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Accutrend lactate analyzer

Manufactured by Roche
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Sourced in Germany

The Accutrend Lactate analyzer is a compact, portable device designed for the measurement of lactate levels in blood. It provides quick and accurate results, making it a useful tool for monitoring and managing various health conditions. The analyzer utilizes an electrochemical detection method to determine the lactate concentration in a small blood sample.

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Lab products found in correlation

Fluorescent microscope, by Olympus (2 mentions) 2 nbdg, by Thermo Fisher Scientific (2 mentions) Freestyle glucose meter, by Abbott (2 mentions) Facscanto flow cytometer, by BD (2 mentions) Cobas 8000, by Roche (1 mentions) Poch 100i automated hematology analyzer, by Sysmex (1 mentions) Glucose uptake colorimetric assay kit, by Abcam (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Celltiter glo luminescent cell viability assay kit, by Promega (1 mentions) Amplex red glucose assay kit, by Thermo Fisher Scientific (1 mentions) Oxytherm, by Hansatech (1 mentions)

Close spelling variants of this product

Accutrend (23 citations) Accutrend Lactate (13 citations)

12 protocols using accutrend lactate analyzer

1

Lactate Production in Cell Culture

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To measure lactate production, cells that were 80% confluent were replenished with fresh medium. Aliquots of the medium were removed at the indicated time points (24 or 48 h) for measurement of lactate, using an Accutrend lactate analyzer (Roche). At each time point, cell numbers were also counted for normalization of lactate generation.
Redis R.S., Vela L.E., Lu W., de Oliveira J.F., Ivan C., Rodriguez-Aguayo C., Adamoski D., Pasculli B., Taguchi A., Chen Y., Fernandez A.F., Valledor L., Van Roosbroeck K., Chang S., Shah M., Kinnebrew G., Han L., Atlasi Y., Cheung L.H., Huang G.Y., Monroig P., Ramirez M.S., Ivkovic T.C., Van L., Ling H., Gafà R., Kapitanovic S., Lanza G., Bankson J.A., Huang P., Lai S.Y., Bast R.C., Rosenblum M.G., Radovich M., Ivan M., Bartholomeusz G., Liang H., Fraga M.F., Widger W.R., Hanash S., Berindan-Neagoe I., Lopez-Berestein G., Ambrosio A.L., Gomes Dias S.M, & Calin G.A. (2016). Allele-specific reprogramming of cancer metabolism by the long non-coding RNA, CCAT2. Molecular cell, 61(4), 520-534.
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2

Comprehensive Blood Biomarker Analysis Protocol

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Blood samples were collected during each session within 10 minutes of arrival and prior to each ultrasound. Samples were drawn from an antecubital vein into a dried, heparinized or EDTA tube, depending on the analysis being performed. Both tubes were immediately centrifuged for 10 minutes (3500 RPM). Because it was not possible to carry out all analyses on the same day using point-of-care technology, plasma and serum samples were frozen at −80°C within 20 minutes of blood collection and stored for later analyses of muscle injury markers and biochemical parameters (Table 1). Lactate was measured directly using an Accutrend Lactate Analyzer (Roche Diagnostics, Manheim, Germany). All hematology parameters (hemoglobin, red blood cells, white blood cells) were analyzed directly using a pocH-100i™ Automated Hematology Analyzer (Sysmex, Villepinte, France). Cobas 8000 and Cobas 6000 Modular Analyzers (Roche Diagnostics, Manheim, Germany) were used to perform serial CK, CKMB, hsTnT, NT-proBNP, MYO, and hsCRP measurements, as well as serial electrolyte, protein and hepatic and renal biomarker measurements.
Andonian P., Viallon M., Le Goff C., de Bourguignon C., Tourel C., Morel J., Giardini G., Gergelé L., Millet G.P, & Croisille P. (2016). Shear-Wave Elastography Assessments of Quadriceps Stiffness Changes prior to, during and after Prolonged Exercise: A Longitudinal Study during an Extreme Mountain Ultra-Marathon. PLoS ONE, 11(8), e0161855.
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3

