Mice were treated with 0.02 CC of 5 mg/mL EdU for 1 hour prior to sacrifice. EdU staining was performed following incubation of slides with secondary antibody. AF-647 azide, copper sulfate and EdU additive buffer and reaction buffers (Life Technology) were added to slides. Slides were incubated for 20 min at room temperature, washed and mounted for imaging.
Edu additive buffer and reaction buffers
Manufactured by Thermo Fisher Scientific
The EdU additive buffer and reaction buffers are specialized lab equipment designed for use in cell proliferation assays. The EdU additive buffer is used to incorporate the EdU (5-ethynyl-2'-deoxyuridine) molecule into newly synthesized DNA, while the reaction buffers are used to facilitate the subsequent detection of the incorporated EdU. These products provide the necessary components for performing cell proliferation studies, enabling researchers to accurately measure and analyze cell division and growth patterns.
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Lab products found in correlation
2 protocols using edu additive buffer and reaction buffers
1
In Vivo Nucleotide Incorporation Assay
2
In Vivo Nucleotide Incorporation Assay
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Mice were treated with 0.02 CC of 5 mg/mL EdU for 1 hour prior to sacrifice. EdU staining was performed following incubation of slides with secondary antibody. AF-647 azide, copper sulfate and EdU additive buffer and reaction buffers (Life Technology) were added to slides. Slides were incubated for 20 min at room temperature, washed and mounted for imaging.
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