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Siv gp120

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SIV gp120 is a recombinant protein that corresponds to the surface glycoprotein of the Simian Immunodeficiency Virus. It is a laboratory tool used for research purposes.

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Lab products found in correlation

Magnetic carboxylated beads, by Bio-Rad (3 mentions) Tetanus toxoid, by Merck Group (3 mentions) Influenza hemagglutinin h1cal2009, by Sino Biological (3 mentions) Spike 2, by ACROBiosystems (2 mentions) Sars cov 2 spike 1, by Sino Biological (2 mentions) Sars cov 2 and hku 1 spike trimers, by BPS Biosciences (2 mentions) Sars cov and nl63 spike trimers, by BPS Biosciences (2 mentions) Nucleoprotein, by ACROBiosystems (2 mentions) Nun c5227, by ACROBiosystems (1 mentions) S2n c52h5, by ACROBiosystems (1 mentions) Sars cov 2 spike trimers, by BPS Biosciences (1 mentions)

Close spelling variants of this product

Gp120

4 protocols using siv gp120

1

Multiplex SARS-CoV-2 Antibody Detection

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As previously described25 , a custom multiplex bead array was designed and coupled with SARS-CoV-2 spike 1 (Sino Biological), spike 2 (ACRO Biosystems), RBD (BEI Resources) and nucleoprotein (ACRO Biosystems), as well as SARS and hCoV (229E, NL63, HKU1, OC43) spikes and nucleoproteins (Sino Biological) (Supplementary Fig. 5). In addition, SARS-CoV-2 and HKU-1 spike trimers (kind gifts from Adam K. Wheatley), as well as SARS-CoV and NL63 spike trimers (BPS Bioscience) were also coupled. Tetanus toxoid (Sigma-Aldrich), influenza hemagglutinin (H1Cal2009; Sino Biological) and SIV gp120 (Sino Biological) were also included in the assay as positive and negative controls respectively. Antigens were covalently coupled to magnetic carboxylated beads (Bio Rad) using a two-step carbodiimide reaction and blocked with 0.1% BSA, before being resuspended and stored in PBS 0.05% sodium azide.
Zhang W., Chua B., Selva K., Kedzierski L., Ashhurst T., Haycroft E., Shoffner S., Hensen L., Boyd D., James F., Mouhtouris E., Kwong J., Chua K., Drewett G., Copaescu A., Dobson J., Rowntree L., Habel J., Allen L., Koay H.F., Neil J., Gartner M., Lee C., Andersson P., Seemann T., Sherry N., Amanat F., Krammer F., Londrigan S., Wakim L., King N., Godfrey D., Mackay L., Thomas P., Nicholson S., Arnold K., Chung A., Holmes N., Smibert O., Trubiano J., Gordon C., Nguyen T, & Kedzierska K. (2021). Immune responses in COVID-19 respiratory tract and blood reveal mechanisms of disease severity. Research Square.
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2

Characterization of SARS-CoV-2 Antigens

Cited 1 time
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SARS-CoV-2 antigens of the ancestral wild-type virus (B.1) are as follows: spike S1 (Sino Biological; 40,591-V08H), spike S2 (ACRO Biosystems; S2N-C52H5), nucleoprotein (ACRO Biosystems; NUN-C5227) and spike trimer (provided by Adam Wheatley) [32 (link)]. Influenza H1Cal2009 (Sino Biological; 11,085-V08H) and SIVgp120 (Sino Biological; 40,415-V08H) were used as positive and negative controls, respectively (See Supplementary Table 1).
Haycroft E.R., Davis S.K., Ramanathan P., Lopez E., Purcell R.A., Tan L.L., Pymm P., Wines B.D., Hogarth P.M., Wheatley A.K., Juno J.A., Redmond S.J., Gherardin N.A., Godfrey D.I., Tham W.H., Selva K.J., Kent S.J, & Chung A.W. (2023). Antibody Fc-binding profiles and ACE2 affinity to SARS-CoV-2 RBD variants. Medical Microbiology and Immunology, 212(4), 291-305.
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3

