Hydrogen tetrachloroaurate (III) (≥99.9%) (Catalogue no. 50790), sodium citrate tribasic dehydrate (≥99.0%) (Catalogue no. 54641), hyaluronic acid (Molecular weight- 1.5 to 1.8 × 106 Da; catalogue no. 53747), polyethylene glycol (average Mn 400; catalogue no. 202398), 1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (catalogue no. E7750), and N-hydroxysuccinimide (NHS) (catalogue no. 130672) were purchased from Sigma-Aldrich and used without further purification. Dulbecco’s Modified Eagle’s medium (DMEM; catalogue no.11995065), Fetal Bovine Serum (South American origin FBS; catalogue no.10270106), Penicillin-Streptomycin (catalogue no. 15140122), Phosphate buffered saline (PBS; catalogue no: 10010023) and Trypsin-EDTA (catalogue no. 25200056) were procured from GIBCO, Invitrogen, USA. ACK lysing buffer (catalogue no. A1049201) was purchased from Thermo Fisher Scientific, USA.
Hydrogen tetrachloroaurate 3
Manufactured by Merck Group
Sourced in United Kingdom
Hydrogen tetrachloroaurate (III) is a chemical compound with the formula HAuCl4. It is a yellow, crystalline solid that is soluble in water. The compound is used as a precursor in the synthesis of various gold compounds and nanoparticles for various applications in research and industry.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Merck Group (3 mentions)
Silver nitrate, by Merck Group (2 mentions)
Phosphate buffered saline (pbs), by Merck Group (2 mentions)
Hydrogen peroxide, by Merck Group (2 mentions)
Sodium borohydride, by Merck Group (2 mentions)
Ack lysing buffer, by Thermo Fisher Scientific (2 mentions)
Sodium citrate tribasic dehydrate, by Merck Group (1 mentions)
Hyaluronic acid, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Polyethylene glycol, by Merck Group (1 mentions)
N hydroxysuccinimide nhs, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride edc, by Merck Group (1 mentions)
Pure water, by Merck Group (1 mentions)
Potassium phosphate monobasic and dibasic, by Merck Group (1 mentions)
Hydrofluoric acid, by Merck Group (1 mentions)
α synuclein, by Merck Group (1 mentions)
10 protocols using hydrogen tetrachloroaurate 3
1
Synthesis of Multifunctional Gold Nanoparticles
2
Synthesizing Gold Nanoparticles
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Hydrogen tetrachloroaurate
(III) (HAuCl4·3H2O) and potassium phosphate
monobasic and
dibasic were purchased from Sigma-Aldrich. All of the chemicals were
used as received. Finally, pure water (18.2 MU cm; Millipore) was
used for all experiments.
(III) (HAuCl4·3H2O) and potassium phosphate
monobasic and
dibasic were purchased from Sigma-Aldrich. All of the chemicals were
used as received. Finally, pure water (18.2 MU cm; Millipore) was
used for all experiments.
3
Characterization of TiO2 and ZnO NPs
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The TiO2 and ZnO nanoparticles were obtained from Nanostructured and Amorphous Materials Inc., Los Alamos, NM, USA.
Phosphate buffered saline (PBS), bovine serum albumin (BSA), water, hydrofluoric acid, silver nitrate, hydrogen tetrachloroaurate (III), ethanol, 2-propanol, β-amyloid fragment 1-40 (βA), and α-synuclein were obtained from Sigma Aldrich. All reagents and solvents used in this study were analytical grades.
Phosphate buffered saline (PBS), bovine serum albumin (BSA), water, hydrofluoric acid, silver nitrate, hydrogen tetrachloroaurate (III), ethanol, 2-propanol, β-amyloid fragment 1-40 (βA), and α-synuclein were obtained from Sigma Aldrich. All reagents and solvents used in this study were analytical grades.
