Inhibitor control anti mir nc

Overexpressed UCA1 (pcDNA-UCA1) clones were based on the pcDNA-3.1 vector (Invitrogen). The UCA1 fragments were obtained by PCR and inserted into the XbaI/EcoRI sites. The constructs were identified and sequenced. The primers used were listed as follows: UCA1 forward primer F: 5′-CCGCTCGAGAGCGCGTGTGGCGGCCGAGCAC-3′, and UCA1 reverse primer R: 5′-CGCGGATCC AGACACGAGGCCGGCCACGCCACG-3′. Three specific siRNA targeting UCA1 (si-UCA1#1, si-UCA1#2, and si-UCA1#3) and scrambled siRNA control (si-NC) were obtained from GenePharma (Shanghai, China). The sequences were described as follows: si-UCA1#1: 5′-GGACAACAGUACACGCAUATT-3′; si-UCA1#2: 5′-GCCACCUACAUUAAAGCUATT-3′; si-UCA1#3: 5′-GACCAGACCCTACCCGGTCATTTATUATT-3′; miR-143 mimic (miR-143), scrambled mimic control (miR-NC), miR-143 inhibitor (anti-miR-143), and inhibitor control (anti-miR- NC) were purchased from RiboBio (Guangzhou, China). All these plasmids and oligonucleotides were transfected into cells by Lipofectamine 2000 reagent (Invitrogen) following the manufacturer’s instructions.