Cal 148

MDA-MB-453, T-47D, 22Rv1, VCaP, ZR75–1, MDA-MB-231, MCF7 and HEK293T/17 cells were obtained from the American Type Culture Collection (Manassas, VA, USA). MFM-223, Cal-51 and Cal-148 cells were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany). LNCaP-95 cells were a kind gift from Dr. Alan K. Meeker (Johns Hopkins University). MDA-MB-453, T-47D, ZR75–1 and 22Rv1 cells were maintained in RPMI-1640 medium containing 10% fetal bovine serum (FBS); VCaP cells were maintained in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% FBS, 1% sodium pyruvate, 1% MEM non-essential amino acids and 0.1 nM 5α-dihydrotestosterone (DHT; Sigma, St. Louis, MO, USA); MDA-MB-231 cells were maintained in RPMI-1640 medium containing 5% FBS; MCF7 cells were maintained in Eagle's Minimum Essential Medium (EMEM) containing 10% FBS, 2 mM L-glutamine, sodium pyruvate and 0.2 U/mL insulin; MFM-223 cells were maintained in EMEM containing 10% FBS, 2 mM L-glutamine, and insulin-transferrin-sodium selenite (ITS) supplement; Cal-51 cells were maintained in DMEM containing 10% FBS; Cal-148 cells were maintained in DMEM containing 10%FBS, 2 mM L-Glutamine and EGF (1ug/100mL); HEK293T/17 cells were maintained in DMEM containing 10% FBS and 20 mM HEPES.