Donor chondrocytes were isolated as previously described. Chondrocytes (5 × 105/well) were at passage 2 and grown on ProNectin F-coated Bioflex six-well culture plates (Flexcell International) to 80% confluence. CTS experiments were performed using an FX-5000 Flexercell system (Flexcell International). Our previous study selected the pre-optimized magnitudes of CTS, so the chondrocytes were enforced at 10% elongation (0.5 Hz) for the indicated times.34 (link), 35 (link)
Pronectin f coated bioflex six well culture plates
Manufactured by Flexcell
The ProNectin F-coated Bioflex six-well culture plates are designed for in vitro cell culture applications. The plates are coated with ProNectin F, a synthetic peptide that promotes cell attachment and growth. The six-well format provides a consistent and controlled environment for culturing cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pronectin f coated bioflex six well culture plates
1
Cyclic Tensile Stimulation of Chondrocytes
2
Chondrocyte Isolation and Interleukin-1β Treatment
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Donor chondrocytes were isolated as previously described (Oseni et al. 2013 (link)). Chondrocytes (5 × 105/well) were obtained at passage 2 and cultured in ProNectin F-coated Bioflex six-well culture plates (Flexcell International) to 80% confluence (Liu et al. 2017 (link)). The chondrocytes were treated with 10 ng/ml of interleukin (IL)-1β in the culture medium for 12 h as per a previous study (Wu et al. 2020 (link)). Untreated chondrocytes were used as controls.
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