Granzyme b clone ngzb

Manufactured by Thermo Fisher Scientific

Granzyme B (clone NGZB) is a lab equipment product offered by Thermo Fisher Scientific. It is a recombinant antibody that can be used to detect the presence of Granzyme B, a serine protease involved in the induction of cell apoptosis. The core function of this product is to serve as a research tool for the identification and quantification of Granzyme B in various experimental settings.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Granzyme B (43 citations) Granzyme B (NGZB, (5 citations)

3 protocols using granzyme b clone ngzb

Induction of Regulatory T Cells by CVF- and CLys-treated BMDCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

BMDCs were generated as above and incubated for 72 h with CVF or CLys. 24 h before the end of culture, cells were pulsed overnight with endotoxin‐free OVA protein (20 μg/ml). On day 0, CVF‐ and CLys‐treated BMDCs were extensively washed with PBS, and 2 × 10⁵ antigen‐pulsed cells, or PBS or naive BMDCs as control, were subcutaneously injected into separate recipient C57BL/6 mice, which had previously (24 h earlier) received intraperitoneally 1–2 × 10⁵ flow sorted naïve CD4⁺CD44^−/lo OT‐II cells. Mice were sacrificed on day 7, the inguinal lymph nodes (ILN) were removed and ILN cell suspensions, prepared as previously reported (Layland et al, 2010 (link)), were stained with CD4 (clone GK 1.5) (BioLegend), CD45.1 (clone A20), CD25 (clone PC61), FoxP3 (clone MF23) (all from BD Biosciences), and Granzyme B (clone NGZB) (eBioscience) for flow cytometry analysis of Treg induction.

Prodjinotho U.F., Gres V., Henkel F., Lacorcia M., Dandl R., Haslbeck M., Schmidt V., Winkler A.S., Sikasunge C., Jakobsson P., Henneke P., Esser‐von Bieren J, & Prazeres da Costa C. (2022). Helminthic dehydrogenase drives PGE2 and IL‐10 production in monocytes to potentiate Treg induction. EMBO Reports, 23(5), e54096.

+ Open protocol

+ Expand

Flow Cytometric Analysis of Donor Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

FACS analyses were performed as previously described^{21 (link),22 (link)}. Briefly, to analyze donor T cell or myeloid cell expansion and activation markers, splenocytes from transplanted animals were resuspended in FACS wash buffer (2% bovine serum albumin (BSA) in phosphate buffered saline (PBS)) and stained with conjugated monoclonal antibodies (moAbs). The following antibodies were used: FITC-conjugated moAbs to mouse CD45.1 (clone SF1–1.1) and CD229.1 (clone 30C7); PerCP-Cy5.5-conjugated moAbs to mouse CD4 (clone GK1.5), CD8 (clone 53–6.7) and CD11c (clone N418); PE-conjugated moAbs to mouse CD69 (clone H1.2F3), CD62L (clone MEL-14), and CD11b (clone M1/70); and APC-conjugated moAbs to mouse CD44 (clone IM7) and CD25 (clone PC61). All antibodies were purchased from Biolegend (San Diego, CA) except the FITC anti-CD229.1, which was purchased from BD bioscience (San Diego, CA). After staining, the cells were washed twice and fixed with 2% formaldehyde as described previously^{21 (link)}. Cells were analyzed using a BD Accuri C6 flow cytometer (BD Bioscience). For intracellular cytokine staining, cells were permeabilized after fixation and stained with PE-conjugated IFN-γ (clone XMG1.2, Biolenegd) and granzyme B (clone NGZB, eBioscience), APC-conjugated IL-17A (clone TC11–18H10.1, Biolegend) and perforin (clone eBioOMAK-D, eBioscience) according to manufacturer’s protocol.

Toubai T., Rossi C., Tawara I., Liu C., Zajac C., Oravecz-Wilson K., Peltier D., Sun Y., Fujiwara H., Wu S.R., Riwes M., Henig I., Kim S, & Reddy P. (2018). Murine Models of Steroid Refractory Graft-versus-Host Disease. Scientific Reports, 8, 12475.

+ Open protocol

+ Expand

Comprehensive Immunophenotyping of Influenza Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lungs were harvested at the indicated time points and RNA was extracted by TRI-Reagent (Molecular Research Center). Viral loads were determined by RT-PCR using influenza virus specific primers as previously described (30 (link)). Cells were stained as previously described (31 (link)) using monoclonal antibodies against CD69 (clone H1.2F3), Thy1.1 (clone HIS51), Thy1.2 (clone 53-2.1), CD4 (clone L3T4), CD45.1 (clone A20), CD45.2 (clone 104), CD62L (clone MEL-14), CD44 (clone 1M7), CD127 (clone A7R34), KLRG1 (clone 2F1), IFN-γ (clone XMG1.2), TNF-α (clone MP6-XT22), and Granzyme-B (clone NGZB) (all from eBioscience, San Diego, CA), CD8α (clone 53-6.7, BD Biosciences, San Jose, CA), CD25 (clone PC61, BD Biosciences), and H-2^b MHC class I tetramers loaded with immunodominant influenza virus nuclear protein (NP) epitope NP_(366-374) or OVA_(257-264) peptide. Anti-CD16/32 (Fc Block, clone 2.4G2) (BD Biosciences) was used in all stains. For intracellular cytokine and granule staining, GolgiPlug (BD Biosciences), Cytofix/Cytoperm buffer (eBioscience) and Perm/Wash buffer (eBioscience) were used according to the manufacturer’s instructions. Samples were analyzed using a FACSAria flow cytometer (BD Biosciences). All data were analyzed using FlowJo software (Treestar).

Gracias D.T., Boesteanu A.C., Fraietta J.A., Hope J.L., Carey A.J., Mueller Y.M., Kawalekar O.U., Fike A.J., June C.H, & Katsikis P.D. (2016). Phosphoinositide 3-Kinases p110δ isoform regulates CD8+ T cell responses during acute viral and intracellular bacterial infections. Journal of immunology (Baltimore, Md. : 1950), 196(3), 1186-1198.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!