Eiger r 4 m

All room temperature X-ray crystallographic data was collected with a Rigaku HighFlux HomeLab instrument equipped with a MicroMax-007 HF X-ray generator, Osmic VariMax optics, and a DECTRIS Eiger R 4 M hybrid photon counting detector. X-ray diffraction data were integrated using the CrysAlis Pro software suite (Rigaku Inc., The Woodlands, TX) then reduced and scaled using Aimless^{65 (link)} from the CCP4 suite^{66 (link)}. Structures were solved by molecular replacement using Phaser^{67 (link)} from CCP4 using PDB code 7N8C^{34 (link)}. Each model was iteratively refined with Phenix.refine from the PHENIX suite^{68 (link),69 (link)} and COOT^{70 (link),71 (link)}. Geometry validation was aided by Molprobity^{72 (link)}. All ligand restraints were generated with eLBOW^{73 (link)} using geometry optimized by quantum mechanical calculations in Gaussian 16 at B3LYP/6-31 g(d,p) level of theory⁷⁴ . Final data collection and refinement statistics can be found in Supplementary Table 2.

All room temperature X-ray crystallographic data was collected with a Rigaku HighFlux HomeLab instrument equipped with a MicroMax-007 HF X-ray generator, Osmic VariMax optics, and a DECTRIS Eiger R 4M hybrid photon counting detector. X-ray diffraction data were integrated using the CrysAlis Pro software suite (Rigaku Inc., The Woodlands, TX) then reduced and scaled using Aimless (Evans & Murshudov, 2013 (link)) from the CCP4 suite (Winn et al., 2011 (link)). Structures were solved by molecular replacement using Phaser (McCoy et al., 2007 (link)) from CCP4 using PDB code 7N8C (Kneller et al., 2021b (link)). Each model was iteratively refined with Phenix.refine from the PHENIX suite (Adams et al., 2010 (link); Liebschner et al., 2019 (link)) and COOT (Emsley et al., 2010 (link); Casañal et al., 2020 (link)). Geometry validation was aided by Molprobity (Chen et al., 2010 (link)). All ligand restraints were generated with eLBOW (Moriarty et al., 2009 (link)) using geometry optimized by quantum mechanical calculations in Gaussian 16 at B3LYP/6–31g(d,p) level of theory (Frisch et al., 2016 ). Final data collection and refinement statistics can be found in Table S2.