For anti-double stranded (ds)DNA and anti-Histone ELISA, Immulon II plates (Dynatech) precoated with BSA were coated individually with 50 μg/ml calf thymus DNA (Sigma-Aldrich) or 50ug/ml calf thymus histone (Sigma-Aldrich) respectively. Serum was diluted and assayed for autoantigen reactivity against the plates described above by incubation overnight at 4°C. Bound IgG was detected with a goat polyclonal HRP–anti-mouse IgG detection antibody (SouthernBiotech) and visualized at 450nm using a tetramethylbenzidine (TMB) substrate (Sigma-Aldrich).
Hrp anti mouse igg detection antibody
Manufactured by Southern Biotech
The HRP–anti-mouse IgG detection antibody is a purified antibody that is conjugated with horseradish peroxidase (HRP). It is designed to detect and bind to mouse immunoglobulin G (IgG) in various immunoassay applications.
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2 protocols using hrp anti mouse igg detection antibody
1
Autoantigen Reactivity ELISA Protocol
2
Autoantigen Reactivity ELISA Protocol
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For anti-double stranded (ds)DNA and anti-Histone ELISA, Immulon II plates (Dynatech) precoated with BSA were coated individually with 50 μg/ml calf thymus DNA (Sigma-Aldrich) or 50ug/ml calf thymus histone (Sigma-Aldrich) respectively. Serum was diluted and assayed for autoantigen reactivity against the plates described above by incubation overnight at 4°C. Bound IgG was detected with a goat polyclonal HRP–anti-mouse IgG detection antibody (SouthernBiotech) and visualized at 450nm using a tetramethylbenzidine (TMB) substrate (Sigma-Aldrich).
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