4
Antioxidant Activity and Polyphenol Content Assay
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2,2-Di(4-tert-octylphenyl)-1-picrylhydrazyl
(DPPH), the Tris base
(C4H11NO3), hydrochloric acid (HCl),l -ascorbic acid (C6H8O6), phosphate-buffered
saline (PBS), trichloroacetic acid (C2HCl3O2), ferric chloride (FeCl3), Folin and Ciocalteu’s
phenol reagent, sodium carbonate (Na2CO3), gallic
acid (C7H6O5), sodium nitrite (NaNO2), aluminum chloride (AlCl3), sodium hydroxide
(NaOH), catechin (C15H14O6), hydrogen tetrachloroaurate
(III) (HAuCl4·3H2O), hydrogen peroxide
(H2O2, 30%), the HRP enzyme, 1,4-dithiothreitol
(DTT), sodium acetate (NaOAc), and ethylenediaminetetraacetic acid
(EDTA) were all purchased from Sigma-Aldrich (UK). 3,3′,5,5′-Tetramethylbenzidine
(TMB) was purchased from Thermo Fisher Scientific (UK). PD-10 desalting
columns were purchased from GE Healthcare Life Sciences. Solid cheese
(cheddar) was purchased from a local supermarket (Tesco, Belfast,
UK).
(DPPH), the Tris base
(C4H11NO3), hydrochloric acid (HCl),
saline (PBS), trichloroacetic acid (C2HCl3O2), ferric chloride (FeCl3), Folin and Ciocalteu’s
phenol reagent, sodium carbonate (Na2CO3), gallic
acid (C7H6O5), sodium nitrite (NaNO2), aluminum chloride (AlCl3), sodium hydroxide
(NaOH), catechin (C15H14O6), hydrogen tetrachloroaurate
(III) (HAuCl4·3H2O), hydrogen peroxide
(H2O2, 30%), the HRP enzyme, 1,4-dithiothreitol
(DTT), sodium acetate (NaOAc), and ethylenediaminetetraacetic acid
(EDTA) were all purchased from Sigma-Aldrich (UK). 3,3′,5,5′-Tetramethylbenzidine
(TMB) was purchased from Thermo Fisher Scientific (UK). PD-10 desalting
columns were purchased from GE Healthcare Life Sciences. Solid cheese
(cheddar) was purchased from a local supermarket (Tesco, Belfast,
UK).
5
Synthesis and Characterization of Gold Nanoparticles
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Hydrogen
tetrachloroaurate(III) (HAuCl4) (40% solution in dilute
hydrochloric acid), sodium hydroxide
(NaOH), phenol red, sodium borohydride (NaBH4), ascorbic
acid (AA), silver nitrate, potassium iodide (KI), potassium platinum(II)
chloride, cetyltrimethylammonium bromide (CTAB), and cetyltrimethylammonium
chloride (CTAC) were purchased from Sigma-Aldrich; hydrogen peroxide
solution (30%) and cerium acetate (Ce(AC3)) were purchased
from Roth; poly(vinyl alcohol) (PVA) (Mw = 125,000 g·mol–1, 88% hydrolyzed) was purchased
from Polysciences Inc., and glutaraldehyde (GA, 50% aqueous solution)
was purchased from Merck. All experiments performed in an aqueous
environment were conducted using Milli-Q water (pH = 6.90, 18.2 MΩ).
tetrachloroaurate(III) (HAuCl4) (40% solution in dilute
hydrochloric acid), sodium hydroxide
(NaOH), phenol red, sodium borohydride (NaBH4), ascorbic
acid (AA), silver nitrate, potassium iodide (KI), potassium platinum(II)
chloride, cetyltrimethylammonium bromide (CTAB), and cetyltrimethylammonium
chloride (CTAC) were purchased from Sigma-Aldrich; hydrogen peroxide
solution (30%) and cerium acetate (Ce(AC3)) were purchased
from Roth; poly(vinyl alcohol) (PVA) (Mw = 125,000 g·mol–1, 88% hydrolyzed) was purchased
from Polysciences Inc., and glutaraldehyde (GA, 50% aqueous solution)
was purchased from Merck. All experiments performed in an aqueous
environment were conducted using Milli-Q water (pH = 6.90, 18.2 MΩ).
