Both PT and aPTT were assayed using the RecombiPlasTin (Instrumentation Laboratory, Milan, Italy) and SynthASil (Instrumentation Laboratory) on an ACL 3000 (Instrumentation Laboratory). Fibrinogen was measured using the HemosIL Fibrinogen-C XL reagent (Instrumentation Laboratory SpA). Coagulation factors were assayed using a PT-based clotting assay with the HemosIL RecombiPlasTin reagent (for FII, FV, FVII, and FX) and an aPTT-based clotting assay using the SynthASil reagent (for FVIII, FXI, FXI, and FXII). d -dimers were measured using an immunoturbidimetric method on ACL 3000. Antithrombin and protein C activity was determined using chromogenic assays (HemosIL liquid antithrombin and HemosIL protein C, respectively; Instrumentation Laboratory SpA). The tissue factor pathway inhibitor (TFPI) was measured using a TFPI Human ELISA Kit (Abcam (Cambridge, MA)) according to the manufacturer’s protocol.
Acl 3000
Manufactured by Werfen
Sourced in Italy
The ACL 3000 is a laboratory equipment designed for coagulation testing. It is used to perform coagulation assays, providing quantitative results for various coagulation parameters.
Automatically generated - may contain errors
Lab products found in correlation
Recombiplastin, by Werfen (1 mentions)
Synthasil, by Werfen (1 mentions)
Hemosil liquid antithrombin, by Werfen (1 mentions)
Hemosil protein c, by Werfen (1 mentions)
Hemosil fibrinogen c xl reagent, by Werfen (1 mentions)
Fluoroskan ascent, by Thermo Fisher Scientific (1 mentions)
Quant it picogreen dsdna reagent, by Thermo Fisher Scientific (1 mentions)
Sodium citrate tube, by BD (1 mentions)
Cell death detection elisa, by Roche (1 mentions)
Hemosil recombiplastin, by Werfen (1 mentions)
2 protocols using acl 3000
1
Coagulation Assays: A Comprehensive Approach
2
Comprehensive Coagulation and NET Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Peripheral blood was collected in sodium citrate tubes (Becton Dickinson, San Jose, CA, USA). The whole blood samples were centrifuged for 15 minutes at 1550 x g within 2 hours after sample withdrawal. The prothrombin time (PT), activated partial thromboplastin time (aPTT), and fibrinogen were assayed on an ACL 3000 automated coagulation analyzer (Instrumentation Laboratory, Milan, Italy) using HemosIL RecombiPlasTin and SynthASil reagents (Instrumentation Laboratory). Ddimer was tested by an immunoturbidimetric assay (Instrumentation Laboratory). Antithrombin and protein C activity were measured by a chromogenic assay (HemosIL Antithrombin and Protein C; Instrumentation Laboratory). The circulating levels of NET in plasma were quantified using two commercial kits; The DNA-histone complex was quantified using an ELISA kit (Cell Death Detection ELISA, Roche Diagnostics, IN, USA); the double-stranded DNA (dsDNA) was measured using Quant-iT PicoGreen dsDNA reagent (Molecular Probes, Eugene, OR, USA) and a microplate fluorometer (Fluoroskan Ascent, Thermo Fisher Scientific Inc., MA, USA) according to the manufacturer's guidelines.
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