SARS-CoV-2 Multiplex Serological Assay

Cited 1 time
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As previously described25 (link), a custom multiplex bead array was designed and coupled with SARS-CoV-2 spike 1 (Sino Biological), spike 2 (ACRO Biosystems), RBD (BEI Resources) and nucleoprotein (ACRO Biosystems), as well as SARS and hCoV (229E, NL63, HKU1, OC43) spikes and nucleoproteins (Sino Biological) (Supplementary Table 5). In addition, SARS-CoV-2 and HKU-1 spike trimers (kind gifts from Adam K. Wheatley), as well as SARS-CoV and NL63 spike trimers (BPS Bioscience) were also coupled. Tetanus toxoid (Sigma-Aldrich), influenza hemagglutinin (H1Cal2009; Sino Biological) and SIV gp120 (Sino Biological) were included in the assay as positive and negative controls respectively. Antigens were covalently coupled to magnetic carboxylated beads (Bio Rad) using a two-step carbodiimide reaction and blocked with 0.1% BSA, before being resuspended and stored in PBS 0.05% sodium azide.
Zhang W., Chua B.Y., Selva K.J., Kedzierski L., Ashhurst T.M., Haycroft E.R., Shoffner-Beck S.K., Hensen L., Boyd D.F., James F., Mouhtouris E., Kwong J.C., Chua K.Y., Drewett G., Copaescu A., Dobson J.E., Rowntree L.C., Habel J.R., Allen L.F., Koay H.F., Neil J.A., Gartner M.J., Lee C.Y., Andersson P., Khan S.F., Blakeway L., Wisniewski J., McMahon J.H., Vine E.E., Cunningham A.L., Audsley J., Thevarajan I., Seemann T., Sherry N.L., Amanat F., Krammer F., Londrigan S.L., Wakim L.M., King N.J., Godfrey D.I., Mackay L.K., Thomas P.G., Nicholson S., Arnold K.B., Chung A.W., Holmes N.E., Smibert O.C., Trubiano J.A., Gordon C.L., Nguyen T.H, & Kedzierska K. (2022). SARS-CoV-2 infection results in immune responses in the respiratory tract and peripheral blood that suggest mechanisms of disease severity. Nature Communications, 13, 2774.
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4

Multiplex Bead Array for COVID-19 Serology

Cited 1 time
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A custom multiplex bead array was designed (Supplemental Table 3) and coupled with SARS-CoV-1 and SARS-CoV-2 spike-1 (stem, Sino Biological), spike-2 (head, ACRO Biosystems), RBD (BEI Resources), and N (ACRO Biosystems), as described previously (45 (link)). In addition, SARS-CoV-2 spike trimers (provided in-house) and SARS-CoV-2 spike trimers (BPS Bioscience) were included. Tetanus toxoid (Sigma-Aldrich), influenza hemagglutinin (H1Cal2009, Sino Biological), and SIV-gp120 (Sino Biological) were included as positive and negative control antigens, respectively. Antigens were covalently coupled to magnetic carboxylated beads (Bio-Rad) using a 2-step carbodiimide reaction and blocked with 0.1% BSA, before being resuspended and stored in PBS with 0.05% sodium azide until use.
Habel J.R., Chua B.Y., Kedzierski L., Selva K.J., Damelang T., Haycroft E.R., Nguyen T.H., Koay H.F., Nicholson S., McQuilten H.A., Jia X., Allen L.F., Hensen L., Zhang W., van de Sandt C.E., Neil J.A., Pragastis K., Lau J.S., Jumarang J., Allen E.K., Amanant F., Krammer F., Wragg K.M., Juno J.A., Wheatley A.K., Tan H.X., Pell G., Walker S., Audsley J., Reynaldi A., Thevarajan I., Denholm J.T., Subbarao K., Davenport M.P., Hogarth P.M., Godfrey D.I., Cheng A.C., Tong S.Y., Bond K., Williamson D.A., McMahon J.H., Thomas P.G., Pannaraj P.S., James F., Holmes N.E., Smibert O.C., Trubiano J.A., Gordon C.L., Chung A.W., Whitehead C.L., Kent S.J., Lappas M., Rowntree L.C, & Kedzierska K. (2023). Immune profiling of SARS-CoV-2 infection during pregnancy reveals NK cell and γδ T cell perturbations. JCI Insight, 8(7), e167157.
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