Anti pabp

Manufactured by Cell Signaling Technology

Anti-PABP is a primary antibody that recognizes the Poly(A) Binding Protein (PABP), a key regulator of mRNA translation and stability. The antibody can be used to detect and quantify PABP expression in various cell and tissue samples.

Automatically generated - may contain errors

Lab products found in correlation

3 protocols using anti pabp

Protein Detection via Western Analysis and IF

Check if the same lab product or an alternative is used in the 5 most similar protocols

The primary antibodies for the following proteins were used for Western analysis and immunofluorescence (IF): anti-Nup62 (BD Biosciences #610497, used at 1:2000 for Western), anti-Nup98 (Abcam #45584, used at 1:1000 for Western), anti-Nup153 (Abcam #96462, used at 1:1000 for Western), anti-eIF4G (BD Biosciences #610536, used at 1:1000 for Western), anti-PABP (Cell Signaling Technology #4992, used at 1:1000 for Western), anti-nucleolin (Abcam #22758, used at 1:3000 for Western), anti-hnRNP-C1/C2 (Santa Cruz #32308, used at 1:500 for IF), anti-SC35 (Sigma #4045, used at 1:500 for IF), anti-Sam68 (Santa Cruz #sc333, used at 1:500 for IF), and anti-α/β-tubulin (Cell Signaling Technology #2148, used at 1:1000 for Western). Antibodies to 3C^pro were kindly provided by S. Amineva (Madison, WI, USA; Amineva et al., 2004 (link)) and antibodies to dsRNA were kindly provided by S. Bowden (VIDRL, Melbourne, VIC, Australia).

Walker E.J., Jensen L.M., Croft S, & Ghildyal R. (2015). Variation in the nuclear effects of infection by different human rhinovirus serotypes. Frontiers in Microbiology, 6, 875.

+ Open protocol

+ Expand

Immunoblot Antibody Dilutions for Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunoblot studies were performed using the following antibodies at 1:1000 dilution: anti-DAP5 (BD Biosciences 610742); anti-eIF3d (Bethyl A301-758A); anti-eIF4A1 (Cell Signaling 2490); anti-eIF4G1 (Cell Signaling 2858); anti-rpS19 (Bethyl A304-002A); anti-eIF4E (Bethyl A301-154A); anti-HA epitope tag (Abcam 18181); anti-c-Jun (Cell Signaling 9165); anti-CDK12 (Cell Signaling 11973); anti-MMP1 (Abcam 137332); anti-GAPDH (Cell Signaling 2118S); anti-4E-BP1 (Cell Signaling 9644); anti-Lumin A (Abcam 226189); anti-AUF1 (EMD Millipore 07260); anti FXR1 (Cell Signaling 12295); anti FXR2 (Cell Signaling 7098); and anti-PABP (Cell Signaling 4992). The following antibodies were used at 1:500 dilution: anti-ETS1 (Abcam 26096); anti-OSMR (Abcam 210771); anti-EGFR (Cell Signaling 4257); anti-SERPINE2 (Abcam 75348); anti-ITGV (Abcam 124968); and anti-p53-BP1 (Abcam 21083).

de la Parra C., Ernlund A., Alard A., Ruggles K., Ueberheide B, & Schneider R.J. (2018). A widespread alternate form of cap-dependent mRNA translation initiation. Nature Communications, 9, 3068.

+ Open protocol

+ Expand

Characterization of Insulin Signaling Cascade

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following commercial antibodies were employed: mouse monoclonal antibodies anti-PTBP1, anti-V5 (Invitrogen), anti-m3G/m7G-cap (Synaptic System), anti-γ-tubulin and anti-insulin (for western blotting) (Sigma), rabbit polyclonal antibodies anti-eIF4E, anti-ph-eIF4E (Ser-209), anti-4E-BP, anti-eIF4G, anti-AKT, anti-ph-AKT (Ser-473), anti-p70S6K, anti-ph-p70S6K (Thr-389) and anti-PABP (Cell Signaling Technology), anti-CgA (Abcam), anti-PC1/3 and anti-PC2 (GeneTex), guinea pig antibody anti-insulin (for immunocytochemistry) (Abcam), goat anti-mouse, anti-rabbit and anti-guinea pig IgGs conjugated with Alexa 488 or Alexa 568 (Molecular Probes), goat anti-mouse and anti-rabbit IgGs conjugated to horseradish peroxidase (Bio-Rad). The mouse anti-ICA512 mAb has been previously described in Ref. [25] . The following reagents were from commercial sources: Rapamycin and LY294002 (Cell Signaling Technology), the eIF4E/eIF4G interaction inhibitor (Calbiochem), m⁷GpppG cap-analog (New England Biolabs), m⁷GTP-Sepharose (GE Healthcare) and Dynabeads M270 streptavidin (Invitrogen), reagents for luciferase assay (Promega), ³⁵S-methionine (Hartmann-Analytics), pro-/insulin ELISA (Mercodia) and insulin RIA (Millipore).

Knoch K.P., Nath-Sain S., Petzold A., Schneider H., Beck M., Wegbrod C., Sönmez A., Münster C., Friedrich A., Roivainen M, & Solimena M. (2014). PTBP1 is required for glucose-stimulated cap-independent translation of insulin granule proteins and Coxsackieviruses in beta cells. Molecular Metabolism, 3(5), 518-530.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!