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Tc20 automated cell counter

Manufactured by Abcam
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The TC20 automated cell counter is a compact and user-friendly instrument designed for accurate cell counting and cell viability analysis. It utilizes advanced optics and image processing technology to provide reliable and consistent results.

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Lab products found in correlation

Deproteinizing sample preparation kit, by Abcam (2 mentions) Fluorimetric assay kit, by Abcam (2 mentions) Mammalian lysis buffer, by Abcam (2 mentions) Spectramax id5, by Molecular Devices (2 mentions)

2 protocols using tc20 automated cell counter

1

Fluorimetric Assay for Cellular Glutathione

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Cellular Glutathione was measured using a Fluorimetric Assay Kit (Abcam, Cat. ab205811) and following the manufacturer’s protocol. Briefly, 100–300 thousand cells (counted using a Bio-Rad, TC20 automated cell counter), were resuspended in 100 µL of ice cold 1X mammalian lysis buffer (Abcam, Cat. ab179835) and deproteinized with the deproteinizing sample preparation kit (BioVision, Cat. K808–200). The sample was then incubated with the reaction mix for 15–20 min, and the levels of reduced GSH were measured using a fluorescence microplate reader (Molecular Devices, SpectraMax iD5) at Ex/Em = 490/520 nm. All experiments were performed in triplicate.
Chavdoula E., Anastas V., Ferlita A.L., Aldana J., Carota G., Spampinato M., Soysal B., Cosentini I., Parashar S., Sircar A., Nigita G., Sehgal L., Freitas M.A, & Tsichlis P.N. (2023). Transcriptional regulation of amino acid metabolism by KDM2B, in the context of ncPRC1.1 and in concert with MYC and ATF4. bioRxiv.
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2

Cellular Glutathione Quantification Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cellular Glutathione was measured using a Fluorimetric Assay Kit (Abcam, Cat. ab205811) and following the manufacturer's protocol. Briefly, 100-300 thousand cells (counted using a Bio-Rad, TC20 automated cell counter), were resuspended in 100 µL of ice cold 1X mammalian lysis buffer (Abcam, Cat. ab179835) and deproteinized with the deproteinizing sample preparation kit (BioVision, Cat. K808-200). The sample was then incubated with the reaction mix for 15-20 min, and the levels of reduced GSH were measured using a fluorescence microplate reader (Molecular Devices, SpectraMax iD5) at Ex/Em = 490/520 nm. All experiments were performed in triplicate.
Chavdoula E., Anastas V., La Ferlita A., Aldana J., Carota G., Spampinato M., Soysal B., Cosentini I., Parashar S., Sircar A., Nigita G., Sehgal L., Freitas M.A, & Tsichlis P.N. (2024). Transcriptional regulation of amino acid metabolism by KDM2B, in the context of ncPRC1.1 and in concert with MYC and ATF4. Metabolism: clinical and experimental, 150.
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