Si igfbp 3

Manufactured by GenePharma

Sourced in China

Si-IGFBP-3 is a laboratory reagent that is used to detect and quantify the presence of insulin-like growth factor binding protein-3 (IGFBP-3) in biological samples. IGFBP-3 is a protein that binds to insulin-like growth factors and plays a role in regulating their bioavailability and activity.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Mir nc, by GenePharma (1 mentions) Pcdna3.1 vector, by Sangon (1 mentions)

2 protocols using si igfbp 3

Transfection of Hep3B Cells with IGFBP-3

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Hep3B cells were transfected with pcDNA3.1-IGFBP-3 and si-IGFBP-3, and the siRNA sequence was designed and synthesized by GenePharma (GenePharma, Shanghai, China) (

Supplementary Table 1

). Cells were transfected with pcDNA3.1-IGFBP-3 and IGFBP-3 siRNA plus Lipofectamine 2000 (Invitrogen, California, USA). All operations were carried out in accordance with the manual for instructions.

Wang H., Wang H., Li K., Li S, & Sun B. (2020). IGFBP-3 Is the Key Target of Sanguinarine in Promoting Apoptosis in Hepatocellular Carcinoma. Cancer Management and Research, 12, 1007-1015.

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Modulating miR-19a-3p and IGFBP3 in SH-SY5Y cells

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The miR-19a-3p inhibitor (inhibitor) and its negative control (miR-NC) were synthesized by GenePharma Co., Ltd. (Shanghai, China). The pcDNA3.1/IGFBP3 vector was generated by inserting the open reading frame of IGFBP3 without 3′UTR into the pcDNA3.1 vector (Sangon Biotech, Shanghai, China). Small interference RNA against IFGBP3 (si-IGFBP3) was purchased from GenePharma Co., Ltd. Before OGD/R, SH-SY5Y cells were seeded at a density of 3 × 10⁵ cells per well and transfected with inhibitor, miR-NC, IGFBP3 or empty vector. In the rescue experiments, si-IGFBP3 was transfected into miR-19a-3p silenced SH-SY5Y cells. All these transfection procedures were performed for 48 h using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s protocols.

Chai Z., Gong J., Zheng P, & Zheng J. (2020). Inhibition of miR-19a-3p decreases cerebral ischemia/reperfusion injury by targeting IGFBP3 in vivo and in vitro. Biological Research, 53, 17.

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