Tcs sp8 imaging system

Manufactured by Leica

Sourced in China

The Leica TCS SP8 imaging system is a high-performance confocal microscope designed for advanced imaging applications. It provides high-resolution, multi-dimensional imaging capabilities, allowing users to capture detailed images of biological samples.

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Lab products found in correlation

Dapi fluoromount, by Beyotime (2 mentions) 7 aminoactinomycin d 7 aad, by Thermo Fisher Scientific (1 mentions) 4 6 diamindino 2 phenylindole dapi, by Vector Laboratories (1 mentions)

Close spelling variants of this product

TCS SP8 (3513 citations) TCS SP8 system (69 citations) SP8 system (38 citations) TCS SP8 confocal imaging system (12 citations) TCS SP8 live cell imaging system (4 citations) TCS SP8 MP confocal imaging system (4 citations) TCS SP8 X confocal spectral microscope imaging system (4 citations) TCS-SP8 imaging (2 citations) TCS-SP8 LSM confocal imaging system (2 citations) TCS SP8 MP multiphoton imaging system (2 citations)

7 protocols using tcs sp8 imaging system

Decalcified Stone Imaging and Tissue Staining

Cited 1 time

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Decalcified stones were dehydrated through a series of graded ethanol
concentrations and then embedded in paraffin. Paraffinized and unstained
(label-free) sections of stones were imaged by fluorescence on a Leica TCS SP8
imaging system; and multiphoton excitation at 910 nm for second harmonic
generation (SHG) imaging was used for collagen detection (Ferkowicz et al., 2021 (link)). Subsequent staining of human
renal tissue and demineralized stone sections were deparaffinized, permeabilized
with 0.05% Triton X-100 and stained using 7-aminoactinomycin D (7-AAD) (Thermo
Fisher Scientific, Itasca, Illinois, USA) or
4’,6-diamindino-2-phenylindole (DAPI) (Vector Laboratories, Burlingame,
CA, USA) for DNA detection. Additional sections were stained by routine
hematoxylin & eosin (H&E) methods. Observations were all done in
triplicate, except where noted.

Canela V.H., Bledsoe S.B., Worcester E.M., Lingeman J.E., El-Achkar T.M, & Williams JC J.r. (2021). Collagen fibrils and cell nuclei are entrapped within Randall’s plaques but not in CaOx matrix overgrowth: A microscopic inquiry into Randall’s plaque stone pathogenesis. Anatomical record (Hoboken, N.J. : 2007), 305(7), 1701-1711.

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Visualizing SARS-CoV-2 Protein Localization

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To visualize the ectopic localization of the SARS-CoV-2 viral proteins, hiPSC derived cardiomyocytes were infected with adenoviruses encoding tubulin-GFP (ex/em 484/507 nm; MOI 5) and Sec61 mCherry (Vector BioLabs, ex/em 587/610 nm; MOI 5). After 24 h, the cells were infected with SARS-CoV-2 virus (MOI 1) for 24 h and fixed with 10% neutral buffered formalin and imaged on a Leica TCS SP8 imaging system using a 100X oil objective. The phenotypic implications of the SARS-CoV-2 were analyzed using the Line Scan analysis, Leica Application Suite X.

Ramachandran K., Maity S., Muthukumar A.R., Kandala S., Tomar D., Abd El-Aziz T.M., Allen C., Sun Y., Venkatesan M., Madaris T.R., Chiem K., Truitt R., Vishnu N., Aune G., Anderson A., Martinez-Sobrido L., Yang W., Stockand J.D., Singh B.B., Srikantan S., Reeves W.B, & Madesh M. (2022). SARS-CoV-2 infection enhances mitochondrial PTP complex activity to perturb cardiac energetics. iScience, 25(1), 103722.

