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Pd 184352

Manufactured by Cell Signaling Technology
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Sourced in Germany

PD-184352 is a potent and selective inhibitor of the mitogen-activated protein kinase kinase (MEK1/2). It functions by blocking the activation of ERK1/2, which are key components of the Ras/Raf/MEK/ERK signaling pathway.

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Lab products found in correlation

Peg sod, by Merck Group (1 mentions) Valsartan, by Merck Group (1 mentions) Cgs35066, by Bio-Techne (1 mentions) Y 27632, by Merck Group (1 mentions) Selumetinib, by Selleck Chemicals (1 mentions) Gsk269962, by Bio-Techne (1 mentions) Gdc 0994, by Selleck Chemicals (1 mentions) Zstk474, by Selleck Chemicals (1 mentions)

2 protocols using pd 184352

1

Inhibitor Evaluation of Cardiovascular Markers

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Unless stated otherwise, all reagents were from Merck (Darmstadt, Germany). Inhibitors used were: CGS35066, an ECE-1 inhibitor (Tocris Bioscience/Bio-Techne; Wiesbaden, Germany); valsartan, an angiotensin receptor type I (AT1) antagonist (Sigma-Aldrich; Schnelldorf, Germany, Merck, Darmstadt, Germany); PD-184352, an inhibitor of extracellular signal-regulated kinases (ERK) (Cell Signaling Technology, Danvers, MA, USA); and PEG-SOD, a superoxide dismutase-polyethylene glycol conjugate (Sigma-Aldrich®, Merck). Their effective concentrations were determined in preliminary experiments.
Adu-Gyamfi M., Goettsch C., Kamhieh-Milz J., Chen L., Pfefferkorn A.M., Hofmann A., Brunssen C., Müller G., Walther T., Ashraf M.I., Morawietz H., Witowski J, & Catar R. (2024). The Role of NOX2-Derived Reactive Oxygen Species in the Induction of Endothelin-Converting Enzyme-1 by Angiotensin II. Antioxidants, 13(4), 500.
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2

Small Molecule Inhibitors in Cell Signaling

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The following small molecule inhibitors were used in this study: MEK inhibitors: 2 μM PD 184352 (Cell Signaling 12147) and 10 μM Selumetinib (Selleckchem S1008), ERK inhibitor: 10 μM GDC-0994 (Selleckchem S755403), PI3k inhibitor: 2 μM ZSTK474 (Selleckchem S1072), ROCK inhibitors: 25 μM Y-27632 (Sigma Y0503) and 2 μM GSK 269962 (Tocris 4009). Details of treatment times are described in figure legends.
Matthews H.K., Ganguli S., Plak K., Taubenberger A.V., Win Z., Williamson M., Piel M., Guck J, & Baum B. (2020). Oncogenic Signaling Alters Cell Shape and Mechanics to Facilitate Cell Division under Confinement. Developmental Cell, 52(5), 563-573.e3.
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