Megaview 3 digital ccd

Manufactured by Olympus

The Megaview III digital CCD is a high-performance camera designed for microscopy applications. It features a large-format CCD sensor that captures detailed images with high resolution and sensitivity. The camera is capable of fast image acquisition and provides a flexible digital interface for seamless integration with microscope systems.

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Lab products found in correlation

Tecnai g2 spirit, by Olympus (1 mentions) Tecnai g2 spirit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Megaview 3 CCD digital camera MegaView III CCD MegaView III

3 protocols using megaview 3 digital ccd

Exosome Visualization via Negative Staining

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Isolated exosomes were allowed to adsorb to freshly ionized 300 mesh formvar/carbon-coated grids, washed briefly through 5–7 puddles of ddH₂O; and negatively stained in 2% aqueous uranyl acetate. Images were acquired using a transmission electron microscope (FEI Tecnai G2 Spirit) with TWIN Lens operating at 100 kV with an Olympus Soft Imaging System Megaview III digital CCD.

Zhang S., Eitan E., Wu T.Y, & Mattson M.P. (2017). Intercellular transfer of pathogenic α-synuclein by extracellular vesicles is induced by the lipid peroxidation product 4-hydroxynonenal. Neurobiology of aging, 61, 52-65.

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Negative Staining of Extracellular Vesicles

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A drop of EV preparations (suspended in PBS) was added to a freshly ionized 300 mesh formvar/carbon coated grid and incubated for 5 minutes to allow adherence of the EVs to the grid. The grid was then washed through 5-7 puddles of ddH₂O; and negatively stained in 2% aqueous uranyl acetate for 30 seconds. Images were acquired using a FEI Tecnai G2 Spirit transmission electron microscope with TWIN Lens operating at 100 kV and an Olympus Soft Imaging System Megaview III digital CCD.

Eitan E., Hutchison E.R., Marosi K., Comotto J., Mustapic M., Nigam S.M., Suire C., Maharana C., Jicha G.A., Liu D., Machairaki V., Witwer K.W., Kapogiannis D, & Mattson M.P. (2016). Extracellular vesicle-associated Aβ mediates trans-neuronal bioenergetic and Ca2+-handling deficits in Alzheimer’s disease models. NPJ Aging and Mechanisms of Disease, 2, 16019-.

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Negative Staining and TEM Imaging of EVs

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EVs were allowed to adsorb to freshly ionized 300 mesh formvar/carbon coated grids then washed briefly through 5–7 puddles of ddH₂O and subsequently negatively stained in 2% aqueous uranyl acetate. Images were taken with transmission electron microscopy (FEI Tecnai G2 Spirit) with TWIN Lens operating at 100 kV and using an Olympus Soft Imaging System Megaview III digital CCD.

Eitan E., Green J., Bodogai M., Mode N.A., Bæk R., Jørgensen M.M., Freeman D.W., Witwer K.W., Zonderman A.B., Biragyn A., Mattson M.P., Noren Hooten N, & Evans M.K. (2017). Age-Related Changes in Plasma Extracellular Vesicle Characteristics and Internalization by Leukocytes. Scientific Reports, 7, 1342.

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