Immunohistochemical staining of testicular tissue samples from mouse, monkey and human was carried out as described previously (Mayer et al. 2016 (link)). For each species a minimum of four and up to 14 different sections were examined. Polyclonal rabbit anti-NLRP3 IgG (R30750, NSJ Bioreagents, San Diego, CA, USA) was used as primary antibody to stain for NLRP3. Negative controls consisted of omission of the primary antibody, of incubation with rabbit IgG or non-immune serum instead of the primary antiserum. Sections were counterstained with hematoxylin and visualized using a Leica DM2500 microscope equipped with a DMC2900 CMOS camera or an Zeiss Axiovert microscope with an Insight Camera (18.2 Color Mosaik) and Spot advanced software 4.6 (SPOT Imaging Solutions, Sterling Heights, MI, USA).
Dm2500 microscope
Manufactured by Zeiss
Sourced in United States
The Zeiss DM2500 microscope is a high-performance optical instrument designed for a variety of laboratory applications. It features advanced optics, precise controls, and reliable performance to support various scientific and research needs.
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Lab products found in correlation
2 protocols using dm2500 microscope
1
Immunohistochemical Analysis of NLRP3 in Testis
2
Quantitative Confocal Imaging of Retinal Cell Types
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Images were taken on a Leica DM2500 microscope, a Zeiss LSM 800 confocal microscope, or a Zeiss LSM 880 confocal microscope at Northeastern University Institute for Chemical Imaging of Living Systems (CILS). Confocal images were z-stacks and imaging parameters were the same for all the images within an experiment.
Cell types were quantified in FIJI, using the cell counter plugin. In the 377 dpi experiment, cells of each type were counted within their respective nuclear layer (INL for Lhx, Pkcα, and Glyt1; ONL for Rhodopsin; GCL, INL, and ONL for Glast; RPE for Rpe65) and normalized to the area of DAPI in those layers. Retinal thickness was measured using the "length" tool, with two measurements per each retinal section.
The data were analyzed with either Student's two-tailed t-test in Excel or with ANOVA and post-hoc Tukey's test in JMP 21 statistical software. We used p=0.05 as the significance threshold. Graphs were created using JMP 21. Images were processed in FIJI to improve brightness and contrast, and figures were assembled in Adobe Illustrator 2021.
Cell types were quantified in FIJI, using the cell counter plugin. In the 377 dpi experiment, cells of each type were counted within their respective nuclear layer (INL for Lhx, Pkcα, and Glyt1; ONL for Rhodopsin; GCL, INL, and ONL for Glast; RPE for Rpe65) and normalized to the area of DAPI in those layers. Retinal thickness was measured using the "length" tool, with two measurements per each retinal section.
The data were analyzed with either Student's two-tailed t-test in Excel or with ANOVA and post-hoc Tukey's test in JMP 21 statistical software. We used p=0.05 as the significance threshold. Graphs were created using JMP 21. Images were processed in FIJI to improve brightness and contrast, and figures were assembled in Adobe Illustrator 2021.
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