Reagents for sds page electrophoresis

Antibodies utilized in this study include anti-CD9 (Santa Cruz Biotechnology Inc., Dallas, TX), anti-CD63 (Abcam, Cambridge, MA), anti-CD81 (GeneTex Inc., Irvine, CA), anti-AHNAK 1 (Thermo Scientific, Waltham, MA), anti-HABP2 (Abnova, Walnut, CA), anti-CD44 (IM7 clone) (BD Biosciences), anti-CD44v10 (Novus Biologicals), and anti-actin (Sigma). Hyaluronic acid (sodium salt from Streptococcus zooepidemicus), LPS, ATP, and ionomycin were purchased from Sigma. Reagents for SDS-PAGE electrophoresis were purchased from Bio-Rad (Richmond, CA) and Immobilon-P transfer membrane was purchased from Millipore (Millipore Corp., Bedford, MA).

Human NSCLC cell lines A549, SK-LU-1, H1703, H358, H1993, H661, SW1573, H522, H226, H1437, H1838, H1975, H2170, and non-cancerous BEAS-2B were obtained from ATCC (Walkersville, MD, USA) and cultured in Roswell Park Memorial Institute complete medium (Cambrex, East Rutherford, NJ, USA) at 37°C in a humidified atmosphere of 5% CO₂, 95% air, with passages 6–10 used for experimentation. Unless otherwise specified, reagents were obtained from Sigma (St. Louis, MO, USA). UK122 was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Reagents for SDS-PAGE electrophoresis were purchased from Bio-Rad (Richmond, CA, USA) and Immobilon-P transfer membrane was purchased from Millipore (Millipore Corp., Bedford, MA, USA). Mouse anti-HABP2 antibody was purchased from Novus Biologicals (Littleton, CO, USA). Mouse anti-human nestin antibody that does not react with mouse or rat nestin (clone 10C2) was purchased from Millipore (Bedford, MA, USA). Mouse anti-β-actin antibody was purchased from Sigma (St. Louis, MO, USA). Secondary horseradish peroxidase-labeled antibodies were purchased from Amersham Biosciences (Piscataway, NJ, USA).