6
Fucoidan-mediated Synthesis of Gold Nanoparticles
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The study was performed using P. aeruginosa PAO1 KCTC 1637 obtained from Korean Collection for Type Cultures, Daejeon, Korea as the reference strain. The liquid and solid media used for the growth and cultivation of P. aeruginosa were tryptic soya broth (TSB; Difco Laboratory Inc., Detroit, MI, USA) and tryptic soya agar (TSA) plate. The pH of the media was adjusted to 7.2. Fucoidan (≥95%) sourced from Fucus vesiculosus) and hydrogen tetrachloroaurate (III) were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). All the reagents and chemicals used in the present study were of analytical grade. The growth condition of P. aeruginosa was aerobic and the growth temperature was maintained at 35 °C throughout the experiment.
7
Spectroscopic Characterization of Gold Nanoparticles
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Hydrogen
tetrachloroaurate(III) (≥99.9%), HSA, BSA, ovalbumin, lipase
and guanidine hydrochloride were purchased from Sigma. GSH, hemoglobin,
horseradish peroxidase, lysozyme, RNase A, carbonic anhydrase, immunoglobulin
G, trypsin, and BCA kit were purchased from Sangon Biotech. Phosphate
buffers (20 mM) with pH 4.0, 5.0, 6.0, 7.0, 8.0, and 9.0 were prepared
by mixing solutions of Na2HPO4 (20 mM) and NaH2PO4 (20 mM). The buffer pH was tuned by HCl/NaOH
solution.
Luminescence study was performed on a Shimadzu RF-5301PC
fluorometer. Time-resolved luminescence spectra were measured on an
Edinburgh FS 920 fluorometer. UV–vis spectra measurement was
performed on a Shimadzu UV-1800 spectrophotometer. TEM micrographs
were obtained by a FEI Tecnai G2-Twin microscope. XPS were obtained
using an ESCALAB-MKII spectrometer. CD spectra were carried out on
a Bio-Logic MOS 500 circular dichroism spectrometer. Secondary structural
contents were calculated by using the Dicro 2000 program. DLS and
ζ-potential measurement were performed on a Brookhaven ZetaPlus
apparatus.
tetrachloroaurate(III) (≥99.9%), HSA, BSA, ovalbumin, lipase
and guanidine hydrochloride were purchased from Sigma. GSH, hemoglobin,
horseradish peroxidase, lysozyme, RNase A, carbonic anhydrase, immunoglobulin
G, trypsin, and BCA kit were purchased from Sangon Biotech. Phosphate
buffers (20 mM) with pH 4.0, 5.0, 6.0, 7.0, 8.0, and 9.0 were prepared
by mixing solutions of Na2HPO4 (20 mM) and NaH2PO4 (20 mM). The buffer pH was tuned by HCl/NaOH
solution.
Luminescence study was performed on a Shimadzu RF-5301PC
fluorometer. Time-resolved luminescence spectra were measured on an
Edinburgh FS 920 fluorometer. UV–vis spectra measurement was
performed on a Shimadzu UV-1800 spectrophotometer. TEM micrographs
were obtained by a FEI Tecnai G2-Twin microscope. XPS were obtained
using an ESCALAB-MKII spectrometer. CD spectra were carried out on
a Bio-Logic MOS 500 circular dichroism spectrometer. Secondary structural
contents were calculated by using the Dicro 2000 program. DLS and
ζ-potential measurement were performed on a Brookhaven ZetaPlus
apparatus.
8
Mycobiosynthesis of Metal Nanoparticles
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A. kambarensis PFCC 411‐132 was used for the mycobiosynthesis of MNPs. The fungus was obtained from Pathogenic Fungi Culture Collection of the Pasteur institute of Iran. All culture media and chemicals including potato dextrose broth (PDB; Merck), Sabouraud dextrose agar (SDA; Merck), copper (II) sulphate pentahydrate salt (CuSO4·5H2O, 99% purity, Sigma‐Aldrich), silver nitrate (AgNO3, 99% purity, Sigma‐Aldrich), hydrogen tetrachloroaurate (III) (HAuCl4·3H2O, 99% purity, Sigma‐Aldrich), and zinc sulphate (ZnSO4·7H2O, 99% purity, Sigma‐Aldrich) were of analytical grade purchased from international companies.
