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Alamarblue cell viability reagent kit

Manufactured by Thermo Fisher Scientific
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AlamarBlue cell viability reagent kit is a fluorometric and colorimetric assay used to quantitatively measure cell proliferation and cytotoxicity. The reagent contains resazurin, a redox indicator that is reduced by metabolically active cells, resulting in a color change that can be measured.

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Lab products found in correlation

Nutrient broth, by BD (1 mentions) Difco, by BD (1 mentions) Nutrient agar, by BD (1 mentions)

2 protocols using alamarblue cell viability reagent kit

1

Antibacterial Evaluation of Novel Compounds

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Nutrient Agar (DIFCO, Becton Dickinson) and Nutrient Broth (DIFCO, Becton Dickinson) were used for disc diffusion as well as MIC and MBC. Muller Hinton II broth cation adjusted (Becton Dickson) and Muller Hinton Agar was used for time killing assay. AlamarBlue cell viability reagent kit (alamarBlue, Invitrogen) was used for bacteria viability assay. The 96-well microplates (NEST, Nest Biotech Co. Ltd) were used for MIC, MBC and time kill curve. SEM specimen stubs (SPI Supplies, Structure Probe, Inc., PA, USA) were used for the embedding of the bacteria sample for the SEM processing. Sodium Phosphate Monobasic (Na2HPO4, anhydrous, Fisher Scientific) and Glutaraldehyde reagent (E.M. Grade, SPI Supplies, Structure Probe, Inc., USA) were used for SEM and TEM.
Zajmi A., Mohd Hashim N., Noordin M.I., Khalifa S.A., Ramli F., Mohd Ali H, & El-Seedi H.R. (2015). Ultrastructural Study on the Antibacterial Activity of Artonin E versus Streptomycin against Staphylococcus aureus Strains. PLoS ONE, 10(6), e0128157.
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2

GLP-1 Analogues Enhance INS-1 Cell Viability

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Example 20

The cultured INS-1 cells were seeded at a density of 2×104 cells/well into 96-well plate in culture medium containing 10% fecal bovine serum. After 48 hours, the cells were further incubated for another 24 hours for serum starvation. When the cells in synchronization state, the cells cultured in medium with 30 mM glucose and treated with 100 nM GLP-1 analogues or liraglutide. The cells cultured in normal medium being used as a control.

After being incubated for 24, 48 and 72 hours, the cell viability was then determined by Alamar Blue cell viability reagent kit (Invitrogen) in accordance with the manufacturer's instruction.

The cell proliferation ratio (see FIG. 19) was a ratio of the cell viability at multiple time points compared to 0 hour. The result indicated that the cell viabilities were significantly improved by some of GLP-1 analogues, including GLP-1-Aib8-EG4-2E-2FA-C16-acid (Aib8-EG4-C16-acid), GLP-1-Aib8-EG4-2E-2FA-C16 (Aib8-EG4-C16) and GLP-1-Ala8-EG4-2E-2FA-C16 (Ala8-EG4-C16), compared with liraglutide.

US10993986B2. Pharmaceutical constructs with enhanced binding affinity with albumin (2021-05-04). Immunwork Inc. [TW]. Inventors: Tse-Wen Chang [TW], Hsing-Mao Chu [TW], Chien-Jen Lin [TW].
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