TEM analysis was performed using a Jeol-1200 EX II-TEM microscope (Jeol, Columbia, MD, USA). Nanoparticle dispersions were 1 : 100 diluted with ultrapure Milli-Q® water, and 10 μL of each formulation was spread onto a Cu grid of 400 mesh. After incubation, the sample excess was removed with filter paper. One drop of phosphotungstic acid was added to the grid, for contrast enhancement, and incubated at 25 °C for 1 min before excess removal. Finally, the grid was dried at room temperature.
1200 ex 2 tem microscope
Manufactured by JEOL
Sourced in United States
The JEOL-1200 EX II-TEM is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of materials at the atomic scale. It is equipped with advanced optics and a high-performance electron gun, enabling the acquisition of detailed micrographs and diffraction patterns.
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9 protocols using 1200 ex 2 tem microscope
1
TEM Analysis of Nanoparticle Dispersions
2
Nanoparticle Characterization by TEM
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Transmission electron microscopy images were captured using a Jeol-1200 EX II-TEM microscope (Jeol, MA, USA). A drop (10 µL) of the nanoparticle dispersion previously diluted (1:10) with ultrapure water was spread onto a collodion-coated Cu grid (400 mesh). Excess liquid was drained with filter paper. A drop of phosphotungstic acid was added to the dispersion for contrast enhancement.
3
Physicochemical Characterization of Nanostructured Lipid Carriers
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The average hydrodynamic diameter (DH), polydispersity index (PdI), and Z-potential (ζ) were determined for NLC-Viol and NLC-Viol-Lip samples diluted 1/100 [2.0% (w/v) initial concentration and 0.02% (w/v) final concentration of MM] in ultrapure water using a Zetasizer Nano ZS series (Malvern Instruments, United Kingdom). The determinations were performed at a 633 nm (He-Ne) laser at a 173° measurement angle at 25°C by triplicate.
Transmission electron microscopy (TEM) images of the nanoparticles were acquired in a Jeol-1200 EX II-TEM microscope (Jeol, Columbia, MD, United States). NLC suspensions were diluted 1/100 [2% (w/v) and 0.02% (w/v) initial and the final concentration of MM, respectively] in ultrapure water or 10 mM phosphate buffer (pH = 7.4) followed by spreading a 10 µl sample on a 400 mesh Cu grid. The excess sample was removed with filter paper. A phosphotungstic acid drop was added to the samples for contrast enhancement.
Transmission electron microscopy (TEM) images of the nanoparticles were acquired in a Jeol-1200 EX II-TEM microscope (Jeol, Columbia, MD, United States). NLC suspensions were diluted 1/100 [2% (w/v) and 0.02% (w/v) initial and the final concentration of MM, respectively] in ultrapure water or 10 mM phosphate buffer (pH = 7.4) followed by spreading a 10 µl sample on a 400 mesh Cu grid. The excess sample was removed with filter paper. A phosphotungstic acid drop was added to the samples for contrast enhancement.
4
Transmission Electron Microscopy of Nanostructured Lipid Carriers
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Images were obtained at the transmission electron microscopy (TEM) Service of the School of Veterinary Sciences of the National University of La Plata, using a Jeol-1200 EX II-TEM microscope (Jeol, Columbia, MD, United States). The samples were diluted with ultrapure water (Milli-Q®, MA, United States). Two dilutions (1:10 and 1:500) of the optimized formulation NLC-PB and one dilution (1:10) of the non-loaded NLC (NLC-vehicle) were observed. Ten microliters of each dilution were spread onto a Cu grid of 400 mesh. After incubation, the sample excess was removed with filter paper. For contrast enhancement, one drop of phosphotungstic acid was added to the grid and incubated at 25°C for 1 min before excess removal. Finally, the grid was dried at room temperature.
5
Characterization of Nanostructured Lipid Carriers
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The average diameter and particle size distribution of NLCs were measured by dynamic light scattering (Nano ZS Zetasizer, Malvern Instruments Corp, UK) at 25 °C in polystyrene cuvettes with a thickness of 10 mm. Measurements were carried out in 10 mm path length capillary cells, using deionized water (Milli-Q®, Millipore, MA, USA). The polydispersity Index (PdI) values and the zeta potentials (ζ) were also determined. All the measurements were performed in triplicate to obtain the mean value.
TEM analysis was conducted using a Jeol-1200 EX II-TEM microscope (Jeol, MA, USA). First, the NLC formulations were diluted 10 times with ultrapure Milli-Q® water, and 10 μL of the sample was spread onto a collodion-coated Cu grid (400-mesh). Liquid excess was taken out with filter paper, and for contrast enhancement, a drop of phosphotungstic acid was added to the samples.
TEM analysis was conducted using a Jeol-1200 EX II-TEM microscope (Jeol, MA, USA). First, the NLC formulations were diluted 10 times with ultrapure Milli-Q® water, and 10 μL of the sample was spread onto a collodion-coated Cu grid (400-mesh). Liquid excess was taken out with filter paper, and for contrast enhancement, a drop of phosphotungstic acid was added to the samples.
6
TEM Imaging of Nanoparticle Dispersions
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The formulations were diluted with Milli-Q water (1:10), and a drop of the dispersion was spread onto a 400-mesh collodion-coated Cu grid (Merck KGaA, Darmstadt, Germany). Phospho-tungstic acid was added to enhance contrast. Images were observed in a Jeol-1200 EX II-TEM microscope (Jeol, MA, USA).
7
TEM Imaging of Nanoparticle Dispersions
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The nanoparticle dispersion was 10-times diluted with ultrapure water and a drop of the dispersion was spread onto a collodion-coated Cu grid (400-mesh). Liquid excess was drained with paper filter (Whatman #1) and for contrast enhancement a drop of phosphotungstic acid as added to the NLCs dispersion. Finally, TEM analysis was performed using Jeol-1200 EX II-TEM microscope (Jeol, MA, USA).
8
Nanoparticle TEM Sample Preparation
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The nanoparticle dispersion was ten times diluted ten times with ultrapure water, and a drop of the dispersion was spread onto a collodion-coated Cu grid (400 mesh). Liquid excess was drained with filter paper. One drop of phosphotungstic acid was added to the dispersion for contrast enhancement. Finally, TEM analysis was performed using a Jeol-1200 EX II-TEM microscope (Jeol, Ma, USA).
9
TEM Characterization of Nanoparticle Dispersion
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The nanoparticle dispersion was ten times diluted with ultrapure water, and a drop of the dispersion was spread onto a collodion-coated Cu grid (400-mesh). Liquid excess was drained with filter paper, and for contrast enhancement a drop of phosphotungstic acid was added to the SLN dispersion. Finally, TEM analysis was performed using Jeol-1200 EX II-TEM microscope (Jeol, MA, USA).
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