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A. kambarensis PFCC 411‐132 was used for the mycobiosynthesis of MNPs. The fungus was obtained from Pathogenic Fungi Culture Collection of the Pasteur institute of Iran. All culture media and chemicals including potato dextrose broth (PDB; Merck), Sabouraud dextrose agar (SDA; Merck), copper (II) sulphate pentahydrate salt (CuSO4·5H2O, 99% purity, Sigma‐Aldrich), silver nitrate (AgNO3, 99% purity, Sigma‐Aldrich), hydrogen tetrachloroaurate (III) (HAuCl4·3H2O, 99% purity, Sigma‐Aldrich), and zinc sulphate (ZnSO4·7H2O, 99% purity, Sigma‐Aldrich) were of analytical grade purchased from international companies.
9
Peptide Synthesis and Enzyme Assays
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The peptide Gly-Pro-Asp-Cys (~ 90%) pure was purchased from Thermo Fisher Scientific Co. Ltd (Germany), Val-Pro-NH-(CH 2 ) 2 -NH-Asp-Cys (> 95%) pure was purchased from Cambridge Research Biochemicals (UK). DPP-IV enzyme from porcine kidney was purchased from Merck Chemicals (Germany). Human lysozyme, trypsin from porcine pancreas, hydrogen tetrachloroaurate(III) (HAuCl 4 .3H 2 O), 99.99% pure, and sodium citrate dihydrate (Na 3 C 6 H 5 O 7 .2H 2 O), 99% pure, were purchased from Sigma-Aldrich Co. Ltd (UK) and used without further purification. Thrombin from bovine plasma was purchased from GE HealthCare (UK). Normal human serum control was purchased from Thermo Scientific.
The DPP-IV solutions were prepared in 50mM Tris buffer solution of pH 8.3. A 10 mМ citrate buffer solution at pH 6 was used to dissolve the GPDC peptide, VP-EN-DC was dissolved in 50 mМ Glycine buffer solution (pH 10).All solutions were prepared using deionized water with a resistivity of 18.2 MΩ cm -1 prepared with a Milli-Q Academic Purification equipment from Millipore (UK).
The DPP-IV solutions were prepared in 50mM Tris buffer solution of pH 8.3. A 10 mМ citrate buffer solution at pH 6 was used to dissolve the GPDC peptide, VP-EN-DC was dissolved in 50 mМ Glycine buffer solution (pH 10).All solutions were prepared using deionized water with a resistivity of 18.2 MΩ cm -1 prepared with a Milli-Q Academic Purification equipment from Millipore (UK).
10
Synthesis and Characterization of Organic Compounds
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Sodium hydrogen carbonate, sodium sulfate (Na2SO4) and 6N hydrochloric acid (HCl) were purchased from Scharlau; sodium hydroxide (NaOH) from Merck, 4-methylpyridine, 1,3bis(bromomethyl)benzene, 1-decanol, 1-octadecanol, 4-chloropyridine hydrochloride, hydrogen peroxide (H2O2), sodium borohydride (NaBH4), hydrogen tetrachloroaurate (III) (HAuCl4), sodium citrate tribasic dihydrate, potassium bromide (KBr), ibuprofen, piroxicam, tetraoctylammonium bromide, phosphate buffered saline (PBS) pH=7.4 and 5.2 were purchased from Sigma-Aldrich. Metallic sodium and ammonia 20 % were purchased from Panreac and hexa(ethylene glycol)alkyl thiol (HS-C11-(EG)6-OH) was purchased from Prochimia. Acetonitrile (MeCN), dichloromethane (CH2Cl2), methanol (MeOH), diethyl ether (Et2O), chloroform (CHCl3), ethanol (EtOH), dimethyl sulfoxide (DMSO), tetrahydrofuran (THF), dimethylformamide (DMF), toluene, dimethylsulfoxide-d6 (CD3)2SO and chloroform-d (CDCl3) were purchased from Sigma-Aldrich.
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