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Prism 6.0g

Manufactured by GraphPad
Sourced in United States

Prism 6.0g is a data analysis and graph-making software developed by GraphPad. It is designed to help researchers analyze, visualize, and present scientific data.

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14 protocols using prism 6.0g

1

Statistical Analysis of Experimental Data

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We calculated significance by one-way ANOVA with Bonferroni or Tukey post hoc testing, Mann-Whitney U test or Student t test, as appropriate, using Prism 6.0g (GraphPad; La Jolla, CA) on an Intel iMac computer. Results were considered significant at p <0.05.
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2

Evaluating Chronic Intermittent Ethanol Effects

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For the CIE-2BC experiments, a four-way ANOVA was performed for CIE episodes (CIE1-4) and treatments (air or vapor) with between subjects factors of genotype, sex and treatment, and the within-subjects factor of CIE episode. Data was further analyzed using two-way ANOVA for each genotype and CIE episode with the between subjects factors of treatment and sex using Prism 6.0g software for Mac (GraphPad, San Diego, CA, USA). For the electrophysiology experiments, MiniAnalysis 5.1 software was used for data analysis and Prism 5.0 software (GraphPad) was used for statistical analyses. The electrophysiology results were evaluated with cumulative probability analysis, and significance determined with the Kolmogorov-Smirnov nonparametric two-sample test. T-test analyses were used for individual means comparisons, and within-subject one-way repeated measures ANOVAs were used to compare sIPSCs within a group. When appropriate, the Bonferroni post hoc comparisons were used to assess significance between treatments. Data are presented as the mean ± SEM.
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3

Statistical Analysis of Experimental Data

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Graph Pad Prism 6.0g was used for statistical analysis of the data. For the statistical analysis of two groups the Mann-Whitney U test was executed. Column based data containing more than 2 groups was compared using One-way ANOVA. To compare two groups at different time points multiple t-tests were performed. For all multiple testing scenarios, the reported p-values were adjusted accordingly. Correlations were performed using the Spearman correlation method. Survival data was statistically analyzed with the Mantel-Cox log-rank test.
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4

Estimating Wolbachia-Mediated Infection Rate

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In each experiment, the maximum likelihood estimate (MLE) of infection rate per 1000 larvae was calculated independently for -Wolb and +Wolb mosquitoes using the formula: MLE=11Y/X1/m
where Y is the number of positive pools, X is the total number of pools, and m is the average pool size in the experiment [20 (link)].
Minimum infection rates (MIR) were not calculated due to non-uniformity in pool sizes. MLEs were also compiled across all injection data, and compared between -Wolb and +Wolb mosquitoes using Fisher’s exact test.
DENV prevalence data (proportion of mosquitoes infected) were compared between -Wolb and +Wolb mosquitoes using Fisher’s exact test, while DENV load data (DENV titre) were not normally distributed, and were compared using Mann-Whitney U-tests. All statistical analyses were performed using Prism 6.0 g (Graphpad).
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5

Nonparametric Statistical Analyses

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Statistical analyses were performed using Prism 6.0g (GraphPad Software, Inc., San Diego, CA). Mann-Whitney U tests were used for paired nonparametric measures and Wilcoxon’s test for nonpaired nonparametric measures to determine statistical significance (P < 0.05).
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6

Comparative Analyses of Research Approaches

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All data are expressed as mean ± SD (standard deviation). We employed a paired Student's t-test for the comparison of different approaches at each step with the original data and with each other. All analyses were performed using Prism 6.0g (GraphPad Software Inc.). P < 0.05 was considered statistically significant.
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7

Statistical Analysis of Experimental Protocols

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All statistical analysis presented was performed with GraphPad Prism 6.0 g (La Jolla, CA). Unless indicated otherwise, all experiments presented were performed at in at least three independent replicates.
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8

Statistical Analysis of Experimental Data

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Data are reported as mean±SEM from (n) independent experiments. Gaussian data were analysed using analysis of variance with Holm–Sidak correction for multiple comparisons, or paired t test where appropriate. Data that could not be assumed to be from a Gaussian population were analysed using Kruskal–Wallis with Dunn's post-test. All analyses were undertaken using Prism 6.0g software (GraphPad, San Diego, California, USA). A p value of <0.05 was considered significant, *p<0.05, **p<0.01, ***p<0.005, ****p<0.001.
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9

Statistical Analysis of Experimental Data

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Statistical analyses were carried out using GraphPad Prism 6.0 g software. Two-way ANOVA with Bonferroni’s post-test was used to compare multiple groups. Values are reported as mean ± SEM (standard error of the mean).
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10

Evaluating Parametric Assumptions and Statistical Analyses

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Prior to analysis, parametric assumptions were evaluated for all variables using histograms, identification of outliers with boxplots, descriptive statistics, and the Shapiro–Wilk test for normality. Data are reported as medians (range) if not normally distributed or means (standard deviation) if normally distributed. Student’s t-test and repeated measures analysis of variance (ANOVA) were used for normally distributed data and Friedman test and Mann Whitney U-test were used for not normally distributed data. Post-hoc analyses were conducted using Holm-Sidak correction for multiple comparisons across time after surgery for MCD for the 5CTV data using baseline data prior to surgery as control. For between group post-hoc analyses, comparisons were made for each time point, correcting the p-value by dividing 0.05 by the number of time points (50) for a final p-value of 0.001 required for significance. Analyses were carried out using OriginPro 9.5 (OriginLab, Northampton, MA) or Prism 6.0g (GraphPad Software, San Diego, CA). A p-value of 0.05 was considered significant unless otherwise noted above. There are no missing data or outliers to report and no animals were excluded from the study.
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