Rsc 96

Chitosan powder (200-kDa molecular weight and 80% deacetylation degree) was purchased from TCI Biotechnology (Shanghai, China). GO nanosheets were obtained from Hengqiu Technology (Suzhou, China). A spontaneously immortalized Schwann cell line RSC 96 (CSTR: 19375.09.3101RATGNR6; Serial: GNR 6) was supplied by the National Collection of Authenticated Cell Cultures (Shanghai, China). Fetal bovine serum, alpha minimum essential medium (α-MEM), trypsin/EDTA solution, and penicillin-streptomycin solution were supplied by Sigma (St. Louis, MO, USA). HCl, dimethyl sulfoxide (DMSO), and NaOH were provided by Sinopharm Chemical Reagent (Beijing, China). Other chemicals were used without further purification.

The PCL (30 ​kDa) was purchased from HENGQIU (Suzhou, China). The MLT was purchased from Sigma-Aldrich (Shanghai, China), and the RGO nanoparticles were purchased from XFNANO (Nanjing, China). DCM was purchased from Aladdin (Shanghai, China), and DMF was purchased from Macklin (Shanghai, China). The RSC 96 were provided by the National Collection of Authenticated Cell Cultures (Shanghai, China). The Fetal Bovine Serum (FBS), penicillin/streptomycin solution, 0.25% Trypsin-EDTA (1x), and Dulbecco's Modified Eagle's Medium (DMEM) with 4.5 ​g/L d-Glucose were purchased from Gibco (USA). The LIVE/DEAD Cell Imaging Kit was purchased from Thermo Fisher Scientific (USA), the Annexin V-FITC Apoptosis Detection Kit was purchased from Beyotime (China), and the JC-1 Mitochondrial Membrane Potential Assay Kit was purchased from MCE (Shanghai, China). The CCK8 was purchased from Absin (Shanghai, China), and all the antibodies were purchased from Abcam (USA). The 3–0 nylon sutures, 6–0 nylon sutures, and sterile scalpel blades were purchased from Jinhuan Medical (Shanghai, China).