Anti hla dr pe cy5

The following antibodies were used for flow cytometric analysis: Anti-Perforin-Alexa fluor 488, anti-CD28-allophycocyanin (APC), anti-CD19-APC, anti-CD3-APC, anti-CD56-APC, anti-CD3-APC-cyanin7 (Cy7), anti-CD8-APC-Cy7, anti-CD14-APC-Cy7, anti-CD14-fluorescein isothiocyanate (FITC), anti-HLA-DR-FITC, anti-CD16-R-phycoerythrin (PE), anti-GATA3-PE, anti-CD45RA-PE-cyanin 5 (Cy5), anti-CD4-PE-Cy5, anti-CD16-PE-Cy5, anti-CCR7-PE-Cy7, anti-CD4-PE-Cy7, anti-CD4-V450, anti-GranzymeB-V450, anti-CD8-V500, anti-CD3-V500 (all from BD Bioscience, Franklin Lakes, NJ), anti-CD57-FITC, anti-CD4-FITC, anti-CD85j-PE, anti-CX3CR1-PE, anti-T-bet-PE-Cy7, anti-Eomes-Peridinin chlorophyll (PerCP)-efluor710 (six from eBioscience, San Diego, CA), anti-HLA-DR-PE-Cy5, anti-IL-7Rα-V450, anti-CD57-V450 (three from BioLegend, San Diego, CA), anti-CX3CR1-FITC (MBL International Corporation, Woburn, MA). For intracellular staining of T cell lineage-specific transcription factors (T-bet, Gata3, and Eomes), granzyme B and perforin, PBMC were fixed and permeabilized with Fix/Perm buffer set (BioLegend). Stained cells were acquired by a BD LSRFortessa (BD bioscience) and analyzed by using FlowJo software (ver. 9.0 or 10.0; Tree Star, OR).

Zombie UV™ Fixable Viability Kit, anti-CD45 BV570, anti-CD3 BV605, anti-CD4 BV421, anti-CD8 BV650, anti-CD4 APC-Fire750, anti-CD73 PE/Dazzle 594, anti-CD56 PE, anti-CD14 PerCP/Cy5.5, anti-CD16 Alex Fluor 700, anti-CD19 APC-Fire750, anti-HLA-DR PE-Cy5, anti-PD-1 BV421, anti-CD16 PE, anti-IFNγ A700, anti-IL-13 APC, anti-IL-5 APC, anti-TNFα Alexa Fluor 488, and anti-IL-21 PE were purchased from BioLegend (London, UK). Anti-CD25 BB515, anti-CD4 BUV496, anti-CD39 BV711, anti-Perforin Alexa Fluor 647, anti-Granzyme B Alexa Fluor 700, anti-Ki-67 Alexa Fluor 700, anti-CD45RA PE, anti-IL-2 PerCP/Cy5.5, and Fixation/Permeabilization Solution Kit were purchased from Becton Dickinson. Anti-FOXP3 APC, anti-CD56 APC-eFluor780, and eBioscience™ (San Diego, CA, USA) Foxp3/Transcription Factor Staining Buffer Set were purchased from eBioscience TermoFisher scientific. Anti-TIM-3 APC was purchased from R&D systems. Anti-CD137 PE and FcR blocking reagent were purchased from Miltenyi (Bergisch Gladbach, Germany).
Peptides for the PSA, PSMA, PSCA, and PAP pools (Supplementary Table S1) were synthetized and lyophilized by the Peptide and Tetramer Core Facility of the Department of Oncology at UNIL-CHUV (Lausanne, Switzerland), and 5T4 pools (Supplementary Tables S2 and S3) were synthetized and lyophilized by JPT Peptide Technologies.