Apc cy7 conjugated anti cd45 30 f11

Manufactured by BD

APC-Cy7-conjugated anti-CD45 (30-F11) is a flow cytometry reagent. It is a monoclonal antibody that binds to the CD45 antigen and is conjugated with the APC-Cy7 fluorescent dye.

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Lab products found in correlation

Pe texas red, by Thermo Fisher Scientific (1 mentions) Lsrii flow cytometer, by Tree Star (1 mentions) Prism 6, by GraphPad (1 mentions) Facs lsr 2, by Tree Star (1 mentions) Anti f4 80, by BioLegend (1 mentions)

2 protocols using apc cy7 conjugated anti cd45 30 f11

Thymus Tissue Dissociation and Flow Cytometry

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Thymus tissue was incubated for 1 h at 37°C in collagenase digestion mixture (0.1 mg/ml collagenase type 4 in calcium-replete HBSS containing 10% FCS and 200 U/ml DNase). After homogenization and filtration, the resultant cells were stained and analyzed using an LSRII flow cytometer together with FlowJo software (Tree Star) as has been described [35 (link),36 (link)]. Biotin-conjugated anti-CD90.2 (53–2.2), eFluor605NC-conjugated anti-CD19 (1D3), APC-conjugated anti-CD4 (GK1.5), eFluor450-conjugated anti-CD8 (53–6.7), and PE-Cy7-conjugated anti-CD3 (145–2C11) were purchased from eBioscience, whereas APC-Cy7-conjugated anti-CD45 (30-F11) was obtained from BD Pharmingen. Biotin-conjugated antibodies were detected using streptavidin conjugated with PE-TexasRed (Invitrogen). Mann–Whitney t-tests (with a 95% confidence interval) were performed using Prism 6.0 (GraphPad Software, La Jolla, USA). All P-values are two-tailed (*P < 0.05, **P < 0.01, and ***P < 0.001).

Chauhan S., Diril M.K., Lee J.H., Bisteau X., Manoharan V., Adhikari D., Ratnacaram C.K., Janela B., Noffke J., Ginhoux F., Coppola V., Liu K., Tessarollo L, & Kaldis P. (2016). Cdk2 catalytic activity is essential for meiotic cell division in vivo. The Biochemical journal, 473(18), 2783-2798.

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Quantification of Pulmonary Immune Cells

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To examine the absolute number of AM and other innate immune cells, BAL cells were collected as above and perfused lungs were isolated and minced through 70 μm cell strainer using MEM. The cells were stained with FITC-conjugated anti-CD11c (HL3), PE-conjugated anti-Siglec-F (E50-2440), PerCP-conjugated anti-Ly6C (AL-21), PE-Cy7-conjugated anti-Ly6G (1A8), APC-conjugated anti-CD11b (M1-70) or APC-conjugated anti-F4/80 (BM8), and APC-Cy7-conjugated anti-CD45 (30-F11) (all from BD Biosciences except anti-F4/80 from BioLegend). The cells were acquired using FACS LSR II and flow cytometric data were analyzed by using FlowJo software (Tree Star, San Carlos, CA, United States). We defined CD45⁺Ly6C^-Ly6G^-CD11c⁺Sigleg-F⁺F4/80⁺ as AMs, CD45⁺CD11b⁺Ly6C⁺Ly6G^- for inflammatory monocytes, and CD45⁺CD11b⁺Ly6G⁺Ly6C^- for neutrophils.

Hong J.E., Kye Y.C., Park S.M., Cheon I.S., Chu H., Park B.C., Park Y.M., Chang J., Cho J.H., Song M.K., Han S.H, & Yun C.H. (2019). Alveolar Macrophages Treated With Bacillus subtilis Spore Protect Mice Infected With Respiratory Syncytial Virus A2. Frontiers in Microbiology, 10, 447.

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