Cells were stained with combinations of the following mAbs: anti-CD4–PE/cyanine7 (Cy7) and anti-CD25–allophycocyanin (APC; BD). Cells were washed, fixed, permeabilized, and stained to detect intracellular cytokines with mAbs recognizing IL-17 and forkhead box P3 (FOXP3; eBioscience, San Diego, CA, USA). Cells were analyzed on a FACS Calibur flow cytometry system (BD).
Anti cd25 allophycocyanin apc
Manufactured by BD
Anti-CD25–allophycocyanin (APC) is a fluorescently labeled antibody that binds to the CD25 antigen. It is a tool used in flow cytometry applications to identify and quantify cells expressing the CD25 marker.
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2 protocols using anti cd25 allophycocyanin apc
1
Multiparametric Immune Cell Profiling
2
T Cell Subsets in Arthritis
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Peripheral blood mononuclear cells (PBMCs) were isolated at 8 weeks after induction of arthritis. To analyze the population of T helper cells, the isolated PBMCs were stained with anti-CD4 peridinin chlorophyll protein complex (PerCP; BD Biosciences, San Diego, CA, USA), anti-IFNγ-fluorescein isothiocyanate (FITC; BD Biosciences), and anti-IL-17A eFluor 660 (eBioscience, San Diego, CA, USA). For regulatory T cells, the PBMCs were stained with anti-CD4 PerCP (BD Biosciences), anti-CD25-allophycocyanin (APC; BD Biosciences), and anti-Foxp3-PE (BD Biosciences). Cells were permeabilized and fixed with CytoPerm/CytoFix (BD Biosciences) according to the manufacturer’s protocol. Flow cytometry was performed using a FACSCalibur cytometer (BD Biosciences).
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