Anti dnam 1

Manufactured by BioLegend

Anti-DNAM-1 is a lab reagent used to detect and study the DNAM-1 protein, also known as CD226, which is expressed on the surface of various immune cells. This reagent can be used in flow cytometry, immunohistochemistry, and other applications to identify and analyze DNAM-1-expressing cells.

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DNAM-1 (8 citations)

2 protocols using anti dnam 1

Comprehensive NK Cell Phenotyping

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The following fluorescently conjugated antibodies were used for phenotypic analysis of NK cells: anti-LFA-1 (363404); anti-CXCR3 (353720); anti-PD-1 (329906); anti-CD107a (328612); anti-NKP44 (325116); anti-CD158e1 (312706); anti-CD158d (347006); anti-CD27 (356412); anti-CCR4 (359412); anti-DNAM-1 (338316); anti-CD16 (302040); anti-Granzyme B (515406); anti-CD62L (304806); anti-CD69 (310910); anti-NKP80 (346706); anti-NKP30 (325210); anti-CD158f (341304); anti-CD158b (312612); anti-Tim-3 (345012); anti-CD94 (305504); anti-TIGIT (372706); anti-TRAIL (308206); anti-CD57 (322306); anti-CX3CR1 (341610); anti-NKG2D (320808); anti-Perforin (353310); anti-Ki67 (350504); anti-IFN-γ (506518); anti-CD94 (305506); anti-NKp46 (331916); anti-CTLA4 (369614); anti-CD96 (338416); anti-41BB (309818); anti-CD25 (356108) were purchased from Biolegend. anti-CD159a/NKG2A (FAB1059P) and anti-NKG2C (FAB138G) were purchased from R&D.
The following fluorescently conjugated antibodies were used to identify immune cell types in eNK or PBMC: anti-human Lineage Cocktail (348803); anti-CD56 (362550); anti-CD3 (300430); anti-CD33 (366620); anti-HLA-DR (307606); anti-CD14 (301836); anti-CD19 (302242); anti-CD11b (301322); anti-CD25 (356108); and anti-FOXP3 (320108) were purchased from Biolegend, San Diego, CA, USA.

Chen M., Li Y., Wu Y., Xie S., Ma J., Yue J., Lv R., Tian Z., Fang F, & Xiao W. (2021). Anti-Tumor Activity of Expanded PBMC-Derived NK Cells by Feeder-Free Protocol in Ovarian Cancer. Cancers, 13(22), 5866.

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Cytotoxicity Assay of NK Cells

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cNK and ML NK cells were incubated with tumor targets at 5:1 ET ratio as previously described.^{13 (link),14 (link)} When indicated, cNK and ML NK cells were pre-incubated with anti-NKG2D, anti-NKp46, anti-NKp30, and anti-DNAM1 (5 µg/mL; all Biolegend) or a combination of anti-NKG2D, anti-NKp46 and anti-DNAM for 30 minutes before co-incubation with tumor targets. Cells incubated with Isotype control were used as control. At the end, the cells were stained with anti-CD56 (clone N901), anti-CD3 (clone UCHT1), anti-NKG2A (clone Z199), and anti-CD45 (clone J33) followed by intracellular staining with anti-IFN-γ (clone B27). Data was acquired in a Gallios flow cytometer (Beckman Coulter) and analyzed using FlowJo software (Tree Star v10.8).

Marin N.D., Becker-Hapak M., Song W.M., Alayo Q.A., Marsala L., Sonnek N., Berrien-Elliott M.M., Foster M., Foltz J.A., Tran J., Wong P., Cubitt C.C., Pence P., Hwang K., Zhou A.Y., Jacobs M.T., Schappe T., Russler-Germain D.A., Fields R.C., Ciorba M.A, & Fehniger T.A. (2024). Memory-like differentiation enhances NK cell responses against colorectal cancer. Oncoimmunology, 13(1), 2348254.

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