Log In Sign up
The largest database of trusted experimental protocols

Butaclamol hydrochloride

Manufactured by Merck Group
Visit Supplier
Sourced in Sao Tome and Principe

(+)-Butaclamol hydrochloride is a chemical compound that functions as a dopamine receptor antagonist. It is used as a research tool in the study of dopaminergic systems in the central nervous system.

Automatically generated - may contain errors

Lab products found in correlation

Quinpirole, by Merck Group (2 mentions) Hepes sodium salt, by Merck Group (2 mentions) 5 hydroxytryptamine creatine sulfate, by Merck Group (2 mentions) Isoproterenol bitartrate, by Merck Group (2 mentions) Hbss 10x, by Thermo Fisher Scientific (2 mentions) Dopamine hydrochloride, by Merck Group (2 mentions) Desipramine hydrochloride, by Merck Group (1 mentions) Hydroxyurea, by Merck Group (1 mentions) α methyl dl tyrosine, by Merck Group (1 mentions) Methylphenidate hydrochloride, by Merck Group (1 mentions) 3h raclopride, by PerkinElmer (1 mentions) Mecamylamine hydrochloride, by Merck Group (1 mentions) Picrotoxin, by Merck Group (1 mentions) Mk 801 hydrogen maleate, by Merck Group (1 mentions) Cgp 54626 hydrochloride, by Bio-Techne (1 mentions)

Close spelling variants of this product

(+)-butaclamol Hydrochlorides

5 protocols using butaclamol hydrochloride

1

Synthesis and Characterization of Neuroactive Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
All compounds and aripiprazole were synthesized as described under General Chemistry Procedures. Dopamine hydrochloride, (−)-quinpirole, (+)-butaclamol hydrochloride, 5-hydroxytryptamine creatine sulfate, (−)-isoproterenol bitartrate, and HEPES sodium salt were purchased from Sigma-Aldrich (St. Louis, MO). HBSS (10X) was purchased by Invitrogen and fatty-acid free BSA was purchased from Akron Biotech.
McCorvy J.D., Butler K.V., Kelly B., Rechsteiner K., Karpiak J., Betz R.M., Kormos B.L., Shoichet B.K., Dror R.O., Jin J, & Roth B.L. (2017). Structure-inspired design of β-arrestin-biased ligands for aminergic GPCRs. Nature chemical biology, 14(2), 126-134.
+ Open protocol
+ Expand
2

Synthesis and Characterization of Neuroactive Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
All compounds and aripiprazole were synthesized as described under General Chemistry Procedures. Dopamine hydrochloride, (−)-quinpirole, (+)-butaclamol hydrochloride, 5-hydroxytryptamine creatine sulfate, (−)-isoproterenol bitartrate, and HEPES sodium salt were purchased from Sigma-Aldrich (St. Louis, MO). HBSS (10X) was purchased by Invitrogen and fatty-acid free BSA was purchased from Akron Biotech.
McCorvy J.D., Butler K.V., Kelly B., Rechsteiner K., Karpiak J., Betz R.M., Kormos B.L., Shoichet B.K., Dror R.O., Jin J, & Roth B.L. (2017). Structure-inspired design of β-arrestin-biased ligands for aminergic GPCRs. Nature chemical biology, 14(2), 126-134.
+ Open protocol
+ Expand
3

Manipulating Dopamine Signaling in Drosophila

Check if the same lab product or an alternative is used in the 5 most similar protocols
To influence dopamine signaling, 2 days old radish1 or CS flies were put for 14h on 5mg of Methylphenidate hydrochloride (Sigma) mixed under 10ml of regular food. To inhibit dDAT or DopR1 function, we kept 2 days old CS flies for 14h on 10ml of regular food with 30mg Desipramine hydrochloride or 1mg (+)-Butaclamol hydrochloride (Sigma), respectively. For inhibition of dopamine synthesis, freshly hatched CS flies were put on 20ml of regular food with 8mg α-Methyl-DL-tyrosine (Sigma) for 120h. We verified uptake of food by the addition of a non-hazardous blue dye, which after 14h stained the abdomen of the flies. To ablate the mushroom bodies, CS flies were treated with Hydroxyurea (Sigma) [16 (link)]. Dopamine was measured by HPLC (M. Krischke, Botany Department, University of Wuerzburg).
Koenig S., Wolf R, & Heisenberg M. (2016). Visual Attention in Flies—Dopamine in the Mushroom Bodies Mediates the After-Effect of Cueing. PLoS ONE, 11(8), e0161412.
+ Open protocol
+ Expand
4

