STV‐Na was provided by Key‐Pharma Biological Inc. LPS (Escherichia coli 0111: B4) was retrieved from Sigma Chemical Co. myeloperoxidase (MPO) antibody, F480 antibody, GAPDH antibody, NF‐κB antibody, p‐NF‐κB antibody and 4,6‐diamino‐2‐phenyl indole (DAPI) were obtained from Abcam. TLR4 antibody was obtained from Cell Signaling Technology. Inc. ROS fluorescent probes were purchased from Bestbio Biologicals Ltd. The anti‐rabbit immunoglobin G (IgG) antibody was purchased from Zhongshan Jinqiao Biological Co. ExCell Bio Co was the supplier of all enzyme‐linked immunosorbent assay (ELISA) kits. Wright–Giemsa staining solution, hematoxylin‐eosin (HE) Staining Kit, bicinchoninic acid (BCA) Protein Assay Kit, 5% bull serum albumin (BSA) Blocking Buffer, goat serum and Tris buffered saline containing 0.1%Tween 20 (TBST) were acquired from Solaibao Life Sciences Co. (Beijing, China).
Tlr4 antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The TLR4 antibody is a research-use-only laboratory tool used to detect and quantify the Toll-like receptor 4 (TLR4) protein in biological samples. TLR4 is a key component of the innate immune system, responsible for recognizing pathogen-associated molecular patterns and initiating the inflammatory response.
Automatically generated - may contain errors
Lab products found in correlation
F4 80 antibody, by Abcam (1 mentions)
Lps escherichia coli 0111 b4, by Merck Group (1 mentions)
4 6 diamino 2 phenylindole dapi, by Abcam (1 mentions)
Nf κb antibody, by Abcam (1 mentions)
Myeloperoxidase mpo antibody, by Abcam (1 mentions)
P nf κb antibody, by Abcam (1 mentions)
Gapdh antibody, by Abcam (1 mentions)
2 protocols using tlr4 antibody
1
Anti-inflammatory effects of STV-Na
2
Sepsis-associated ARDS Model in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
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A total of 36 SD rats after 2-week feeding were randomly divided into 3 groups with 12 rats in each group. The Ab (anti-TLR4 monoclonal antibody)-CLP group: TLR4 antibody (10 mg/ml; Cell Signaling Technologies, Inc., Beverly, MA, USA) was injected into the caudal vein at 1 day before model establishment, and then the model establishment of sepsis-associated ARDS was conducted. The CLP group: The same volume of normal saline was injected into the caudal vein at 1 day before model establishment, and then the model establishment of sepsis-associated ARDS was conducted. The Sham group: The same volume of normal saline was injected into the caudal vein at 1 day before model establishment, and then the abdomen of rats were opened and closed after anesthesia without CLP.
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