Lutein (purity > 90%), alcalase (enzyme activity > 200 U/mg), bile salts, and molecular weight standards including bacitracin, aprotinin, myohemoglobin, bovine serum albumin, and thyroglobulin were purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). Soybeans were bought from Fengyuan Zhongye Co., Ltd. (Lianyungang, China). Pepsin (enzyme activity > 2500 U/mg), trypsin (enzyme activity > 2500 U/mg), 8-aniline-1-naphthalensulfonic acid (ANS), and trinitrobenzenesulfonic acid (TNBS) were purchased from Sigma-Aldrich Co., (St. Louis, MO, USA). All other reagents and solvents were of at least analytical grade.
Bovine serum albumin (bsa)
Manufactured by Yuanye Bio-Technology
Sourced in China
Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood. It is a protein that serves as a stabilizer, blocking agent, and nutrient supplement in various biological and biochemical applications.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin, by Merck Group (2 mentions)
Bacitracin, by Yuanye Bio-Technology (2 mentions)
Aprotinin, by Yuanye Bio-Technology (2 mentions)
Alcalase, by Yuanye Bio-Technology (2 mentions)
Freund s adjuvant, by Merck Group (2 mentions)
Pepsin, by Merck Group (1 mentions)
Lutein, by Yuanye Bio-Technology (1 mentions)
Trinitrobenzene sulfonic acid tnbs, by Merck Group (1 mentions)
Bicinchoninic acid bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
1 1 diphenyl 2 picrylhydrazyl dpph, by Merck Group (1 mentions)
α amylase, by Merck Group (1 mentions)
Pancreatin, by Yuanye Bio-Technology (1 mentions)
Pepsin, by Solarbio (1 mentions)
Serine, by Solarbio (1 mentions)
Fluorescein isothiocyanate (fitc), by Yuanye Bio-Technology (1 mentions)
N hydroxysuccinimide, by Yuanye Bio-Technology (1 mentions)
Nile red, by Yuanye Bio-Technology (1 mentions)
Sds page electrophoresis solution, by Beyotime (1 mentions)
Transglutaminase, by Yuanye Bio-Technology (1 mentions)
Gallic acid, by Yuanye Bio-Technology (1 mentions)
20 protocols using bovine serum albumin (bsa)
1
Soy Protein Extraction and Characterization
2
Quantitative Pea Protein Characterization
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Pea protein isolate (PPI, 90%) was purchased from Xi'an Zelong Biotech Co., Ltd. (Xian, China). Bovine serum albumin (BSA, 20 mg/mL) was purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China). Bicinchoninic acid (BCA) protein assay kit was obtained from Thermo Fisher Scientific (USA). 8-Aniline-1-naphthalene sulfonic acid (ANS) was obtained from Aladdin Biochemical Technology Co., Ltd. (Beijing, China). Corn oil was purchased from Luhua Group Co., Ltd. (Shandong, China).
3
Soy Flour Protein Extraction and Characterization
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Defatted soy flour with low protein denaturation was obtained from Pingdingshan Jinjing Biological Technology Co., Ltd. (Zhengzhou, Henan, China). Alcalase (200 U mg−1) was purchased from Shanghai Yuanye Biological Technology Co., Ltd. (Shanghai, China). C3G (purity ≥ 98%) was purchased from Nanjing Spring and Autumn Biotech Co., Ltd. (Nanjing, Jiangsu, China). 1-anilinonaphthalene-8-sulfonic acid (ANS) was obtained from Aladdin Co., Ltd. (Shanghai, China). 2,2′-azino-di-(3-ethylbenzthiazoline sulfonic acid) diammonium salt (ABTS) was obtained from Fluorochem Co., Ltd. (Hadfield, Derbyshire, UK), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Molecular weight standards including cytochrome C, aprotinin, bacitracin, Gly−Gly−Tyr−Arg, Gly−Gly−Gly, carbonic anhydrase, bovine serum albumin, alcohol dehydrogenase, β-amylase and apoferritin were all purchased from Shanghai Yuanye Biological Technology Co., Ltd. (Shanghai, China). All other reagents were of analytical grade and were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China) unless otherwise noted.
4
Enzymatic Digestion of Proteins
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Water used in all the experiments was obtained from a reverse osmosis water purification system. Trypsin (1,000–2,000 U/mg) and α-amylase (≥5,000 U/g) from the porcine pancreas were purchased from Sigma-Aldrich, Switzerland. Pepsin (≥250 U/mg) from porcine gastric mucosa, serine, and artificial saliva (pH = 6.8) were obtained from Solarbio, Beijing, CN. Pancreatin (≥4,000 U/g), o-phthalaldehyde, and bovine serum albumin were bought from Yuanye, Shanghai, CN. Ethanol and petroleum ether were purchased from Aladdin, Shanghai, CN. All the chemical reagents used were of analytical grade.
5
Antimicrobial Peptide MOp2 Nanoparticles
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The antimicrobial peptide MOp2 was obtained from Moringa oleifera seeds (purity>95%) (minimal inhibitory concentration against S. aureus = 2 mg/mL) which was synthesized by Guoping Pharmaceutical Co. Ltd. (Anhui, China) (Fig. S1 ). Food grade chitosan was purchased from Yili Biotechnology Co. Ltd. (Henan, China). Ellmans reagent, 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide hydrochloride (EDC, purity>97%), N-hydroxysuccinimide (NHS, purity>98%), bovine serum albumin (purity>98%), Nile red (purity>98%), and fluorescein isothiocyanate (FITC, purity>90%) were purchased from Yuanye Biotechnology Co. Ltd. (Shanghai, China). Sodium tripolyphosphate (STPP, purity>98%) was purchased from Aladdin Biochemical Technology Co. Ltd. (Shanghai, China). All other chemicals were of analytical grade.
