Trichloroacetic acid tca

Bovine serum albumin, quercetin, aluminium chloride, 5:5-dithiobis-2-nitrobenzoate, acetylcholine, and epinephrine bitartrate were purchased from Sigma. Trichloroacetic acid (TCA) and glycine were purchased from Vetec. The compounds n-butanol and Na₂HPO₄ were purchased from Synth.
Furthermore, the present work employed the reagents NaH₂PO₄H₂O (Cromoline), hydrogen peroxide (CRQ), thiobarbituric acid (TBA) (TediaBrasil), and coomassie brilliant blue (Amresco). All electrolyte salts, solvents, and reagents were of analytical grade. Electrolyte solutions were prepared with double distilled Milli-Q water (conductivity ≤ 0.1 μS cm⁻¹) (Millipore S. A., Molsheim, France).

Bovine serum albumin, gallic acid, rutin and quercetin, aluminium chloride, acetylcholine, 5,5′-dithiobis(2-nitrobenzoic acid) (DNTB), 1,5-bis(4-allyldimethylammoniumphenyl)pentan-3-one dibromide (BW284c51), tetraisopropyl pyrophosphoramide (iso-OMPA), acetylthiocholine iodide, S-butyrylthiocholine iodide, epinephrine bitartrate, and eosin were purchased from Sigma.
This work also made use of hydrogen peroxide (CRQ), trichloroacetic acid (TCA) (Vetec), thiobarbituric acid (TBA) (TediaBrasil), n-butanol (Synth), glycine (Vetec), NaH₂PO₄H₂O (Cromoline), Na₂HPO₄ (Synth), coomassie brilliant blue (Amresco), ethyl alcohol (Synth), xylene (Vetec), Paraplast (Merck), and hematoxylin (Merck).
All electrolyte salts, solvents and reagents were of analytical grade. Electrolyte solutions were prepared with double distilled Milli-Q water (conductivity ≤ 0.1 μS·cm⁻¹) (Millipore S. A., Molsheim, France).