Fatp 4

Cells were plated on glass coverslips, fixed with 4% paraformaldehyde (PFA) and incubated overnight at 4°C with primary antibodies against CD36 (rabbit, HPA002018, 1:200, Sigma-Aldrich), FABPpm (mouse, 1:200, Novus Biologicals) FATP1 (mouse, 1:200, Novus Biologicals), FATP-4 (mouse, 1:200, Abnova). For plasma membrane labeling, Alexa Fluor 594 wheat germ agglutinin conjugate (W11262, Molecular Probes, Eugene, OR) was used. Samples were stained with Alexa Fluor 488 (A11059, A11001 and A11008, 1:2,000, Molecular Probes) conjugated secondary antibodies. After nuclei counterstain with Hoechst, coverslips were imaged with an inverted laser scanning confocal microscope (Zeiss LSM 510 equipped with a 63× oil immersion objective, Thornwood, NY). Z-stack confocal images were taken at 30-s intervals using AIM (version 3.2, Zeiss), and images were compiled using Zeiss LSM Image Browser (versions, 4.2, Zeiss). For epifluorescent microscopy, images were taken with a Zeiss Axiovert 100 microscope.