Metabolic Profiling of Colorectal Cancer Cells

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Glucose consumption was assessed with Glucose Uptake Colorimetric Assay Kit (Catalog # K676; Biovision). Briefly, 1.5 × 103 ALDH+ CRC cells were infected with adenoviruses and seeded at each well in a 96-well plate. Forty eight hrs later, the cells were starved by pre-incubating with 100 μl Krebs-Ringer-Phosphate-HEPES (KRPH) buffer containing 2% BSA for 40 min, followed by addition of 2-DG and 20 min incubation. Absorbance was measured at 412 nm in a microplate reader and glucose uptake in these cells was determined according to the standard curves.
To quantify the lactate produced by CRC cells, ALDH+ cells were transfected and seeded at the density of 5 × 105 cells per well at 6-well plate. The cells were washed with PBS 48 hrs later and incubated with fresh medium. After 2 hrs, aliquots of the culture medium were harvested for analysis of lactic acids using an Accutrend lactate analyzer with a linear range of standard lactate concentrations (Roche, Germany).
PDH activity was tested following the procedures described elsewhere [31 (link)].
Wang J., Lei Z.J., Guo Y., Wang T., Qin Z.Y., Xiao H.L., Fan L.L., Chen D.F., Bian X.W., Liu J, & Wang B. (2015). miRNA-regulated delivery of lincRNA-p21 suppresses β-catenin signaling and tumorigenicity of colorectal cancer stem cells. Oncotarget, 6(35), 37852-37870.
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4

Quantifying Glucose Uptake and Lactate Production

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For glucose uptake assay, cells were seeded in 60 mm plates. After 24 h, cells were refreshed with full serum (10% FBS) and glucose-free DMEM (Gibco, Cat# 11966025), while the complete DMEM high glucose medium served as control. After 8 h, cells were treated with 50 μM 2-NBDG for 30 min and glucose uptake rate was quantified by FACS analysis. For lactate production assay, cells were plated in 24-well plate and cultured overnight in low serum medium (0.1% FBS). After serum starvation, cells were treated with or without EGF (100 ng/ml) for overnight. Culture medium was then transferred from each well to Eppendorf tube. Lactate concentration was determined by using lactate test strips and reading under Accutrend Lactate analyzer (Roche). Next, cells were harvested, stained with trypan blue and viable cell numbers were counted directly under the microscope using hemocytometer. The lactate production was determined by lactate concentration/cells and normalized with the rate detected in the control group without EGF.
Han F., Li C.F., Cai Z., Zhang X., Jin G., Zhang W.N., Xu C., Wang C.Y., Morrow J., Zhang S., Xu D., Wang G, & Lin H.K. (2018). The critical role of AMPK in driving Akt activation under stress, tumorigenesis and drug resistance. Nature Communications, 9, 4728.
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5

