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Chromium single cell 3 library kit v2 and i7 multiplex kit

Manufactured by 10x Genomics
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The Chromium Single Cell 3' Library Kit V2 is a product from 10x Genomics used for generating single-cell RNA sequencing libraries. The i7 Multiplex Kit is a complementary product used for sample indexing during library preparation.

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Lab products found in correlation

Chromium single cell chip kit, by 10x Genomics (2 mentions)

Spelling variants of this product

Chromium i7 Multiplex Kit (51 citations) Chromium Single Cell 3′ Library (33 citations) I7 Multiplex Kit (26 citations) Chromium Single Cell 3′ v2 (4 citations) Chromium Single Cell 3′ v2 Library (2 citations)

2 protocols using chromium single cell 3 library kit v2 and i7 multiplex kit

1

Smart-Seq2 for Single-Cell Transcriptomics

Check if the same lab product or an alternative is used in the 5 most similar protocols
For Smart-Seq2 single cells were processed by SciLifeLab – Eukaryotic Single cell Genomic Facility (Karolinska Institute). Before shipping single cells were sorted into wells of a 384-well plate containing pre-prepared lysis buffer. The single cell solution of cells and primary human kidney cells were run in parallel on a Chromium Single Cell Chip kit and libraries were performed using Chromium Single Cell 3’ library kit V2 and i7 Multiplex kit (PN-120236, PN-120237, PN-120262, 10x Genomics) according to the manufacturer’s protocol.
Kuppe C., Ibrahim M.M., Kranz J., Zhang X., Ziegler S., Perales-Patón J., Jansen J., Reimer K.C., Smith J.R., Dobie R., Wilson-Kanamari J.R., Halder M., Xu Y., Kabgani N., Kaesler N., Klaus M., Gernhold L., Puelles V.G., Huber T.B., Boor P., Menzel S., Hoogenboezem R.M., Bindels E.M., Steffens J., Floege J., Schneider R.K., Saez-Rodriguez J., Henderson N.C, & Kramann R. (2020). Decoding myofibroblast origins in human kidney fibrosis. Nature, 589(7841), 281-286.
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2

Smart-Seq2 for Single-Cell Transcriptomics

Check if the same lab product or an alternative is used in the 5 most similar protocols
For Smart-Seq2 single cells were processed by SciLifeLab – Eukaryotic Single cell Genomic Facility (Karolinska Institute). Before shipping single cells were sorted into wells of a 384-well plate containing pre-prepared lysis buffer. The single cell solution of cells and primary human kidney cells were run in parallel on a Chromium Single Cell Chip kit and libraries were performed using Chromium Single Cell 3’ library kit V2 and i7 Multiplex kit (PN-120236, PN-120237, PN-120262, 10x Genomics) according to the manufacturer’s protocol.
Kuppe C., Ibrahim M.M., Kranz J., Zhang X., Ziegler S., Perales-Patón J., Jansen J., Reimer K.C., Smith J.R., Dobie R., Wilson-Kanamari J.R., Halder M., Xu Y., Kabgani N., Kaesler N., Klaus M., Gernhold L., Puelles V.G., Huber T.B., Boor P., Menzel S., Hoogenboezem R.M., Bindels E.M., Steffens J., Floege J., Schneider R.K., Saez-Rodriguez J., Henderson N.C, & Kramann R. (2020). Decoding myofibroblast origins in human kidney fibrosis. Nature, 589(7841), 281-286.
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