The media ingredients including glucose, fructose, maltose, arabinose, yeast extract, nutrient agar and mineral salts [CaCl2.2H2O, CuCl, FeCl3.6H2O, FeSO4. 7H2O, AlCl3.6H2O, Na3MoO4.2H2O, Co(NO3)2.6H2O, NaCl, KH2PO4, K2HPO4, MgSO4.7H2O, ZnSO4.7H2O, MnSO4.4H2O] were obtained from Merck Chemical Co. (Darmstadt, Germany). The riboflavin standard was purchased from Sigma-Aldrich (Sigma-Aldrich Co., United States).
Riboflavin standard
Manufactured by Merck Group
Sourced in United States
The Riboflavin standard is a laboratory reference material used for the quantitative analysis of the vitamin riboflavin (also known as vitamin B2) in various sample types. It provides a stable and reliable source of the analyte to aid in the calibration and validation of analytical methods.
Automatically generated - may contain errors
Lab products found in correlation
Co no3 2 6h2o, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Kh2po4, by Merck Group (1 mentions)
K2hpo4, by Merck Group (1 mentions)
Alcl3 6h2o, by Merck Group (1 mentions)
Mgso4.7h2o, by Merck Group (1 mentions)
Znso4.7h2o, by Merck Group (1 mentions)
Acid phosphatase from potato, by Merck Group (1 mentions)
Papain from papaya latex, by Merck Group (1 mentions)
3 protocols using riboflavin standard
1
Nutrient Media Formulation for Microbial Growth
2
Riboflavin Production Quantification
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The amount of riboflavin produced was estimated after 48 h of fermentation. The fermented culture broth was centrifuged at 4800 g for 10 min. The collected supernatant was analyzed for the presence of riboflavin by using UV spectrophotometer at 444 nm. The concentration of riboflavin was calculated using extinction coefficient (1.04 × 10–2 M–1 cm–1) and the standard graph was plotted using riboflavin standard (Sigma Aldrich) (Sauer et al., 1996 (link)).
3
Determination of Riboflavin in Nutritional Formulas
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Riboflavin standard was purchased from Sigma-Aldrich (St Louis, USA, purity = 98%). Solvents were of "gradient grade for HPLC" and other chemical products were of "analytical grade" and all were purchased from Sigma-Aldrich (St Louis, USA) or VWR (Radnor, USA). For enzymatic hydrolysis, papain from Papaya latex and acid phosphatase from potato were purchased from Sigma-Aldrich. Ultrapure water from Simplicity® system (Merck Millipore, Darmstadt, Germany) was used for sample preparation and chromatographic analysis. Certified reference material (milk-based infant/adult nutritional formula powder, SRM 1849a, NIST®) was purchased from LGC Standards (Molsheim, France).
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