Quantifying Glucose Metabolism in 14-3-3σ-Deficient Cells

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In these assays, HCT116 WT, HCT116 14-3-3σ−/−, HCT116 Luciferase shRNA, HCT116 14-3-3σ shRNA #419, HCT116 14-3-3σ shRNA #483, HCT116 14-3-3σ−/− TetR 14-3-3σ, MDA-MB-231 TetR 14-3-3σ cells were plated with the same number (200,000 cells/well in 6-well plates). After 48 hours, medium was collected for measuring lactate and glucose concentrations. Lactate concentrations were measured with an Accutrend Lactate Analyzer (Roche Diagnostics). Glucose consumption was determined by measuring the difference in glucose concentrations at the beginning and the end of experiments. Glucose level was quantified by using a Freestyle Glucose Meter (Abbott Laboratories). The final live cell numbers were counted using hemocytometer and Trypan Blue staining. Lactate production and glucose consumption were normalized to final the final number of live cell and culture time. Glucose uptake in cells was quantified by using (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG)), a green fluorescent glucose analog (Molecular Probes, Invitrogen). Experimental cells were incubated in glucose-free DMEM with 10% FBS containing 120 μM 2-NBDG for time intervals ranging from 0 to 3 hours. 2-NBDG uptake was analyzed by using a fluorescent microscope (Olympus) and a FACS Canto flow cytometer (BD Biosciences). Flow cytometry data was analyzed using a FlowJo X software (FlowJo).
Phan L., Chou P.C., Velazquez-Torres G., Samudio I., Parreno K., Huang Y., Tseng C., Vu T., Gully C., Su C.H., Wang E., Chen J., Choi H.H., Fuentes-Mattei E., Shin J.H., Shiang C., Grabiner B., Blonska M., Skerl S., Shao Y., Cody D., Delacerda J., Kingsley C., Webb D., Carlock C., Zhou Z., Hsieh Y.C., Lee J., Elliott A., Ramirez M., Bankson J., Hazle J., Wang Y., Li L., Weng S., Rizk N., Wen Y.Y., Lin X., Wang H., Wang H., Zhang A., Xia X., Wu Y., Habra M., Yang W., Pusztai L., Yeung S.C, & Lee M.H. (2015). The cell cycle regulator 14-3-3σ opposes and reverses cancer metabolic reprogramming. Nature communications, 6, 7530.
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6

Metabolic Profiling of Cultured Cells

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For measuring oxygen consumption (OCR), cells were trypsinized and resuspended in 1 ml fresh culture medium pre-equilibrated with 21% oxygen at 37 °C; the cells were then transferred to the sealed respiration chamber of a Clark-type oxygen measuring system (Oxytherm, Hansatech Instruments) Cellular glucose uptake was determined by using the Amplex Red Glucose Assay Kit (Invitrogen) according to the manufacturer's instructions. Briefly, cells in exponential growth phase were washed and incubated in fresh medium. Medium was collected and diluted 1:400 in water. Glucose uptake was determined by the difference in glucose concentration of each sample compared with that of the control medium without cells. To measure lactate production, cells with 80% confluency were replenished with fresh medium. Aliquots of the medium were removed after 12 h for measurement of lactate with an Accutrend lactate analyzer (Roche). Cells were also counted for normalization of glucose uptake and lactate generation. Cellular ATP contents were measured by using the CellTiter-Glo Luminescent Cell Viability Assay kit (Promega) according to the manufacturer's recommendations.
Ju H.Q., Ying H., Tian T., Ling J., Fu J., Lu Y., Wu M., Yang L., Achreja A., Chen G., Zhuang Z., Wang H., Nagrath D., Yao J., Hung M.C., DePinho R.A., Huang P., Xu R.H, & Chiao P.J. (2017). Mutant Kras- and p16-regulated NOX4 activation overcomes metabolic checkpoints in development of pancreatic ductal adenocarcinoma. Nature Communications, 8, 14437.
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7

Hypoxia Induces Lactate Production

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Control or H2AX knockdown MDA-MB-231 cells were plated in 12-well dishes and cultured overnight. Next, cells were incubated in normoxic or hypoxic (1% oxygen) conditions for 24 h. The culture medium was removed from the cells and lactate concentration was determined by using lactate test strips and an Accutrend Lactate analyzer (Roche). Next, cells were harvested and viable cells were counted; rate of lactate production was normalized to the rate detected in the control group.
Rezaeian A.H., Li C.F., Wu C.Y., Zhang X., Delacerda J., You M.J., Han F., Cai Z., Jeong Y.S., Jin G., Phan L., Chou P.C., Lee M.H., Hung M.C., Sarbassov D, & Lin H.K. (2016). A hypoxia-responsive TRAF6-ATM-H2A.X signalling axis promotes HIF1α activation, tumorigenesis and metastasis. Nature cell biology, 19(1), 38-51.
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8

