The homemade polyclonal antibody to the Vav1 DH domain (Ref. 302–5) was raised in rabbits using an MBP–Vav1 DH fusion protein purified from E. coli according to standard techniques. The antibodies to the Vav1 KR region (Ref. 97) and the tyrosine phosphorylated version of Vav1 Y174 phosphopeptide (Ref. 613) were raised in rabbits using appropriate nonphosphorylated and phosphorylated synthetic peptides, respectively [24 (link),35 (link),36 (link)]. Other antibodies used in this study include those recognizing human CD3 (UCHT1 clone, Cat. #217570; Merk–Millipore, Burlington, MA, USA), tubulin α (Cat. #CP06; Calbiochem, San Diego, CA, USA), polyhistidine residues (Cat. #H–1029; Sigma, St. Louis, MO, USA), chicken IgM (Cat. #8300–01; Southern Biotech, Birmingham, AL, USA), MBP (Cat. #M–6295; Sigma), the Myc epitope (Cat. #M5546; Sigma), and GST (Cat. #G1160; Sigma). Rhodamine-labeled phalloidin was obtained from Invitrogen (Cat. #R415; Carlsbad, CA, USA).
Chicken igm
Manufactured by Southern Biotech
Sourced in United States
Chicken IgM is a laboratory product that is used to detect and measure the presence of immunoglobulin M (IgM) in chicken samples. IgM is an antibody that is produced by the chicken's immune system in response to an initial exposure to an antigen. The Chicken IgM product can be used in various immunological assays to assess the immune status or response of chickens.
Automatically generated - may contain errors
Lab products found in correlation
Rhodamine labeled phalloidin, by Thermo Fisher Scientific (1 mentions)
Myc epitope, by Merck Group (1 mentions)
P erk, by Cell Signaling Technology (1 mentions)
α tubulin, by Santa Cruz Biotechnology (1 mentions)
Shp 1, by Cell Signaling Technology (1 mentions)
Plcγ1, by Cell Signaling Technology (1 mentions)
Slp 76, by Cell Signaling Technology (1 mentions)
Shp 2, by Abcam (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
2 protocols using chicken igm
1
Polyclonal Antibody Generation for Vav1
2
Polyclonal Antibody Production and Validation
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