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Arabidopsis Transformation and Heat Stress

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Wide-type Arabidopsis was transformed with Agrobacterium tumefaciens strain GV3101 carrying GFP-SR30 constructs by the floral dipping method. In addition, 8-day-old transgenic T2 seedlings grown on MS medium were covered by aluminum foil and were subjected to 39 °C for 6 h. Control plants were covered by aluminum foil and were put under normal growth conditions. After 6 h, seedling leaves were examined by a confocal microscope (Leica TCS SP8 imaging system).

Zhang D., Chen M.X., Muhammad Aslam M., Liu Y.G, & Zhang J. (2023). Global Analysis of Dark- and Heat-Regulated Alternative Splicing in Arabidopsis. International Journal of Molecular Sciences, 24(6), 5299.

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Confocal Imaging of Live Cells

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The imaging experiments were conducted with xyzt shooting mode under Leica TCS SP8 imaging system with following set: λ_ex = 488 nm, λ_em = 500‒640 nm, a scan speed of 100 Hz, a pixel resolution of 45.1 nm × 45.1 nm, a z-step of 300 nm, a total of 40 sets of 3D imaging, about 2000 confocal images were taken. In addition, a microscopy-suited incubator was employed to control the temperature (37 °C) and CO₂ concentration (5%).

Zhou R., Wang C., Liang X., Liu F., Sun P., Yan X., Jia X., Liu X., Wang Y, & Lu G. (2023). A new organic molecular probe as a powerful tool for fluorescence imaging and biological study of lipid droplets. Theranostics, 13(1), 95-105.

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Fluorescent Imaging of Transfected Cells

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Transfected HEK-293T cells grown in Cellvis plastic dishes were first fixed with 4% paraformaldehyde at room temperature for 30 min, then stained with DiD or ActinRed (diluted in 0.5% Triton X-100 PBS) for ∼30 min after PBS rinses. After washing off the fluorescent dye with PBS, fixed cells were embedded in DAPI-Fluoromount (Beyotime, Shanghai, China) and characterized with a Leica TCS SP8 imaging system in fluorescence imaging mode. The resulting images were analyzed with ImageJ (Java 1.8.0_172/1.52b) (Schindelin et al., 2012 (link)).

Song J., Liu C., Li B., Liu L., Zeng L., Ye Z., Mao T., Wu W, & Hu B. (2022). Tunable Cellular Localization and Extensive Cytoskeleton-Interplay of Reflectins. Frontiers in Cell and Developmental Biology, 10, 862011.

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Quantifying Autophagy in SARS-CoV-2 Infection

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To evaluate the suicidal effect of cells in response to the SARS-CoV-2 infection, hiPSC derived cardiomyocytes were infected with LC3 GFP/RFP tandem adenovirus (GFP ex/em 488/510 nm; RFP ex/em 552/584 nm; MOI 5). After 24 h, the cells were infected with SARS-CoV-2 (MOI 1) for 24 h and fixed with 10% neutral buffered formalin. The fixed cells were imaged on a Leica TCS SP8 imaging system using a 100x oil objective. The number of vesicular puncta were counted and plotted using GraphPad Prism version 8.

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Fluorescent Imaging of Transfected HEK Cells

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Transfected HEK-293T cells grown in Cellvis plastic dishes were firstly fixed with 4% paraformaldehyde at room temperatures for 30 min and then stained with DiD (diluted in 0.5% Triton X-100 PBS) for ∼30 min after PBS rinses. Fixed cells were embedded in DAPI-Fluoromount (Beyotime, Shanghai, China) after washing off the fluorescent dye with PBS, and characterized with a Leica TCS SP8 imaging system in fluorescence imaging mode. 405 Diode laser was used for DAPI detection; 488 Argon was used for GFP detection; DPSS 561 was used for mcherry detection; HeNeB 633 was used for DiD detection. The resulting images were analyzed with ImageJ (Java 1.8.0_172/1.52b) (Schindelin et al., 2012).

Song J., Liu C., Li B., Liu L., Zeng L., Ye Z., Wu W., Zhu L, & Hu B. (2023). Synthetic peptides for the precise transportation of proteins of interests to selectable subcellular areas. Frontiers in Bioengineering and Biotechnology, 11, 1062769.

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