Ft ir 670 plus

FTIR studies were carried out in a FT/IR-670 plus (Jasco, Tokyo, Japan) to detect interaction of each component before and after nanoparticulation. Sb, chitosan, PLGA, pluronic and the nanoparticles were pelletized individually with IR grade KBr in the ratio of 1∶100 in a hydraulic press at a pressure of 150 bar for 30 sec. The pellets were scanned over a range of 4000 to 400 cm⁻¹ at a resolution of 4 cm⁻¹ and the data stacked in Biorad KnowItAll software for analysis and overlap regions.

Reagents used for synthesis were purchased from Sigma-Aldrich (Munich, Germany) and Across Organics and were used without further purification.
The melting points were determined on a Boetius apparatus (Jena, Germany) and are uncorrected.
An AmaZon EDT apparatus (Bremen, Germany) equipped with an electrospray ion source and an ion trap analyzer was used to perform the mass spectrometry analysis. The samples were introduced into the apparatus after dissolving them in a mixture of methanol and chloroform (1: 1 v/v) with the addition of 0.1% vol of formic acid. The scanning was carried out in the range of 100–700 m/z, accumulating approximately 300,000 ions in one cycle. Positive or negative ion polarization was used, depending on the properties of the tested sample.
The IR spectra were recorded on a JASCO FT IR-670 Plus instrument in KBr pellets (1 mg sample/400 mg KBr).
The ¹H-NMR spectra and ¹³C-NMR spectra were recorded on a JEOL JNM-ECZR500 RS1 (ECZR version) at 500 and 126 MHz, respectively, and were reported in parts per million (ppm) using deuterated solvent for calibration (DMSO-d6). The coupling constants values (J) were reported in hertz (Hz), and the splitting patterns were designated as follows: br s. (broad singlet), br d. (broad doublet), s (singlet), d (doublet), t (triplet), q (quartet), dd (doublet of doublets), m (multiplet).