Peg thiol

Manufactured by Creative PEGWorks

PEG-thiol is a polyethylene glycol (PEG) derivative containing a thiol functional group. It is a versatile reagent used in a variety of chemical and biological applications.

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Lab products found in correlation

Glass vial, by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Avantor (1 mentions) Polystyrene beads, by Merck Group (1 mentions) Micro cover glass, by Avantor (1 mentions) Dulbecco s phosphate buffered saline, by Avantor (1 mentions) Biotin peg thiol, by Nanocs (1 mentions) Milli q gradient water purification system, by Merck Group (1 mentions) Tris 2 carboxyethyl phosphine hydrochloride, by Thermo Fisher Scientific (1 mentions) Neutravidin, by Thermo Fisher Scientific (1 mentions) Hydrogen peroxide, by Merck Group (1 mentions) Sulfuric acid, by Merck Group (1 mentions) Ethyl alcohol, by Merck Group (1 mentions)

2 protocols using peg thiol

Filomicelle Depot Fabrication Protocol

Cited 1 time

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Filomicelles were self-assembled from PEG₄₅-b-PPS₄₄ and VS- PEG₄₅-b-PPS₄₄ polymers via thin-film rehydration method in PBS as described previously (Karabin et al., 2018 (link)). Briefly, the mixture of PEG₄₅-b-PPS₄₄ (40 mg) and VS- PEG₄₅-b-PPS₄₄ (10 mg) polymers were dissolved in 1 ml dichloromethane within 1.8 ml glass vials (ThermoFisher Scientific) and placed under vacuum to remove the solvent. The resulting thin films were hydrated with 493 μl of phosphate-buffered saline (PBS) and gently mixed using a Stuart SB3 rotator for at least 36 h. To prepare FM-depots, eight-arm PEG-thiol (10% w/v in PBS solution, Creative PEGWorks) was added to FM solution corresponding to a 1.1:1 molar ratio of thiol:vinyl sulfone. The obtained mixture was vortexed and a Teflon mold (6 mm) was filled with 55 μl of the mixture. The FM-depots were formed after incubation at 37°C in a humidified environment for 30 min.

Yi S., Karabin N.B., Zhu J., Bobbala S., Lyu H., Li S., Liu Y., Frey M., Vincent M, & Scott E.A. (2020). An Injectable Hydrogel Platform for Sustained Delivery of Anti-inflammatory Nanocarriers and Induction of Regulatory T Cells in Atherosclerosis. Frontiers in Bioengineering and Biotechnology, 8, 542.

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Biotinylated Aptamer Surface Preparation

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Biotin PEG thiol (MW 1000 Da) was purchased from Nanocs. PEG thiol (MW 550 Da) was purchased from Creative PEGWorks. Neutravidin (NA) and tris(2-carboxyethyl)phosphine hydrochloride (TCEP) were purchased from Thermo Fisher Scientific. Polystyrene beads (0.6 µm mean particle size), sulfuric acid (95.0–98.0%), hydrogen peroxide (30% w/w in H₂O), and ethyl alcohol (200 proof) were purchased from Sigma-Aldrich. Paraformaldehyde (16% w/v aq soln, methanol free), Dulbecco’s Phosphate Buffered Saline (1×, without calcium and magnesium), and micro cover glass (No. 1, 18 mm × 18 mm) were purchased from VWR. Lysogeny broth and granulated agar were purchased from BD Difco. Biotinylated aptamer (Bt-aptamer, 5′[Bt]-ATACCA-GCTTATTCAATTCCCCCGTTGCTTTCGCTTTTCCTT-TCGCTTTTGTTCGTTTCGTCCCTGCTTCCTTTCTTG-AGATAGTAAGTGCAATCT3′) and fluorescently labeled biotinylated aptamer (6FAM-aptamer-Bt, 5′[6-carboxyfluorescein-ATACCAGCTTATTCAATTCCCCCGTTGCTT-TCGCTTTTCCTTTCGCTTTTGTTCGTTTCGTCCCTG-CTTCCTTTCTTGAGATAGTAAGTGCAATCT-[Bt]3′) were synthesized by Sigma-Aldrich. Deionized (DI) water used in all experiments was prepared using a Milli-Q Gradient water purification system with a resistivity of 18.2 MΩ·cm at 25 °C (Millipore).

Hu J., Fu K, & Bohn P.W. (2018). Whole-Cell Pseudomonas aeruginosa Localized Surface Plasmon Resonance Aptasensor. Analytical chemistry, 90(3), 2326-2332.

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