Eight tea catechin derivatives – (+)-catechin(C), (−)-epicatechin (EC), (−)-gallocatechin (GC), (−)-epigallocatechin (EGC), (+)-catechin gallate (CG), (−)-epicatechin gallate (ECG), (−)-gallocatechin gallate (GCG), and (−)-epigallocatechin gallate (EGCG) – were purchased from Sigma (St. Louis, MO, USA). The compounds, purified using the HPLC system, were of analytical grade. We purchased 2,2′-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,4,6-tripyridyl-s-triazine (TPTZ), and all other chemicals from Sigma Aldrich (St. Louis, MO, USA) and all were of analytical grade.
Epicatechin gallate ecg
Manufactured by Merck Group
Sourced in United States
(-)-Epicatechin gallate (ECG) is a polyphenolic compound that is obtained from green tea and other plant sources. It is a laboratory standard used in chemical analysis and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Epigallocatechin gallate (egcg), by Merck Group (4 mentions)
Catechin gallate, by Merck Group (2 mentions)
Gallocatechin gallate gcg, by Merck Group (2 mentions)
Epigallocatechin egc, by Merck Group (2 mentions)
2 2 azino bis, by Merck Group (1 mentions)
1 1 diphenyl 2 picrylhydrazyl dpph, by Merck Group (1 mentions)
2 4 6 tripyridyl s triazine tptz, by Merck Group (1 mentions)
Diammonium salt, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
Kaempferol, by Merck Group (1 mentions)
Cell counting kit 8 (cck8), by Dojindo Laboratories (1 mentions)
Erm cz100, by Merck Group (1 mentions)
Myricetin, by Merck Group (1 mentions)
2 7 dichlorodihydrofluorescein diacetate dcfh da, by Merck Group (1 mentions)
Mitosox red m36008, by Thermo Fisher Scientific (1 mentions)
Mtt assay kit, by Dojindo Laboratories (1 mentions)
Theaflavin 3 gallate, by Merck Group (1 mentions)
Theaflavin, by Merck Group (1 mentions)
4 protocols using epicatechin gallate ecg
1
Purification and Antioxidant Evaluation of Tea Catechins
2
Antioxidant Potential of Green Tea Compounds
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The airborne FDPs reference material ERM-CZ100 (smaller than 10 μm) and the pure catechin compounds EGCG, epigallocatechin (EGC), epicatechin (EC), and epicatechin gallate (ECG) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Phenolic compounds, quercetin, kaempferol, and myricetin were also obtained from Sigma-Aldrich. Dried green tea leaves (Osulloc Farm, Jeju Island, Korea) were obtained, extracted, and purified for the preparation of GTE, FLGs, and CTPs. All other chemicals used in this study were of analytical grade.
ERM-CZ100 was suspended in serum-free DMEM and homogenized by sonication to make a 10 mg/ml stock solution. Phosphate-buffered saline (PBS), dimethyl sulfoxide (DMSO), and the fluorescent probe 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) were purchased from Sigma-Aldrich. MitoSOX Red (M36008) was obtained from Thermo Fisher Scientific (Indianapolis, IN, USA), and an MTT assay kit and a Cell Counting Kit-8 were purchased from Dojindo (Kumamoto, Japan).
ERM-CZ100 was suspended in serum-free DMEM and homogenized by sonication to make a 10 mg/ml stock solution. Phosphate-buffered saline (PBS), dimethyl sulfoxide (DMSO), and the fluorescent probe 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) were purchased from Sigma-Aldrich. MitoSOX Red (M36008) was obtained from Thermo Fisher Scientific (Indianapolis, IN, USA), and an MTT assay kit and a Cell Counting Kit-8 were purchased from Dojindo (Kumamoto, Japan).
3
Tea Catechins and Theaflavins Extraction
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The catechins including (−)-gallocatechin (GC), (−)-epigallocatechin (EGC), (+)-catechin (C), (−)-epicatechin (EC), (−)-epigallocatechin gallate (EGCG), (−)-gallocatechin gallate (GCG), (−)-epicatechin gallate (ECG), (−)-catechin gallate (CG), caffeine, l -theanine and eighteen amino acid standards (kit) were purchased from Sigma (St. Louis, MO, USA). Four theaflavin monomers including theaflavin (TF1, 95%), theaflavin-3-gallate (TF2A, 92%), theaflavin-3′-gallate (TF2B, 90%) and theaflavin-3, 3′-digallate (TF3, 90%) were prepared according to the method developed in our lab, and their purities were determined by high-performance liquid chromatography-ultraviolet-mass spectrum (HPLC-UV-MS), with the standard samples from sigma company.12 (link) Methanol and acetonitrile of HPLC grade were purchased from Tianjin Shield Company (Tianjin, China). Other reagents of analytical reagent grade were purchased from National Medicine Chemical Reagent Co., Ltd. (Shanghai, China).
4
Polyphenol Characterization and Preparation
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Figure 1 shows the structure of the 19 polyphenols included in this study. Quercetin dihydrate (≥98%), morin hydrate, (±)-naringenin (~95% ), galangin, apigenin (≥95%), rutin hydrate (≥94%), chrysin (97%), (-)-epigallocatechin gallate [(-)-EGCG] (≥95%), (-)-epigallocatechin [(-)-EGC] (≥95%), (-)-epicatechin gallate [(-)-ECG] (≥95%), (-)-epicatechin [(-)-EC] (≥95%) and gallic acid monohydrate [GA] (≥99%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Myricetin, dihydroMyricetin (both ≥95%) and kaempferide were obtained from Extrasynthese (Genay, France). KNO 3 and absolute ethanol (both pro analysis) were purchased from Kemika (Zagreb, Croatia). Buffer solution pH 3 was obtained from Reagecon (Shannon, Co. Clare, Ireland). Water was deionised by the Millipore Milli-Q system to the resistivity ≥18 MΩ cm.
Stock standard solutions of polyphenols (1.0x10 -2 mol L -1 ) were prepared from the dry pure substances. The stock solutions of catechins (EGCG, EGC, ECG, and EC) and gallic acid were prepared in deionised water obtained from a Millipore Milli-Q purification system. All other flavonoid stock standard solutions were prepared in absolute ethanol. The stock solutions were protected from light with aluminium foil and kept in a refrigerator.
Stock standard solutions of polyphenols (1.0x10 -2 mol L -1 ) were prepared from the dry pure substances. The stock solutions of catechins (EGCG, EGC, ECG, and EC) and gallic acid were prepared in deionised water obtained from a Millipore Milli-Q purification system. All other flavonoid stock standard solutions were prepared in absolute ethanol. The stock solutions were protected from light with aluminium foil and kept in a refrigerator.
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