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Taqman universal mastermix without ung

Manufactured by Thermo Fisher Scientific
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Taqman Universal Mastermix without UNG is a pre-mixed solution containing reagents required for real-time PCR amplification. It includes a proprietary buffer system, dNTPs, and a DNA polymerase enzyme. This product does not contain uracil-N-glycosylase (UNG).

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Lab products found in correlation

Mirneasy mini kit, by Qiagen (2 mentions) Assay loading reagent, by Standard BioTools (1 mentions) Ge sample loading reagent, by Standard BioTools (1 mentions) Biomark hd system, by Standard BioTools (1 mentions) Taqman mirna reverse transcription kit, by Thermo Fisher Scientific (1 mentions) Stepone real time pcr system, by Thermo Fisher Scientific (1 mentions) Nanodrop 2000 spectrophotometer, by Thermo Fisher Scientific (1 mentions) Cfx real time pcr machine, by Bio-Rad (1 mentions) Taqman microrna reverse transcription kit, by Thermo Fisher Scientific (1 mentions) Taqman microrna assay, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Universal TaqMan MasterMix (9 citations)

3 protocols using taqman universal mastermix without ung

1

High-Throughput miRNA qPCR Profiling

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MicR qPCR was performed using a high-throughput platform BioMark™ HD System (Fluidigm Corporation) and 48.48 GE Dynamic Arrays or 96.96 GE Dynamic Arrays. A total of 5 µl of sample premix contained: 1 µl of the sample (1:20 diluted preamp cDNA), 2.5 µl of Taqman Universal Mastermix without UNG (Thermo Fisher Scientific, Inc.), 0.25 µl of 20X GE sample loading reagent (Fluidigm Corporation) and 1.25 µl of water. A total of 5 µl of assay pre-mix contained 2.5 µl of 20X Taqman miR assays and 2.5 µl of 2X assay loading reagent (Fluidigm Corporation). The thermocycling conditions were as follows: 95°C for 10 min, 40 cycles of 95°C for 15 sec and 60°C for 1 min. All data were analyzed by the 2−ΔΔCq method (25 (link)).
Sojka L., Opattova A., Bartu L., Horak J., Korenkova V., Novosadova V., Krizkova V., Bruha J., Liska V., Schneiderova M., Kubecek O., Vodickova L., Urbanova M., Simsa J., Vodicka P, & Vymetalkova V. (2022). MUC13-miRNA-4647 axis in colorectal cancer: Prospects to identifications of risk factors and clinical outcomes. Oncology Letters, 25(2), 72.
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2

Quantification of Broccoletti-miRs

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The miRNeasy® Mini Kit was used according to the manufacturer’s instructions (Qiagen, Hilden, Germany), and the RNA concentrations were measured with a NanoDrop 2000 spectrophotometer (Nano Drop Technologies, Wilmington, USA). cDNA was prepared from 10 ng of total RNA using the TaqMan® miRNA Reverse Transcription Kit (Thermo Fisher Scientific, Dreieich, Germany) according to the manufacturer’s instructions. Quantitative levels of broccoletti-miRs were measured using a relative Custom TaqMan™ assay (Assay ID: miR-D, CTXGP26; bra-miR156g-5p, CTYMJM3; Myseq-330, CT47VTH) and RNU44 as an endogenous control (Assay ID: 001094) from Life Technologies. TaqMan® Universal Master Mix (without UNG) (Thermo Fisher Scientific) and the StepOne Real-Time PCR System (Applied Biosystems, Darmstadt, Germany) were used for the PCR. Primer sequences are available upon request from Thermo Fisher Scientific.
Xiao X., Sticht C., Yin L., Liu L., Karakhanova S., Yin Y., Georgikou C., Gladkich J., Gross W., Gretz N, & Herr I. (2020). Novel plant microRNAs from broccoletti sprouts do not show cross-kingdom regulation of pancreatic cancer. Oncotarget, 11(14), 1203-1217.
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3

Quantifying miR-708-5p Expression in Cold-Exposed Adipose

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For small RNA assays, we extracted RNA from iWAT of cold-exposed mice using miRNeasy mini kit (Qiagen). Reverse transcription was conducted by using TaqMan MicroRNA reverse transcription kit (ThermoFisher) and RT primers (miR-708-5p, assay ID 002341; snoRNA202 as an internal control, assay ID 001232) included in TaqMan MicroRNA assay (ThermoFisher). Quantitative PCR reaction was set by using TaqMan Universal Master mix without UNG (ThermoFisher) and the fluorescence signals were read in a Bio-Rad CFX real-time PCR machine.
Choi K.M., Ko C.Y., An S.M., Cho S.H., Rowland D.J., Kim J.H., Fasoli A., Chaudhari A.J., Bers D.M, & Yoon J.C. (2023). Regulation of beige adipocyte thermogenesis by the cold-repressed ER protein NNAT. Molecular Metabolism, 69, 101679.
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