iRGD and FFVLK-(PEG8)3 (MW = 1923.2 Da) were synthesized by Shanghai Dechi Biosciences Co., Ltd. (Shanghai, China). Ce6 was purchased from Dalian Meilun Biotech Co., Ltd. (Dalian, China). Bilirubin was gained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). d-SN38 was supplied by Prof. Jun Cao (Sichuan University, China). 4′,6-Diamidino-2-phenylindole (DAPI) and Lysotracker green was purchased from Life Technologies (Grand Island, USA). FITC Annexin V Apoptosis Detection Kit I was obtained from BD Pharmingen™ (Franklin Lakes, NJ, USA). Calcein-AM/PI Double Stain Kit was supplied by Yeasen (Shanghai, China). 24-well hanging cell culture inserts (8.0 μm, PET) were obtained from Corning Inc. (Kennebunk, ME, USA). All other reagents and solvents were purchased from commercial resources and used without further purification unless otherwise noted. Mouse mammary breast tumor cell line 4T1 was obtained from Chinese Academy of Sciences Cells Bank (Shanghai, China). 4T1 cells were incubated in RPMI-1640 cell culture medium supplemented with 10% fetal bovine serum (FBS) and 100 U/mL streptomycin, and 100 U/mL penicillin at 37 °C in a humidified 5% CO2 atmosphere.
Bilirubin
Manufactured by Tokyo Chemical Industry
Sourced in Japan
Bilirubin is a chemical compound used in laboratory settings. It is a reddish-yellow pigment that is produced by the breakdown of heme in the body. Bilirubin is commonly used as a standard reference material for various analytical techniques, such as spectrophotometry, to measure and quantify bilirubin levels in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Bilirubin, by Merck Group (2 mentions)
Pamidronate, by Tokyo Chemical Industry (2 mentions)
Calcein am pi double stain kit, by Yeasen (1 mentions)
Lysotracker green, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Fitc annexin 5 apoptosis detection kit 1, by BD (1 mentions)
Hepes, by Dojindo Laboratories (1 mentions)
Ethylene glycol tetraacetic acid (egta), by Dojindo Laboratories (1 mentions)
Lactate dehydrogenase assay kit, by Nanjing Jiancheng (1 mentions)
Anti cd31 antibody, by Wuhan Servicebio Technology (1 mentions)
Anti hif 1α antibody, by Abcam (1 mentions)
Dcfh da, by Meilun (1 mentions)
N n diisopropylcarbodiimide, by Tokyo Chemical Industry (1 mentions)
Anti ldhb, by Proteintech (1 mentions)
Chlorin e6, by J&K Scientific (1 mentions)
Anti c myc antibody, by Proteintech (1 mentions)
Cou 6, by Merck Group (1 mentions)
Anti ldha, by Wuhan Servicebio Technology (1 mentions)
Lactate detection kit, by Nanjing Jiancheng (1 mentions)
Acetaminophen, by Fujifilm (1 mentions)
7 protocols using bilirubin
1
Synthesis and Characterization of Dual-Targeting Nanomedicine
2
Bilirubin and Pamidronate Effects on SHED Dentinogenesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SHED (10 × 104 per 60 mm culture dish) were cultured under dentinogenic conditions with 0 μM bilirubin, 50 μM bilirubin (Merck), and 50 μM bilirubin plus 10 μM pamidronate (Tokyo Chemical Industry) according to previous studies [13 (link)]. Two and 4 weeks after the dentinogenic induction, the cultures were harvested for the examination of gene and protein expression and calcium deposition, respectively.
3
Pharmacological Reagent Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pharmacological reagents were obtained from Sigma-Aldrich, except for bilirubin, TEA (Tokyo Chemical Industry, Tokyo Japan), EGTA, and HEPES (Dojindo Molecular Technologies, Kumamoto, Japan). T16Ainh-A01 (2-[(5-ethyl-1,6-dihydro-4-methyl-6-oxo-2-pyrimidinyl)thio]-N-[4-(4-methoxyphenyl)-2-thiazolyl]-acetamide) and bilirubin were dissolved in dimethyl sulfoxide (DMSO) and 0.1 M NaOH, respectively, at a concentration of 30 mM as a stock solution.
4
Effect of Bilirubin and Pamidronate on SHED Bone Formation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SHED were pretreated with 0 μM bilirubin, 50 μM bilirubin (Merck), and 50 μM bilirubin plus 10 μM pamidronate (Tokyo Chemical Industry) for 7 days. The cells (4 × 106) were mixed with hydroxyapatite tricalcium phosphate (40 mg, Zimmer Inc., Warsaw, IN) and were subcutaneously transplanted into 8- to 10-week-old female Balb/c nude/nude (Japan CLEA, Shizuoka, Japan) as described previously [2 (link)]. The implants were harvested 4 weeks after the transplantation. Frozen sections were treated for hematoxylin and eosin staining or immunofluorescent microscopy. The mineralized tissue area is represented as a percentage of the total area as described previously [2 (link)].
5
Chlorin e6-Mediated Photodynamic Therapy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chlorin e6 was purchased from J&K Scientific Ltd. (Beijing, China). Diclofenac, morpholin-4-yl-acetic acid hydrochloride, 1-(3-dimethylaminopropyl)-3 ethylcarbodiimide hydrochloride (EDC), 4-dimethylaminopyridine (DMAP) and trimethylamine (TEA) were purchased from Aladdin (Shanghai, China). Bilirubin and N,N′-diisopropylcarbodiimide (DIC) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). mPEG2k-NH2 and Fmoc-NH-PEG2k-NH2 were purchased from Ponsure Biotechnology (Shanghai, China). Cou-6 was obtained from Sigma–Aldrich (USA). DiD and DCFH-DA were obtained from Meilunbio (Dalian, China). The annexin V-fluoresceine isothiocyanate (FITC) apoptosis detection kit and calcium-AM were obtained from Yeason (Shanghai, China). Lactate detection kit and lactate dehydrogenase assay kit were obtained from Nanjing Jiancheng Bioengineering Institute (Naijing, China). Anti-HIF-1α antibody, Cy3 goat anti-rabbit IgG (H + L) and Alexa fluor 647 goat anti-rabbit IgG (H + L) secondary antibody were purchased from Abcam (Hongkong, China). Anti-LDHA and anti-CD31 antibody were purchased from Servicebio Company (Wuhan, China). Anti-LDHB and anti-c-MYC antibody were purchased from Proteintech (Wuhan, China). Anti-VEGFα and anti-angiopoietin-2 antibody were purchased from Abclonal Company (Wuhan, China).
6
Bilirubin Preparation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bilirubin was purchased from Tokyo chemical industry Co., Ltd. (Tokyo, Japan), solubilized in 0.2 N sodium hydroxide and adjusted with 0.5 M hydrochloride acid to a pH of 7.4–7.6, as in a previous study [34 (link)]. The final concentration of Bilirubin was diluted in saline solution; and stored –20 °C until use, preventing exposure to the light. All manipulations were carried out on a clean bench.
7
Cytotoxicity Evaluation of Drugs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cells were treated with SN-38 (10, 50, and 100 μM), acetaminophen (5, 10, and 20 mM; FUJIFILM Wako Pure Chemical), and bilirubin (1, 5, 10, 25, and 50 μM; Tokyo Chemical Industry, Tokyo, USA) for 2 days. The cell viability was measured using the CellTiter-Glo 3D Cell Viability Assay (Promega, WI, USA). The cell viability was calculated as a percentage of that in the cells treated with vehicle (DMSO) only.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!