HT1080 cells (KCLB No. 10121, human fibrosarcoma), PC-3 cells (KCLB No. 21435, human prostate adenocarcinoma), and B16F10 cells (KCLB No. 80008, murine melanoma) were obtained from Korean Cell Line Bank (Seoul, Korea) in 2012, where they were characterized by DNA fingerprinting. Cells were maintained in RPMI1640 or Dulbecco's modified Eagle's medium (DMEM, Cellgro, Manassas, VA) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS; Cellgro) and 100 U/ml penicillin/100 μg/ml streptomycin (Cellgro) at 37°C in a humidified 5% CO2 incubator. Human umbilical vein endothelial cells (HUVECs) purchased from Innopharmascreen (Asan, Korea) in October 2012 were maintained in Endothelial cell Growth Medium-2 (EGM-2; PromoCell, Heidelberg, Germany) and used at passages 3 to 8. Cells were routinely screened for Mycoplasma contamination.
Ht1080 cells
Manufactured by Korean Cell Line Bank
Sourced in United States
HT1080 cells are a human fibrosarcoma cell line. They are adherent cells derived from a fibrosarcoma tissue of a 35-year-old Caucasian male. HT1080 cells express various proteins and receptors and can be used for various cell-based assays and experiments.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Corning (2 mentions)
Streptomycin, by Corning (2 mentions)
Penicillin, by Corning (2 mentions)
Endothelial cell growth medium 2 egm2, by PromoCell (2 mentions)
B16f10 cells, by Korean Cell Line Bank (2 mentions)
Rpmi 1640, by Corning (1 mentions)
Pc 3 cells, by Korean Cell Line Bank (1 mentions)
Mda mb 231, by Korean Cell Line Bank (1 mentions)
Du145, by Korean Cell Line Bank (1 mentions)
Egm 2, by PromoCell (1 mentions)
Penicillin streptomycin, by Corning (1 mentions)
Fetal bovine serum (fbs), by Cytiva (1 mentions)
Rpmi 1640 media, by Cytiva (1 mentions)
3 protocols using ht1080 cells
1
Cell Culture Protocols for Various Cell Lines
2
Cell Culture Protocols for Cancer Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human fibrosarcoma HT1080 cells (KCLB No. 10121), human breast adenocarcinoma MDA-MB231 (KCLB No. 30026), human prostate carcinoma DU145 (KCLB No. 30081), and murine melanoma B16F10 cells (KCLB No. 80008) were purchased from Korean Cell Line Bank (Seoul, Korea). Cells were grown in Roswell Park Memorial Institute (RPMI) 1640 or Dulbecco’s modified Eagle’s medium (DMEM; Lonza, Walkersville, MD, USA) supplemented with 10% FBS (Cellgro, Manassas, VA, USA) and antibiotics (100 U/ml penicillin/100 μg/ml streptomycin; Cellgro) at 37 °C in a humidified 5% CO2 incubator. HUVECs obtained from Innopharmascreen (Asan, Korea) were maintained in EGM-2 (PromoCell, Heidelberg, Germany) and used for experiments at passages 3 to 8.
3
HT1080 Fibrosarcoma and HUVEC Culture
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Human fibrosarcoma HT1080 cells were obtained from the Korean Cell Line Bank (KCLB, No. 10121) and maintained in RPMI1640 media (Hyclone Laboratories, Logan, UT, United States) with 10% fetal bovine serum (FBS, Hyclone Laboratories) and penicillin/streptomycin (Cellgro, Manassas, VA, United States) at 37°C in a humidified 5% CO2 incubator. Human umbilical vein endothelial cells (HUVECs) were obtained from Innopharmascreen (Asan, Republic of Korea), maintained in Endothelial Cell Growth Medium-2 (EGM-2, PromoCell, Heidelberg, Germany), and used for assays at passages 3–8.
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