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3 protocols using phenylephrine p6126

1

Signaling Pathways in Cardiomyocyte Hypertrophy

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Antibodies against the following proteins were purchased from Cell Signaling Technology (Danvers, MA, USA): GCN5 (3305, 1:1000 dilution), p-TAK1 (9339, 1:1000 dilution), TAK1 (5206, 1:1000 dilution), p-ERK1/2 (4370, 1:1000 dilution), ERK1/2 (4695, 1:1000 dilution), p-JNK (4668, 1:1000 dilution), JNK (9252, 1:1000 dilution), p-p38 (4511, 1:1000 dilution), p38 (9212, 1:1000 dilution), TAB1 (3226, 1:1000 dilution), TAB2 (3,745, 1:1000 dilution), Acetylated-Lysine Antibody (9441 S, 1:1000 dilution) and GAPDH (5174, 1:1000 dilution). Antibodies against ANP (sc20158, 1:200 dilution) and β-MHC (sc53090, 1:200 dilution) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Phenylephrine (P6126) and antibody against α-actinin (A7811, 1:100 dilution) were obtained from Sigma-Aldrich (St. Louis, MO, USA). A BCA protein assay kit, obtained from Thermo Fisher (Waltham, MA, USA), was used to determine the protein concentrations. MB3 (HY-129039), NG25 (HY-15434), and 5Z-7-oxozeaenol (HY-12686) were purchased from MCE (Shanghai, China).
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2

Atenolol and Phenylephrine Assay

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Atenolol (A7655), and (‐)‐phenylephrine (P6126) were obtained from Sigma‐Aldrich (St. Louis, MO).
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3

Pharmacological Modulation of Vascular Tone

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The following reagents were purchased from Sigma-Aldrich: phenylephrine (P6126), carbachol (C4382), furegrelate (F3764), indomethacin (I7378), LNNA (N5501), and EGTA (E3889).
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