Synthesis and Radiolabeling of Sigma-1 Receptor Ligands

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
8-triazaspiro(4.5)decan-4-one,8-(3-(p-fluorobenzoyl)propyl)-1-phenyl-3 hydrochloride (spiperone), 1H-Benzo(6,7)cyclohepta(1,2,3-de)pyrido(2,1-a)isoquinolin-3-ol, 2,3,4,4a,8,9,13b,14-octahydro-3-(1,1-dimethylethyl)-(3S-(3-alpha,4a-alpha,13b-beta)) ((+)-butaclamol) hydrochloride and S-(−)-3-chloro-5-ethyl-N-[(1-ethyl-2-pyrrolidinyl)methyl]-6-hydroxy-2-methoxybenzamide hydrochloride (eticlopride), were purchased from Sigma-Aldrich. The synthesis and purification of the SV series of compounds, including 1-((5-methoxy-1H-indol-3-yl)methyl)-4-(4-(methylthio)phenyl)piperidin-4-ol (SV 293), have been previously reported (Vangveravong et al., 2006 (link); Vangveravong et al., 2011). The synthesis and purification of the 4-(dimethylamino)-N-(4-(4-(2-methoxyphenyl)piperazin-1-yl)butyl)benzamide (WC-10) and N-[(4-[4-[2-(2-fluoroethoxy)phenyl]-1-piperazinyl]butyl]-4-(3-thienyl)benzamide (LS-3-134) have been previously reported (Tu et al., 2011a (link)).
LS-3-134 was radiolabeled with tritium ([3H]LS-3-134) by American Radiolabeled Chemicals, Inc. (St. Louis, MO) to a specific activity of 60 Ci/mmol. [125I]IABN was prepared and purified as previously described (Luedtke et al., 2000 (link)). [3H]raclopride was obtained commercially (NEN-PerkinElmer).
Rangel-Barajas C., Malik M., Taylor M., Neve K.A., Mach R.H, & Luedtke R.R. (2014). Characterization of [3H]LS-3-134, a Novel Arylamide Phenylpiperazine D3 Dopamine Receptor Selective Radioligand. Journal of neurochemistry, 131(4), 418-431.
+ Open protocol
+ Expand
5

Neurotransmitter Receptor Antagonists in Fly

Check if the same lab product or an alternative is used in the 5 most similar protocols
Picrotoxin (Sigma), CGP54626 hydrochloride (Tocris Bioscience), mecamylamine hydrochloride (Sigma-Aldrich), (+)-MK-801 hydrogen maleate (Sigma-Aldrich), and (+)-butaclamol hydrochloride (Sigma-Aldrich) were used as receptor antagonists of GABAA, GABAB, nicotinic acetylcholine, NMDA, and dopamine, respectively. Picrotoxin, mecamylamine hydrochloride, and (+)-MK-801 hydrogen maleate were dissolved in saline to 500 μM, 200 μM, and 200 μM, respectively. Both CGP54626 hydrochloride and (+)-butaclamol hydrochloride were dissolved in dimethyl sulfoxide (DMSO) to 100 mM and further diluted in saline to 100 μM. These drug solutions were stored at -30°C until use. During drug application, 1 mL of the saline applied on the fixed fly was first discarded and then equivalent amounts of drug solutions or saline as control were applied. Final concentrations of Picrotoxin, CGP54626 hydrochloride, mecamylamine hydrochloride, (+)-MK-801 hydrogen maleate, and (+)-butaclamol hydrochloride were 250 μM, 50 μM, 100 μM, 100 μM, and 50 μM, respectively.
Tachibana S.I., Touhara K, & Ejima A. (2015). Modification of Male Courtship Motivation by Olfactory Habituation via the GABAA Receptor in Drosophila melanogaster. PLoS ONE, 10(8), e0135186.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!