation and identification of FAMEs is required.
Automatically generated - may contain errors
Lab products found in correlation
Hp 6890 chromatograph, by Agilent Technologies (1 mentions)
Pufa 1, by Merck Group (1 mentions)
Pufa 3, by Merck Group (1 mentions)
Agilent autosampler, by Agilent Technologies (1 mentions)
Pufa2, by Merck Group (1 mentions)
Trans vaccenic acid, by Merck Group (1 mentions)
Supelco 37 component fame mix, by Merck Group (1 mentions)
Linoleic acid, by Merck Group (1 mentions)
3 protocols using 60 m db 23 capillary column
1
Fatty Acid Composition Analysis of Oils
2
Fatty Acid Composition Analysis of Mushrooms
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fatty acid composition analysis was performed at Lipid Analytical Laboratories Inc, Guelph, Ontario, Canada. The fatty acid compositions of all four of the studied mushrooms were determined according to the previously reported lipid extraction method [14 (link)], in the presence of known amounts of fatty acid methyl esters (FAMEs) as internal standards (Nu-Chek Prep Inc., Elysian, MN, USA) for total fatty acid analyses. An aliquot of the fatty acid extracted was used to form FAMEs through transmethylation following a previously published method [15 (link)]. The FAMEs were prepared using boron trichloride in methanol and the methylation tubes were heated to 95 °C in a boiling water bath. The FAMEs were analyzed on an Agilent 7890B GLC (Santa Clara, CA, USA) equipped with a flame ionization detector (FID) and a 60 m DB-23 capillary column (0.32 mm internal diameter) [16 (link)]. FAMEs were identified using a standard mixture (qualitative and quantitative) with 49 known fatty acid components for verification, which were obtained from the American Oil Chemists Society (Champaign, IL, USA) and Sigma-Aldrich (St. Louis, MO, USA).
3
Phospholipid Fatty Acid Composition Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Analysis of FA composition of the phospholipid fractions of proximal jejunum and ileum tissues was performed by Lipid Analytical Laboratories Inc. (Guelph, Canada). Lipids, including phospholipids, were extracted from the samples according to the method of Bligh and Dyer (1959) (link). Extracts were dried under nitrogen, re-solubilized in 200 µL of dichloromethane, and separated by solid-phase extraction on a silica gel 60 thin layer chromatography (TLC) plate (Merck; 5721-7). The TLC plates were developed in the mobile phase (heptane: isopropyl ether: acetic acid, 60:40:3 vol/vol/ vol; Holub et al., 2011) (link). After the solvent had moved 80% up the plate, the plates were removed from the TLC tank, allowed to dry for 5 min in the fume hood, and sprayed with 8-anilino-1-naphthalene sulfonic acid in methanol to identify bands of interest based on radio frequency comparisons to appropriate standards by using long-wave UV. Using a single-edged razor blade, bands of interest were scraped off into screw-top glass test tubes. The FA methyl esters were prepared with boron trichloride in methanol. The methylation tubes were heated by hot plate for 50 min at 95°C. The resulting FA methyl esters were then analyzed on an Agilent 7890B gas-liquid chromatograph with a 60-m DB-23 capillary column (0.32-mm internal diameter; Morrison and Smith, 1964) (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!