Complete mtesr medium

Manufactured by STEMCELL

Sourced in Canada

Complete mTeSR medium is a serum-free, animal component-free culture medium for the maintenance of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) in an undifferentiated state.

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Lab products found in correlation

Matrigel, by Corning (4 mentions) Relesr, by STEMCELL (2 mentions) Cryostor 10 freezing media, by STEMCELL (2 mentions) Dulbecco phosphate buffered saline (dpbs), by STEMCELL (1 mentions) Rho associated kinase rock inhibitor y 27632, by STEMCELL (1 mentions) Accutase, by STEMCELL (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dispase, by Corning (1 mentions) Dulbecco s modified eagle medium with glutamax, by Thermo Fisher Scientific (1 mentions) Gentamycin amphotericin, by Thermo Fisher Scientific (1 mentions) Gentamycin amphotericin, by STEMCELL (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by STEMCELL (1 mentions)

Close spelling variants of this product

MTeSR™1 Complete Medium Complete mTeSR™ Plus medium MTeSR Complete MTeSR medium

5 protocols using complete mtesr medium

Culturing Pluripotent Stem Cells and Fibroblasts

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Human embryonic stem cell lines H9 (Wicell, Madison, WI, USA), H1 (Wicell, WA01) and induced pluripotent human stem cells BJ-EOS clone 4YA (provided by the Ellis lab) [64 (link)] were cultured in mTeSR complete medium (Stem Cell Technologies, Vancouver, Canada) supplemented with 50 units/mL penicillin and 50 mg/mL streptomycin. These cells were cultured on Matrigel (Corning, New York, NY, USA) treated 60 mm plates as per the manufacturers specifications [64 (link)].
Cells were passaged by washing the cultures with Dulbecco’s phosphate-buffered saline without calcium and magnesium (DPBS, Stem Cell Technologies) twice followed by accutase (Stem Cell Technologies) treatment. Once the cells were in a single cell suspension fresh medium was used to inhibit accutase activity. Cells were then counted and aliquoted for subsequent experiments. Cell were maintained in medium containing 10 μM Rho-associated kinase (ROCK) inhibitor (Y-27632; Stem Cell Technologies) for 24 h after passaging and then returned to their regular medium.
Human foreskin fibroblast cells (HFF) [64 (link)] were cultured on gelatin coated plates in high glucose (25 mM) DMEM medium supplemented with; 15% (v/v) embryonic stem cell FBS, 1.0 mM sodium pyruvate, 0.1 mM non-essential amino acid and 0.1 mM 2-mercaptoethanol.

Bourhill T., Rohani L., Kumar M., Bose P., Rancourt D, & Johnston R.N. (2023). Modulation of Reoviral Cytolysis (II): Cellular Stemness. Viruses, 15(7), 1473.

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Establishing Primary Cell Lines from HPWS

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Primary cell lines were derived from 3 fresh HPWS tissue samples from the original cohort of 15 patients. Samples were cut into small pieces and incubated between layers of Matrigel (cat#354234, Corning, Tewksbury, MA) in a 24-well plate with a media containing Dulbecco's Modified Eagle Medium with Glutamax (cat#10569010, Gibco, Rockford, IL), and supplemented with 2% penicillin-streptomycin (cat#15140122, Gibco) and 0.2% gentamycin-amphotericin (cat#R01510, Gibco). Once sufficient cell growth was achieved to support transfer to a monolayer culture, cells were extracted by dissolving the Matrigel with Dispase (cat#354235, Corning) and transferred to an adherent culture flask with media containing Dulbecco's Modified Eagle Medium with Glutamax supplemented with 10% fetal bovine serum (cat#10091148, Gibco), 5% mTeSR Complete Medium (cat#85850, STEMCELL Technologies, Vancouver, BC, Canada), 1% penicillinstreptomycin, and 0.2% gentamycin-amphotericin in a humidified incubator at 37°C and 5% CO 2 . Cells were expanded in culture and harvested at passages 5 or 6.

Williams J.K., Brasch H.D., Bockett N., Patel J., Paterson E., Davis P.F., & Tan S.T. (2021). Embryonic Stem Cell-like Population in Hypertrophic Port-wine Stain. Journal of Vascular Anomalies, 2(1), e006-e006.

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mRNA Transfection of iPSCs

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Example 7

mRNA Transfection

Induced pluripotent stem cells (iPSCs) were generated from reprogrammed donor-derived fibroblasts using non-integrating oriP/EBNA1{Yu, 2009, Human induced pluripotent stem cells free of vector and transgene sequences} plasmids. Cells were maintained on Corning® Growth Factor Reduced (GFR) Basement Membrane Matrix (#354230) and maintained in Complete mTeSR Medium (Basal medium with supplements) (StemCell Technologies, #05850). Three iPSC clones, from three individual donors, were treated with 250 ng/mL GFP mRNA (CleanCap® EGFP mRNA CleanCap® Enhanced Green Fluorescent Protein mRNA (Trilink Biotechnologies, San Diego, Calif.)) lipid particles and 100 ng/mL ApoE for a period of 48 hours. The 996 bp mRNA is represented as Seq Id No. 2. The total number of cells were determined using a DAPI nuclear label, and the percentages of GFP-expressing cells relative to the total number of cells per well were then calculated. As shown in FIG. 7, the lipid particles made from Lipid Mix L showed the highest ratio of GFP-expressing iPSCs as compared to the untreated and Lipid Mix A groups (40.4% vs 8.36%). The graph represents the mean percentage GFP-positive cells per treatment group. This experiment illustrates that mRNA as well as plasmid transfection is favorably accomplished by the lipid mixes of the invention, and that iPSC can be successfully transfected.

US11679159B2. Compositions for transfecting resistant cell types (2023-06-20). Precision Nanosystems ULC [CA]. Inventors: Anitha Thomas [CA], Andrew William Brown [CA], Rebecca Anne Grace De Souza [CA], Tara L. Fernandez [CA].

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Induced Pluripotent Stem Cell Culture

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iPSCs were cultured on Matrigel (Corning) coated dishes and maintained in complete mTeSR medium (Stemcell technologies). iPSCs were passaged every 6 days using ReLeSR (Stemcell technologies) following manufacturer’s protocol. For immunofluorescence characterization, iPSCs were passaged onto Matrigel coated coverslips and fixed after 24 hours. For cryostorage of iPSCs, cells were treated with ReLeSR, spun down and resuspended in cryoSTOR-10 freezing media (Stemcell technologies) and samples were stored in liquid nitrogen.

Raj N., McEachin Z.T., Harousseau W., Zhou Y., Zhang F., Merritt-Garza M.E., Taliaferro J.M., Kalinowska M., Marro S.G., Hales C.M., Berry-Kravis E., Wolf-Ochoa M.W., Martinez-Cerdeño V., Wernig M., Chen L., Klann E., Warren S.T., Jin P., Wen Z, & Bassell G.J. (2021). Cell-type-specific profiling of human cellular models of fragile X syndrome reveal PI3K-dependent defects in translation and neurogenesis. Cell reports, 35(2), 108991.

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Induced Pluripotent Stem Cell Culture

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