Log In Sign up
The largest database of trusted experimental protocols

Ace2 flag

Manufactured by Merck Group
Visit Supplier

ACE2-FLAG is a laboratory reagent produced by Merck Group. It is a recombinant protein consisting of the human angiotensin-converting enzyme 2 (ACE2) protein with a FLAG tag. The core function of ACE2-FLAG is to enable researchers to study interactions and binding characteristics of the ACE2 protein.

Automatically generated - may contain errors

Lab products found in correlation

Tranit mrna transfection kit, by Mirus Bio (2 mentions) Anti flag fitc, by Merck Group (2 mentions) Alexafluor 647 goat anti human igg, by Southern Biotech (2 mentions) Live dead fixable aqua stain, by Thermo Fisher Scientific (2 mentions) Lsrii fortessa instrument, by BD (2 mentions) Pe anti flag, by BioLegend (1 mentions) Sars cov 2 2019 ncov spike neutralizing antibody, by Sino Biological (1 mentions) Mouse anti sars cov 2 spike antibody, by Sino Biological (1 mentions) Alexa fluor 647 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Transit mrna transfection kit, by Mirus Bio (1 mentions) Facscelesta, by BD (1 mentions)

3 protocols using ace2 flag

1

Transfection and Analysis of SARS-CoV-2 Spike Protein

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293T cells were transiently transfected with mRNA encoding SARS-CoV-2 WT S or S-2P protein using a TranIT mRNA transfection kit (Mirus). After 24 hr, the cells were harvested and resuspended in FACS buffer (1X PBS, 3% FBS, 0.05% sodium azide). To detect surface protein expression, the cells were stained with 10 μg/mL ACE2-FLAG (Sigma) or 10 μg/mL CR302235 (link) in FACS buffer for 30 min on ice. Thereafter, cells were washed twice in FACS buffer and incubated with FITC anti-FLAG (Sigma) or Alexafluor 647 goat anti-human IgG (Southern Biotech) in FACS buffer for 30 min on ice. Live/Dead aqua fixable stain (Invitrogen) were utilized to assess viability. Data acquisition was performed on a BD LSRII Fortessa instrument (BD Biosciences) and analyzed by FlowJo software v10 (Tree Star, Inc.)
Corbett K.S., Edwards D.K., Leist S.R., Abiona O.M., Boyoglu-Barnum S., Gillespie R.A., Himansu S., Schäfer A., Ziwawo C.T., DiPiazza A.T., Dinnon K.H., Elbashir S.M., Shaw C.A., Woods A., Fritch E.J., Martinez D.R., Bock K.W., Minai M., Nagata B.M., Hutchinson G.B., Wu K., Henry C., Bahi K., Garcia-Dominguez D., Ma L., Renzi I., Kong W.P., Schmidt S.D., Wang L., Zhang Y., Phung E., Chang L.A., Loomis R.J., Altaras N.E., Narayanan E., Metkar M., Presnyak V., Liu C., Louder M.K., Shi W., Leung K., Yang E.S., West A., Gully K.L., Stevens L.J., Wang N., Wrapp D., Doria-Rose N.A., Stewart-Jones G., Bennett H., Alvarado G.S., Nason M.C., Ruckwardt T.J., McLellan J.S., Denison M.R., Chappell J.D., Moore I.N., Morabito K.M., Mascola J.R., Baric R.S., Carfi A, & Graham B.S. (2020). SARS-CoV-2 mRNA Vaccine Design Enabled by Prototype Pathogen Preparedness. Nature, 586(7830), 567-571.
+ Open protocol
+ Expand
2

Transfection and Analysis of SARS-CoV-2 Spike Protein

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293T cells were transiently transfected with mRNA encoding SARS-CoV-2 WT S or S-2P protein using a TranIT mRNA transfection kit (Mirus). After 24 hr, the cells were harvested and resuspended in FACS buffer (1X PBS, 3% FBS, 0.05% sodium azide). To detect surface protein expression, the cells were stained with 10 μg/mL ACE2-FLAG (Sigma) or 10 μg/mL CR302235 (link) in FACS buffer for 30 min on ice. Thereafter, cells were washed twice in FACS buffer and incubated with FITC anti-FLAG (Sigma) or Alexafluor 647 goat anti-human IgG (Southern Biotech) in FACS buffer for 30 min on ice. Live/Dead aqua fixable stain (Invitrogen) were utilized to assess viability. Data acquisition was performed on a BD LSRII Fortessa instrument (BD Biosciences) and analyzed by FlowJo software v10 (Tree Star, Inc.)
Corbett K.S., Edwards D.K., Leist S.R., Abiona O.M., Boyoglu-Barnum S., Gillespie R.A., Himansu S., Schäfer A., Ziwawo C.T., DiPiazza A.T., Dinnon K.H., Elbashir S.M., Shaw C.A., Woods A., Fritch E.J., Martinez D.R., Bock K.W., Minai M., Nagata B.M., Hutchinson G.B., Wu K., Henry C., Bahi K., Garcia-Dominguez D., Ma L., Renzi I., Kong W.P., Schmidt S.D., Wang L., Zhang Y., Phung E., Chang L.A., Loomis R.J., Altaras N.E., Narayanan E., Metkar M., Presnyak V., Liu C., Louder M.K., Shi W., Leung K., Yang E.S., West A., Gully K.L., Stevens L.J., Wang N., Wrapp D., Doria-Rose N.A., Stewart-Jones G., Bennett H., Alvarado G.S., Nason M.C., Ruckwardt T.J., McLellan J.S., Denison M.R., Chappell J.D., Moore I.N., Morabito K.M., Mascola J.R., Baric R.S., Carfi A, & Graham B.S. (2020). SARS-CoV-2 mRNA Vaccine Design Enabled by Prototype Pathogen Preparedness. Nature, 586(7830), 567-571.
+ Open protocol
+ Expand
3

Quantifying SARS-CoV-2 Spike Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293T cells were seeded in 6-well plates as 8×10 5 cells/well and transfected with mRNA encoding codonoptimized S-2P/S-6P candidates or codon-optimized Delta Spike/Omicron Spike candidates utilizing TransIT-mRNA Transfection Kit (Mirus) following the manufacturer's instructions. Cells were harvested 24 h post transfection to perform the immunoblotting. Spike proteins in immunoblotting were detected by mouse anti-SARS-CoV-2 spike antibody (Sino Biological, Cell Signaling Technology). To determine surface protein expression of Spike, the transfected cells were harvested 24 h posttransfection and resuspended in FACS buffer (3% FBS, 0.05% sodium azide in DPBS). Cells were stained with SARS-CoV-2 (2019-nCoV) Spike neutralizing antibody (Sino Biological) or 10 μg/ml ACE2-Flag (Sigma) in FACS buffer for 30 min on ice. Cells were washed by FACS buffer twice and incubated with Alexa Fluor 647 donkey anti-rabbit IgG (Invitrogen) or PE anti-Flag (BioLegend) in FACS buffer for 30 min at 4°C in the dark. DAPI (BV421) staining (Miltenyi Biotec) was utilized to isolate live cells followed by washing step. Flow cytometry data acquisition was performed on BD FACSCelesta (BD Biosciences) and analyzed by FlowJo software v10.
Lai C.J., Kim D., Kang S., Li K., Cha I., Sasaki A., Porras J., Xia T, & Jung J.U. (2023). Viral codon optimization on SARS-CoV-2 Spike boosts immunity in the development of COVID-19 mRNA vaccines. Journal of medical virology, 95(10).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!