Accutrend Lactate Analyzer Protocol

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The Accutrend® Lactate analyzer (formerly known as Accusport portable lactate analyzer; Roche, Boehringer Mannheim, Indianapolis, IN), uses enzymatic determination and reflectance photometry (wavelength 660 nm). The system reads the lactate levels in the plasma portion of whole blood. After sample processing, 20–25 µl of fluid is placed on the lactate test strip. A glass-fiber layer removes red blood cells before fluid continues to the final layer where a reaction takes place in the detector film resulting in a color change. Lactate is measured by reflectance photometry via a colorimetric lactate-oxidase mediator reaction. Results are provided within 60 seconds. The range of measurement for the analyzer is from 0.7 to 27 mmol/L.
de Almeida S.M., Marquie-Beck J., Bhatt A., Letendre S., McCutchan A, & Ellis R. (2014). Portable lactate analyzer for measuring lactate in cerebrospinal fluid (CSF) and plasma – method-comparison evaluations. Arquivos de neuro-psiquiatria, 72(7), 500-505.
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9

Quantifying Glucose Metabolism in 14-3-3σ-Deficient Cells

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In these assays, HCT116 WT, HCT116 14-3-3σ−/−, HCT116 Luciferase shRNA, HCT116 14-3-3σ shRNA #419, HCT116 14-3-3σ shRNA #483, HCT116 14-3-3σ−/− TetR 14-3-3σ, MDA-MB-231 TetR 14-3-3σ cells were plated with the same number (200,000 cells/well in 6-well plates). After 48 hours, medium was collected for measuring lactate and glucose concentrations. Lactate concentrations were measured with an Accutrend Lactate Analyzer (Roche Diagnostics). Glucose consumption was determined by measuring the difference in glucose concentrations at the beginning and the end of experiments. Glucose level was quantified by using a Freestyle Glucose Meter (Abbott Laboratories). The final live cell numbers were counted using hemocytometer and Trypan Blue staining. Lactate production and glucose consumption were normalized to final the final number of live cell and culture time. Glucose uptake in cells was quantified by using (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG)), a green fluorescent glucose analog (Molecular Probes, Invitrogen). Experimental cells were incubated in glucose-free DMEM with 10% FBS containing 120 μM 2-NBDG for time intervals ranging from 0 to 3 hours. 2-NBDG uptake was analyzed by using a fluorescent microscope (Olympus) and a FACS Canto flow cytometer (BD Biosciences). Flow cytometry data was analyzed using a FlowJo X software (FlowJo).
Phan L., Chou P.C., Velazquez-Torres G., Samudio I., Parreno K., Huang Y., Tseng C., Vu T., Gully C., Su C.H., Wang E., Chen J., Choi H.H., Fuentes-Mattei E., Shin J.H., Shiang C., Grabiner B., Blonska M., Skerl S., Shao Y., Cody D., Delacerda J., Kingsley C., Webb D., Carlock C., Zhou Z., Hsieh Y.C., Lee J., Elliott A., Ramirez M., Bankson J., Hazle J., Wang Y., Li L., Weng S., Rizk N., Wen Y.Y., Lin X., Wang H., Wang H., Zhang A., Xia X., Wu Y., Habra M., Yang W., Pusztai L., Yeung S.C, & Lee M.H. (2015). The cell cycle regulator 14-3-3σ opposes and reverses cancer metabolic reprogramming. Nature communications, 6, 7530.
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10

Measuring Lactate Production in Cells

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Cells were seeded in 24‐well plates in triplicate for 24 h. Cells were then refreshed with serum‐free medium for 24 h, followed by treatment with EGF (50 ng/ml) for 9 h. The culture medium was harvested, and lactate concentration was measured using lactate test strips and an Accutrend Lactate analyzer (Roche). Viable cells in each well were counted by the trypan blue dye exclusion method for normalization. The rate of lactate production was determined (lactate production rate = lactate concentration/cells/time) and normalized to the rate determined for the control group.
He J., Li C., Lee H., Shin D., Chern Y., Pereira De Carvalho B, & Chan C. (2021). MIG‐6 is essential for promoting glucose metabolic reprogramming and tumor growth in triple‐negative breast cancer. EMBO Reports, 22(5), e50781.
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