The target strains S. aureus CICC 10384 and E. coli CICC 10003 were procured from the China Center of Industrial Culture Collection. These strains were cultured in Luria-Bertani (LB) liquid medium, passaged to the second generation and stored at −80 °C for use.
The target strains S. aureus CICC 10384 and E. coli CICC 10003 were procured from the China Center of Industrial Culture Collection. These strains were cultured in Luria-Bertani (LB) liquid medium, passaged to the second generation and stored at −80 °C for use.
6
Tilapia Collagen Peptide Enrichment
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Fresh tilapia (average length: 30 ± 5 cm, weight: 1300 ± 20 g) was purchased from Dongfeng Market (Zhanjiang, China). Fish collagen peptide was purchased from Ruikanglai Technology Co., Ltd. (Nanjing, China); glucosamine hydrochloride was purchased from Cohen Biotechnology Co., Ltd. (Yangzhou, China); transglutaminase and bovine serum albumin were purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China); sodium chloride, calcium chloride, and ethanol were purchased from Guanghua Technology Co., Ltd. (Guangdong, China); and SDS–PAGE electrophoresis solution was purchased from Biyuntian Biotechnology Co., Ltd. (Shanghai, China).
7
Extraction and Analysis of Hawthorn Bioactive Compounds
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Fresh hawthorn (variety: Dajinxing) was procured in Nanma Village, Xiaodian District (Taiyuan, China) in early October 2022, with a moisture content of 79%. Citric acid, sodium hydroxide, salicylic acid, ferric chloride, and trichloroacetic acid were purchased from Damao Chemical Reagent Co., Ltd. (Tianjin, China). n-Butanol was obtained from Zhiyuan Chemical Reagent Co., Ltd. (Tianjin, China). Anhydrous ethanol was acquired from Yongda Chemical Reagent Co., Ltd. (Tianjin, China). Sodium tetraborate was sourced from Beijing Solaibao Biotechnology Co., Ltd. (Beijing, China). Chloroform and sulfuric acid were provided by the laboratory. Potassium ferrocyanide was procured from Tianjin Fengchuan Chemical Reagent Technology Co., Ltd. (Tianjin, China). Coomassie Brilliant Blue G250, bovine serum albumin, anthrone, gallic acid and ascorbic acid were purchased from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China). Except for gallic acid, which was used as a standard, all other chemical reagents were of analytical grade.
8
Optimized Immunoassay Protocol
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BPLX MAG COOH Beads #26, BPLX MAG COOH Beads #45, Bio-Plex Amine Coupling Kits and ProteOn Amine Coupling Kits all were purchased from BioRad (Hercules, CA). Fluorescein-isothiocyanate (FITC)-conjugated AffiniPure goat anti-mouse IgG (1.5 mg/ml stock), AffiniPure goat anti-mouse IgM (l chain-specific IgG; 2.3 mg/ml stock), FITC-AffiniPure goat anti-mouse IgG (H þ L), and horseradish peroxidase (HRP)conjugated goat anti-mouse IgG (2.0 mg/ml stock) were all obtained from Jackson ImmunoResearch (West Grove, PA). Purified mouse anti-KLH polyclonal IgG (0.4 mg/ml stock) was bought from Aibixin Biotechnology(Shanghai, China). Other reagents purchased were as follows: KLH (Sigma, St. Louis, MO); Tween-20 (Aladdin, Shanghai, China); phosphate-buffered saline (PBS [pH 7.4]; Boster Biological Technology, Wuhan, China); bovine serum albumin (BSA; Yuanye Biotechnology, Shanghai, China); and, cyclophosphamide (CYP: Jiangsu Baxter Oncology Gmbh; Kantstr, Halle, Germany).
9
HTRF® KinEASE-TK Assay for EGFR Kinase Activity
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HTRF® KinEASE-TK assay kit was obtained from Cisbio (Codolet, France). EGFR protein (GST-tagged) was obtained from Thermo Fisher Scientific (Carlsbad, CA, USA). Dulbecco's modified Eagle's medium (DMEM, low glucose), penicillin and streptomycin, trypsin, and phosphate buffer saline (PBS) were obtained from Gibco (Paisley, UK). Fetal bovine serum (FBS) was obtained from Zhejiang Tianhang Biotechnology Co., Ltd. (Zhejiang, Hangzhou, China). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was obtained from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). 20(S)-Rh2 and bovine serum albumin (BSA) were obtained from Yuanye Biotech Co., Ltd. (Shanghai, China). Dimethyl sulfoxide (DMSO) was obtained from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). Ethylenediamine tetraacetic acid (EDTA) was obtained from Sigma-Aldrich (MO, USA). Goat anti-rat IgG secondary antibody was obtained from Sino Biological Inc. (Beijing, China). Antibody against GAPDH was obtained from Gene Tex (Irvine, CA, USA). Other primary antibodies were all obtained from Abcam (Cambridge, MA, USA). All other chemical reagents were of analytical grade.
10
Pecan Nut Protein Extraction Protocol
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The pecan nuts (Carya illinoinensis (Wangenh.) K. Koch, Pawnee variety) were purchased from Anhui Jiayu Agricultural Co., Ltd., Anhui, China. Alkaline proteinase (powder, 200 U/mg, CAS number 9014-01-1) from Bacillus Subtilis, bovine serum albumin (BSA), 1-Anilino-8-naphthalene-sulfonate (ANS) and 5, 5′-dithio-bis-2-nitrobenzoic acid (DTNB) were obtained from Shanghai Yuanye Biotechnology Co., Ltd., Shanghai, China. Pecan nuts and chemicals were stored at 4 °C.
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