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2 protocols using zinc acetate dihydrated

1

Synthesis of Self-Lighting Antiproliferative Drugs

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Diethylene glycol, zinc acetate dihydrated, and L-cysteine (nonanimal) were purchased from Sigma-Aldrich (St. Louis, MO, USA), and were used to synthesize the water-soluble zinc oxide nanoparticles. MTCP and its copper containing derivative (CuMTCP), as the optically active material, were obtained from Frontier Scientific (Logan, UT, USA). These porphyrin compounds were conjugated to cysteine-coated ZnO nanoparticles to synthesize self-lighting antiproliferative drugs. 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysulfosuccinimide (sulfo-NHS) were obtained from Sigma-Aldrich and were used for chemical conjugation of MTCP or CuMTCP to cysteine-coated ZnO nanoparticles. Roswell Park Memorial Institute Medium 1640 (RPMI) and fetal bovine serum (FBS) were purchased from Invitrogen (Carlsbad, CA, USA). The 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT), penicillin-streptomycin solution, and dimethyl sulfoxide (DMSO) were obtained from Sigma-Aldrich. Econo-Pac 10DG desalting columns were obtained from BioRad (Hercules, PA, USA).
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2

Synthesis and Characterization of Cadmium Oxide Nanocrystals

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Cadmium oxide Puratronic (CdO, 99.998%) and octadecylphosphonic acid (ODPA, 97%) were purchased from Alfa Aesar. Trioctylphosphine (TOP, 90%), Igepal CO-520 (poly(5)oxyethylene-4-nonylphenylether), thioacetamide (TA, Z99.0%), thiocarbamide (TC, Z99.0%), zinc acetate dihydrated (ZnAc, Z98.0%), octadecene (ODE, 90%) and Coumarin 6 (98%) were purchased from Sigma Aldrich. Trioctylphosphine oxide (TOPO, 98%) was purchased from Merck. Absolute ethanol (99.8%), cyclohexane (99.5%), formamide (Z99.5%), isopropanol (Z99.8%) and chloroform (99.6%) were purchased from Sharlau. ZnS, hydrated ZnSO 4 , CdSO 4 and ZnO were used as references for X-ray Absorption Near Edge Structure (XANES) spectroscopy. All chemicals were used directly without further purification.
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