Percp anti mouse cd11b

Manufactured by BioLegend

PerCP anti-mouse CD11b is a fluorescently-labeled antibody that binds to the CD11b surface antigen expressed on mouse myeloid cells, including monocytes, granulocytes, and macrophages.

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Lab products found in correlation

Fitc anti mouse cd206, by BioLegend (1 mentions) Fitc anti mouse cd206 mmr, by BioLegend (1 mentions) Percp anti mouse f4 80, by BioLegend (1 mentions) Pe anti mouse cd11b, by BD (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Bv510 anti mouse cd45 antibody, by BioLegend (1 mentions) Anti mouse cd3 fitc, by Thermo Fisher Scientific (1 mentions) Bv421 anti mouse nk1, by BioLegend (1 mentions) Anti mouse f4 80 apc, by Thermo Fisher Scientific (1 mentions) Apc anti mouse cd8a, by Thermo Fisher Scientific (1 mentions) Facsaria 2 cell sorter, by BD (1 mentions) Anti mouse cd206 bv421, by BioLegend (1 mentions) Facsaria 2 flow cytometer, by BD (1 mentions)

2 protocols using percp anti mouse cd11b

Characterizing Retinal and Choroidal Macrophages

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To examine macrophages in the retina and choroid, cells were prepared from mouse eyes. To collect a sufficient number of ocular in ltrating cells, 50 burns were delivered to mouse eyes by laser. After laser injury, eyes were enucleated at different time points (1, 2, 3, 5, and 7days). The anterior segment (cornea, iris, and lens) was excised and the posterior segment of the eye including sclera, choroid, and retina was disrupted with scissors and then shaken in DMEM (plus 10% FBS (Gibco Laboratories), 100U/ml penicillin, 100μg/ml streptomycin) supplemented with 0.5mg/ml Collagenase type D (11088874103, Boehringer Mannheim) at 37°C for 60min. The supernatants were collected and passed through a mesh. After 3 washes, viable cells were obtained. A total of 12 eyes (6 individual pools) were examined per group. The cells were stained with PE anti-mouse CD11b (557397, BD Pharmingen), FITC anti-mouse CD206 (MMR, 123005; BioLegend) and PE-Cy5 anti-mouse CD80 Abs (15-0801-81, eBioscience).
RAW 264.7 cells were stained with PerCP anti-mouse CD11b (101230, BioLegend), PerCP anti-mouse F4/80 (123006, BioLegend), PE anti-mouse CD80 (12-0801, eBioscience), and FITC anti-mouse CD206 (141704, BioLegend).

Zandi S., Nakao S., Chun K.H., Fiorina P., Sun D., Arita R., Zhao M., Kim E., Schueller O., Campbell S., Taher M., Melhorn M.I., Schering A., Gatti F., Tezza S., Xie F., Vergani A., Yoshida S., Ishikawa K., Yamaguchi M., Sasaki F., Schmidt-Ullrich R., Hata Y., Enaida H., Yuzawa M., Yokomizo T., Kim Y.B., Sweetnam P., Ishibashi T, & Hafezi-Moghadam A. (2015). ROCK-Isoform Specific Polarization of Macrophages Associated with Age-Related Macular Degeneration. Cell reports, 10(7), 1173-1186.

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Multicolor Flow Cytometry Immunophenotyping

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Cells were incubated with Fc block antibody (#14-0161-82, eBioscience, 1:100) for 30 min at 4 °C to prevent nonspecific binding. Cell surface labeling was conducted with fluorescently conjugated antibodies for 30 min at 4°C. The following antibodies were used: BV510 anti-mouse CD45 antibody (#103137, Biolegend, 1:25), PerCP anti-mouse CD11b (#101229, Biolegend, 1:50), APC anti-mouse F4/80 (#17-4801-80, eBioscience, 1:25), PE/Cy7 anti-mouse Gr1 (#108415, Biolegend, 1:100), FITC anti-mouse CD3 (#11-0032-82, eBioscience, 1:50), PE/Cy7 anti-mouse CD19 (#25-0193-81, eBioscience, 1:50), BV421 anti-mouse NK1.1 (#108741, Biolegend, 1:20), PE anti-mouse CD36 (#562702, BD Biosciences, 1:100), PE anti-mouse CD4 (#4329629, Invitrogen, 1:200), APC anti-mouse CD8a (#17-0081-81, eBioscience, 1:200), BV421 anti-mouse CD206 (#141717, Biolegend, 1:25), FITC anti-mouse CD80 (#FITC-65076, Proteintech, 1:200), FITC anti-mouse GzmB (#372206, Biolegend, 1:25), FE anti-mouse IFNγ (#PE-65153, Proteintech, 1:50). Fluorescence data were collected using a FACSAria II flow cytometer (BD Biosciences, USA) and analyzed employing a FlowJo software. The single cell population was separated with a BD FACSAria II Cell Sorter.

Yang P., Qin H., Li Y., Xiao A., Zheng E., Zeng H., Su C., Luo X., Lu Q., Liao M., Zhao L., Wei L., Varghese Z., Moorhead J.F., Chen Y, & Ruan X.Z. (2022). CD36-mediated metabolic crosstalk between tumor cells and macrophages affects liver metastasis. Nature Communications, 13, 5782.

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